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Growth Characteristics of Chlorella sorokiniana in a Photobioreactor during the Utilization of Different Forms of Nitrogen at Various Temperatures

The cultivation of microalgae requires the selection of optimal parameters. In this work, the effect of various forms of nitrogen on the growth and productivity of Chlorella sorokiniana AM-02 when cultivated at different temperatures was evaluated. Regardless of the temperature conditions, the highe...

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Autores principales: Ziganshina, Elvira E., Bulynina, Svetlana S., Ziganshin, Ayrat M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031775/
https://www.ncbi.nlm.nih.gov/pubmed/35448814
http://dx.doi.org/10.3390/plants11081086
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author Ziganshina, Elvira E.
Bulynina, Svetlana S.
Ziganshin, Ayrat M.
author_facet Ziganshina, Elvira E.
Bulynina, Svetlana S.
Ziganshin, Ayrat M.
author_sort Ziganshina, Elvira E.
collection PubMed
description The cultivation of microalgae requires the selection of optimal parameters. In this work, the effect of various forms of nitrogen on the growth and productivity of Chlorella sorokiniana AM-02 when cultivated at different temperatures was evaluated. Regardless of the temperature conditions, the highest specific growth rate of 1.26 day(−1) was observed in modified Bold’s basal medium (BBM) with NH(4)(+) as a nitrogen source, while the highest specific growth rate in BBM with NO(3)(−) as a nitrogen source achieved only 1.07 day(−1). Moreover, C. sorokiniana grew well in medium based on anaerobic digester effluent (ADE; after anaerobic digestion of chicken/cow manure) with the highest growth rate being 0.92 day(−1). The accumulation of proteins in algal cells was comparable in all experiments and reached a maximum of 42% of dry weight. The biomass productivity reached 0.41–0.50 g L(−1) day(−1) when cultivated in BBM, whereas biomass productivity of 0.32–0.35 g L(−1) day(−1) was obtained in ADE-based medium. The results, based on a bacterial 16S rRNA gene sequencing approach, revealed the growth of various bacterial species in ADE-based medium in the presence of algal cells (their abundance varied depending on the temperature regimen). The results indicate that biomass from C. sorokiniana AM-02 may be sustainable for animal feed production considering the high protein yields.
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spelling pubmed-90317752022-04-23 Growth Characteristics of Chlorella sorokiniana in a Photobioreactor during the Utilization of Different Forms of Nitrogen at Various Temperatures Ziganshina, Elvira E. Bulynina, Svetlana S. Ziganshin, Ayrat M. Plants (Basel) Article The cultivation of microalgae requires the selection of optimal parameters. In this work, the effect of various forms of nitrogen on the growth and productivity of Chlorella sorokiniana AM-02 when cultivated at different temperatures was evaluated. Regardless of the temperature conditions, the highest specific growth rate of 1.26 day(−1) was observed in modified Bold’s basal medium (BBM) with NH(4)(+) as a nitrogen source, while the highest specific growth rate in BBM with NO(3)(−) as a nitrogen source achieved only 1.07 day(−1). Moreover, C. sorokiniana grew well in medium based on anaerobic digester effluent (ADE; after anaerobic digestion of chicken/cow manure) with the highest growth rate being 0.92 day(−1). The accumulation of proteins in algal cells was comparable in all experiments and reached a maximum of 42% of dry weight. The biomass productivity reached 0.41–0.50 g L(−1) day(−1) when cultivated in BBM, whereas biomass productivity of 0.32–0.35 g L(−1) day(−1) was obtained in ADE-based medium. The results, based on a bacterial 16S rRNA gene sequencing approach, revealed the growth of various bacterial species in ADE-based medium in the presence of algal cells (their abundance varied depending on the temperature regimen). The results indicate that biomass from C. sorokiniana AM-02 may be sustainable for animal feed production considering the high protein yields. MDPI 2022-04-16 /pmc/articles/PMC9031775/ /pubmed/35448814 http://dx.doi.org/10.3390/plants11081086 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ziganshina, Elvira E.
Bulynina, Svetlana S.
Ziganshin, Ayrat M.
Growth Characteristics of Chlorella sorokiniana in a Photobioreactor during the Utilization of Different Forms of Nitrogen at Various Temperatures
title Growth Characteristics of Chlorella sorokiniana in a Photobioreactor during the Utilization of Different Forms of Nitrogen at Various Temperatures
title_full Growth Characteristics of Chlorella sorokiniana in a Photobioreactor during the Utilization of Different Forms of Nitrogen at Various Temperatures
title_fullStr Growth Characteristics of Chlorella sorokiniana in a Photobioreactor during the Utilization of Different Forms of Nitrogen at Various Temperatures
title_full_unstemmed Growth Characteristics of Chlorella sorokiniana in a Photobioreactor during the Utilization of Different Forms of Nitrogen at Various Temperatures
title_short Growth Characteristics of Chlorella sorokiniana in a Photobioreactor during the Utilization of Different Forms of Nitrogen at Various Temperatures
title_sort growth characteristics of chlorella sorokiniana in a photobioreactor during the utilization of different forms of nitrogen at various temperatures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031775/
https://www.ncbi.nlm.nih.gov/pubmed/35448814
http://dx.doi.org/10.3390/plants11081086
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