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Construction of Multiple Logic Circuits Based on Allosteric DNAzymes

In DNA computing, the implementation of complex and stable logic operations in a universal system is a critical challenge. It is necessary to develop a system with complex logic functions based on a simple mechanism. Here, the strategy to control the secondary structure of assembled DNAzymes’ conser...

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Autores principales: Liu, Xin, Zhang, Qiang, Zhang, Xun, Liu, Yuan, Yao, Yao, Kasabov, Nikola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032175/
https://www.ncbi.nlm.nih.gov/pubmed/35454084
http://dx.doi.org/10.3390/biom12040495
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author Liu, Xin
Zhang, Qiang
Zhang, Xun
Liu, Yuan
Yao, Yao
Kasabov, Nikola
author_facet Liu, Xin
Zhang, Qiang
Zhang, Xun
Liu, Yuan
Yao, Yao
Kasabov, Nikola
author_sort Liu, Xin
collection PubMed
description In DNA computing, the implementation of complex and stable logic operations in a universal system is a critical challenge. It is necessary to develop a system with complex logic functions based on a simple mechanism. Here, the strategy to control the secondary structure of assembled DNAzymes’ conserved domain is adopted to regulate the activity of DNAzymes and avoid the generation of four-way junctions, and makes it possible to implement basic logic gates and their cascade circuits in the same system. In addition, the purpose of threshold control achieved by the allosteric secondary structure implements a three-input DNA voter with one-vote veto function. The scalability of the system can be remarkably improved by adjusting the threshold to implement a DNA voter with 2n + 1 inputs. The proposed strategy provides a feasible idea for constructing more complex DNA circuits and a highly integrated computing system.
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spelling pubmed-90321752022-04-23 Construction of Multiple Logic Circuits Based on Allosteric DNAzymes Liu, Xin Zhang, Qiang Zhang, Xun Liu, Yuan Yao, Yao Kasabov, Nikola Biomolecules Article In DNA computing, the implementation of complex and stable logic operations in a universal system is a critical challenge. It is necessary to develop a system with complex logic functions based on a simple mechanism. Here, the strategy to control the secondary structure of assembled DNAzymes’ conserved domain is adopted to regulate the activity of DNAzymes and avoid the generation of four-way junctions, and makes it possible to implement basic logic gates and their cascade circuits in the same system. In addition, the purpose of threshold control achieved by the allosteric secondary structure implements a three-input DNA voter with one-vote veto function. The scalability of the system can be remarkably improved by adjusting the threshold to implement a DNA voter with 2n + 1 inputs. The proposed strategy provides a feasible idea for constructing more complex DNA circuits and a highly integrated computing system. MDPI 2022-03-24 /pmc/articles/PMC9032175/ /pubmed/35454084 http://dx.doi.org/10.3390/biom12040495 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xin
Zhang, Qiang
Zhang, Xun
Liu, Yuan
Yao, Yao
Kasabov, Nikola
Construction of Multiple Logic Circuits Based on Allosteric DNAzymes
title Construction of Multiple Logic Circuits Based on Allosteric DNAzymes
title_full Construction of Multiple Logic Circuits Based on Allosteric DNAzymes
title_fullStr Construction of Multiple Logic Circuits Based on Allosteric DNAzymes
title_full_unstemmed Construction of Multiple Logic Circuits Based on Allosteric DNAzymes
title_short Construction of Multiple Logic Circuits Based on Allosteric DNAzymes
title_sort construction of multiple logic circuits based on allosteric dnazymes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032175/
https://www.ncbi.nlm.nih.gov/pubmed/35454084
http://dx.doi.org/10.3390/biom12040495
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