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Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells

Background: Stem cells play an important role in the success of regenerative endodontic procedures. They are affected by the presence of medicaments that are used before the induction of bleeding or the creation of a scaffold for endodontic regeneration. This study examines the effects of different...

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Autores principales: Bhandi, Shilpa, Patil, Shankargouda, Boreak, Nezar, Chohan, Hitesh, AbuMelha, Abdulaziz S., Alkahtany, Mazen F., Almadi, Khalid H., Vinothkumar, Thilla Sekar, Raj, A. Thirumal, Testarelli, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032254/
https://www.ncbi.nlm.nih.gov/pubmed/35455691
http://dx.doi.org/10.3390/jpm12040575
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author Bhandi, Shilpa
Patil, Shankargouda
Boreak, Nezar
Chohan, Hitesh
AbuMelha, Abdulaziz S.
Alkahtany, Mazen F.
Almadi, Khalid H.
Vinothkumar, Thilla Sekar
Raj, A. Thirumal
Testarelli, Luca
author_facet Bhandi, Shilpa
Patil, Shankargouda
Boreak, Nezar
Chohan, Hitesh
AbuMelha, Abdulaziz S.
Alkahtany, Mazen F.
Almadi, Khalid H.
Vinothkumar, Thilla Sekar
Raj, A. Thirumal
Testarelli, Luca
author_sort Bhandi, Shilpa
collection PubMed
description Background: Stem cells play an important role in the success of regenerative endodontic procedures. They are affected by the presence of medicaments that are used before the induction of bleeding or the creation of a scaffold for endodontic regeneration. This study examines the effects of different intracanal medicaments on the viability and survival of dental pulp stem cells at different doses and over different exposure times. Methods: Dental pulp stem cells were cultured from healthy third molar teeth using the long-term explant culture method and characterized using flow cytometry and exposed to different concentrations of calcium hydroxide, doxycycline, potassium iodide, triamcinolone, and glutaraldehyde, each ranging from 0 (control) to 1000 µg/mL. Exposure times were 6, 24, and 48 h. Cell viability was measured using the MTT assay, and apoptosis was measured using the Annexin V-binding assay. Results: All medicaments significantly reduced cell viability at different concentrations over different exposure times. Calcium hydroxide and triamcinolone favored cell viability at higher concentrations during all exposure times compared to other medicaments. The apoptosis assay showed a significant increase in cell death on exposure to doxycycline, potassium iodide, and glutaraldehyde. Conclusion: The intracanal medicaments examined in our study affected the viability of dental pulp stem cells in a time and dose-dependent manner. They also adversely affected the survival of dental pulp stem cells. Further studies are needed to better understand the effect of prolonged exposure to medicaments according to clinical protocols and their effect on the stemness of dental pulp stem cells.
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spelling pubmed-90322542022-04-23 Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells Bhandi, Shilpa Patil, Shankargouda Boreak, Nezar Chohan, Hitesh AbuMelha, Abdulaziz S. Alkahtany, Mazen F. Almadi, Khalid H. Vinothkumar, Thilla Sekar Raj, A. Thirumal Testarelli, Luca J Pers Med Article Background: Stem cells play an important role in the success of regenerative endodontic procedures. They are affected by the presence of medicaments that are used before the induction of bleeding or the creation of a scaffold for endodontic regeneration. This study examines the effects of different intracanal medicaments on the viability and survival of dental pulp stem cells at different doses and over different exposure times. Methods: Dental pulp stem cells were cultured from healthy third molar teeth using the long-term explant culture method and characterized using flow cytometry and exposed to different concentrations of calcium hydroxide, doxycycline, potassium iodide, triamcinolone, and glutaraldehyde, each ranging from 0 (control) to 1000 µg/mL. Exposure times were 6, 24, and 48 h. Cell viability was measured using the MTT assay, and apoptosis was measured using the Annexin V-binding assay. Results: All medicaments significantly reduced cell viability at different concentrations over different exposure times. Calcium hydroxide and triamcinolone favored cell viability at higher concentrations during all exposure times compared to other medicaments. The apoptosis assay showed a significant increase in cell death on exposure to doxycycline, potassium iodide, and glutaraldehyde. Conclusion: The intracanal medicaments examined in our study affected the viability of dental pulp stem cells in a time and dose-dependent manner. They also adversely affected the survival of dental pulp stem cells. Further studies are needed to better understand the effect of prolonged exposure to medicaments according to clinical protocols and their effect on the stemness of dental pulp stem cells. MDPI 2022-04-04 /pmc/articles/PMC9032254/ /pubmed/35455691 http://dx.doi.org/10.3390/jpm12040575 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bhandi, Shilpa
Patil, Shankargouda
Boreak, Nezar
Chohan, Hitesh
AbuMelha, Abdulaziz S.
Alkahtany, Mazen F.
Almadi, Khalid H.
Vinothkumar, Thilla Sekar
Raj, A. Thirumal
Testarelli, Luca
Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells
title Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells
title_full Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells
title_fullStr Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells
title_full_unstemmed Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells
title_short Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells
title_sort effect of different intracanal medicaments on the viability and survival of dental pulp stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032254/
https://www.ncbi.nlm.nih.gov/pubmed/35455691
http://dx.doi.org/10.3390/jpm12040575
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