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Thiolated Chitosan Conjugated Liposomes for Oral Delivery of Selenium Nanoparticles

This study aimed to design a hybrid oral liposomal delivery system for selenium nanoparticles (Lip-SeNPs) to improve the bioavailability of selenium. Thiolated chitosan, a multifunctional polymer with mucoadhesive properties, was used for surface functionalization of Lip-SeNPs. Selenium nanoparticle...

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Autores principales: Selmani, Atiđa, Seibert, Elisabeth, Tetyczka, Carolin, Kuehnelt, Doris, Vidakovic, Ivan, Kornmueller, Karin, Absenger-Novak, Markus, Radatović, Borna, Vinković Vrček, Ivana, Leitinger, Gerd, Fröhlich, Eleonore, Bernkop-Schnürch, Andreas, Roblegg, Eva, Prassl, Ruth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032237/
https://www.ncbi.nlm.nih.gov/pubmed/35456640
http://dx.doi.org/10.3390/pharmaceutics14040803
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author Selmani, Atiđa
Seibert, Elisabeth
Tetyczka, Carolin
Kuehnelt, Doris
Vidakovic, Ivan
Kornmueller, Karin
Absenger-Novak, Markus
Radatović, Borna
Vinković Vrček, Ivana
Leitinger, Gerd
Fröhlich, Eleonore
Bernkop-Schnürch, Andreas
Roblegg, Eva
Prassl, Ruth
author_facet Selmani, Atiđa
Seibert, Elisabeth
Tetyczka, Carolin
Kuehnelt, Doris
Vidakovic, Ivan
Kornmueller, Karin
Absenger-Novak, Markus
Radatović, Borna
Vinković Vrček, Ivana
Leitinger, Gerd
Fröhlich, Eleonore
Bernkop-Schnürch, Andreas
Roblegg, Eva
Prassl, Ruth
author_sort Selmani, Atiđa
collection PubMed
description This study aimed to design a hybrid oral liposomal delivery system for selenium nanoparticles (Lip-SeNPs) to improve the bioavailability of selenium. Thiolated chitosan, a multifunctional polymer with mucoadhesive properties, was used for surface functionalization of Lip-SeNPs. Selenium nanoparticle (SeNP)-loaded liposomes were manufactured by a single step microfluidics-assisted chemical reduction and assembling process. Subsequently, chitosan-N-acetylcysteine was covalently conjugated to the preformed Lip-SeNPs. The Lip-SeNPs were characterized in terms of composition, morphology, size, zeta potential, lipid organization, loading efficiency and radical scavenging activity. A co-culture system (Caco-2:HT29-MTX) that integrates mucus secreting and enterocyte-like cell types was used as a model of the human intestinal epithelium to determine adsorption, mucus penetration, release and transport properties of Lip-SeNPs in vitro. Thiolated Lip-SeNPs were positively charged with an average size of about 250 nm. Thiolated Lip-SeNPs tightly adhered to the mucus layer without penetrating the enterocytes. This finding was consistent with ex vivo adsorption studies using freshly excised porcine small intestinal tissues. Due to the improved mucoadhesion and retention in a simulated microenvironment of the small intestine, thiolated Lip-SeNPs might be a promising tool for oral selenium delivery.
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spelling pubmed-90322372022-04-23 Thiolated Chitosan Conjugated Liposomes for Oral Delivery of Selenium Nanoparticles Selmani, Atiđa Seibert, Elisabeth Tetyczka, Carolin Kuehnelt, Doris Vidakovic, Ivan Kornmueller, Karin Absenger-Novak, Markus Radatović, Borna Vinković Vrček, Ivana Leitinger, Gerd Fröhlich, Eleonore Bernkop-Schnürch, Andreas Roblegg, Eva Prassl, Ruth Pharmaceutics Article This study aimed to design a hybrid oral liposomal delivery system for selenium nanoparticles (Lip-SeNPs) to improve the bioavailability of selenium. Thiolated chitosan, a multifunctional polymer with mucoadhesive properties, was used for surface functionalization of Lip-SeNPs. Selenium nanoparticle (SeNP)-loaded liposomes were manufactured by a single step microfluidics-assisted chemical reduction and assembling process. Subsequently, chitosan-N-acetylcysteine was covalently conjugated to the preformed Lip-SeNPs. The Lip-SeNPs were characterized in terms of composition, morphology, size, zeta potential, lipid organization, loading efficiency and radical scavenging activity. A co-culture system (Caco-2:HT29-MTX) that integrates mucus secreting and enterocyte-like cell types was used as a model of the human intestinal epithelium to determine adsorption, mucus penetration, release and transport properties of Lip-SeNPs in vitro. Thiolated Lip-SeNPs were positively charged with an average size of about 250 nm. Thiolated Lip-SeNPs tightly adhered to the mucus layer without penetrating the enterocytes. This finding was consistent with ex vivo adsorption studies using freshly excised porcine small intestinal tissues. Due to the improved mucoadhesion and retention in a simulated microenvironment of the small intestine, thiolated Lip-SeNPs might be a promising tool for oral selenium delivery. MDPI 2022-04-06 /pmc/articles/PMC9032237/ /pubmed/35456640 http://dx.doi.org/10.3390/pharmaceutics14040803 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Selmani, Atiđa
Seibert, Elisabeth
Tetyczka, Carolin
Kuehnelt, Doris
Vidakovic, Ivan
Kornmueller, Karin
Absenger-Novak, Markus
Radatović, Borna
Vinković Vrček, Ivana
Leitinger, Gerd
Fröhlich, Eleonore
Bernkop-Schnürch, Andreas
Roblegg, Eva
Prassl, Ruth
Thiolated Chitosan Conjugated Liposomes for Oral Delivery of Selenium Nanoparticles
title Thiolated Chitosan Conjugated Liposomes for Oral Delivery of Selenium Nanoparticles
title_full Thiolated Chitosan Conjugated Liposomes for Oral Delivery of Selenium Nanoparticles
title_fullStr Thiolated Chitosan Conjugated Liposomes for Oral Delivery of Selenium Nanoparticles
title_full_unstemmed Thiolated Chitosan Conjugated Liposomes for Oral Delivery of Selenium Nanoparticles
title_short Thiolated Chitosan Conjugated Liposomes for Oral Delivery of Selenium Nanoparticles
title_sort thiolated chitosan conjugated liposomes for oral delivery of selenium nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032237/
https://www.ncbi.nlm.nih.gov/pubmed/35456640
http://dx.doi.org/10.3390/pharmaceutics14040803
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