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Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy

Allergy to Polistes dominula (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnos...

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Autores principales: Grosch, Johannes, Lesur, Antoine, Kler, Stéphanie, Bernardin, François, Dittmar, Gunnar, Francescato, Elisabetta, Hewings, Simon J., Jakwerth, Constanze A., Zissler, Ulrich M., Heath, Matthew D., Ollert, Markus, Kramer, Matthias F., Hilger, Christiane, Bilò, Maria Beatrice, Schmidt-Weber, Carsten B., Blank, Simon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031911/
https://www.ncbi.nlm.nih.gov/pubmed/35448893
http://dx.doi.org/10.3390/toxins14040284
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author Grosch, Johannes
Lesur, Antoine
Kler, Stéphanie
Bernardin, François
Dittmar, Gunnar
Francescato, Elisabetta
Hewings, Simon J.
Jakwerth, Constanze A.
Zissler, Ulrich M.
Heath, Matthew D.
Ollert, Markus
Kramer, Matthias F.
Hilger, Christiane
Bilò, Maria Beatrice
Schmidt-Weber, Carsten B.
Blank, Simon
author_facet Grosch, Johannes
Lesur, Antoine
Kler, Stéphanie
Bernardin, François
Dittmar, Gunnar
Francescato, Elisabetta
Hewings, Simon J.
Jakwerth, Constanze A.
Zissler, Ulrich M.
Heath, Matthew D.
Ollert, Markus
Kramer, Matthias F.
Hilger, Christiane
Bilò, Maria Beatrice
Schmidt-Weber, Carsten B.
Blank, Simon
author_sort Grosch, Johannes
collection PubMed
description Allergy to Polistes dominula (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnostic and therapeutic approaches, the aim was to compare five therapeutic preparations for VIT of P. dominula venom allergy available in Spain. Products from five different suppliers were analyzed by SDS-PAGE and LC-MS/MS and compared with a reference venom sample. Three products with P. dominula venom and one product with a venom mixture of American Polistes species showed a comparable band pattern in SDS-PAGE as the reference sample and the bands of the major allergens phospholipase A1 and antigen 5 were assignable. The other product, which consists of a mixture of American Polistes species, exhibited the typical band pattern in one, but not in another sample from a second batch. All annotated P. dominula allergens were detected at comparable levels in LC-MS/MS analysis of products containing P. dominula venom. Due to a lack of genomic information on the American Polistes species, the remaining products were not analyzed by this method. The major Polistes allergens were present in comparable amounts in the majority, but not in all investigated samples of venom preparations for VIT of P. dominula venom allergy.
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spelling pubmed-90319112022-04-23 Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy Grosch, Johannes Lesur, Antoine Kler, Stéphanie Bernardin, François Dittmar, Gunnar Francescato, Elisabetta Hewings, Simon J. Jakwerth, Constanze A. Zissler, Ulrich M. Heath, Matthew D. Ollert, Markus Kramer, Matthias F. Hilger, Christiane Bilò, Maria Beatrice Schmidt-Weber, Carsten B. Blank, Simon Toxins (Basel) Article Allergy to Polistes dominula (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnostic and therapeutic approaches, the aim was to compare five therapeutic preparations for VIT of P. dominula venom allergy available in Spain. Products from five different suppliers were analyzed by SDS-PAGE and LC-MS/MS and compared with a reference venom sample. Three products with P. dominula venom and one product with a venom mixture of American Polistes species showed a comparable band pattern in SDS-PAGE as the reference sample and the bands of the major allergens phospholipase A1 and antigen 5 were assignable. The other product, which consists of a mixture of American Polistes species, exhibited the typical band pattern in one, but not in another sample from a second batch. All annotated P. dominula allergens were detected at comparable levels in LC-MS/MS analysis of products containing P. dominula venom. Due to a lack of genomic information on the American Polistes species, the remaining products were not analyzed by this method. The major Polistes allergens were present in comparable amounts in the majority, but not in all investigated samples of venom preparations for VIT of P. dominula venom allergy. MDPI 2022-04-15 /pmc/articles/PMC9031911/ /pubmed/35448893 http://dx.doi.org/10.3390/toxins14040284 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grosch, Johannes
Lesur, Antoine
Kler, Stéphanie
Bernardin, François
Dittmar, Gunnar
Francescato, Elisabetta
Hewings, Simon J.
Jakwerth, Constanze A.
Zissler, Ulrich M.
Heath, Matthew D.
Ollert, Markus
Kramer, Matthias F.
Hilger, Christiane
Bilò, Maria Beatrice
Schmidt-Weber, Carsten B.
Blank, Simon
Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy
title Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy
title_full Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy
title_fullStr Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy
title_full_unstemmed Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy
title_short Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy
title_sort allergen content of therapeutic preparations for allergen-specific immunotherapy of european paper wasp venom allergy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031911/
https://www.ncbi.nlm.nih.gov/pubmed/35448893
http://dx.doi.org/10.3390/toxins14040284
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