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Pathology and Molecular Epidemiology of Fowl Adenovirus Serotype 4 Outbreaks in Broiler Chicken in Abu Dhabi Emirate, UAE

Background: Fowl adenovirus serotype 4 (FAdV-4), causing inclusion body hepatitis (IBH) and hydropericardium hepatitis syndrome (HPS), is responsible for the significant economic losses in poultry industry worldwide. This study describes FAdV disease and molecular characteristics of the virus as the...

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Autores principales: Ishag, Hassan Zackaria Ali, Terab, Abdelnasir Mohammed Adam, El Tigani-Asil, El Tigani Ahmed, Bensalah, Oum Keltoum, Khalil, Nasereldien Altaib Hussein, Khalafalla, Abdelmalik Ibrahim, Al Hammadi, Zulaikha Mohamed Abdel Hameed, Shah, Asma Abdi Mohamed, Al Muhairi, Salama Suhail Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032256/
https://www.ncbi.nlm.nih.gov/pubmed/35448652
http://dx.doi.org/10.3390/vetsci9040154
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author Ishag, Hassan Zackaria Ali
Terab, Abdelnasir Mohammed Adam
El Tigani-Asil, El Tigani Ahmed
Bensalah, Oum Keltoum
Khalil, Nasereldien Altaib Hussein
Khalafalla, Abdelmalik Ibrahim
Al Hammadi, Zulaikha Mohamed Abdel Hameed
Shah, Asma Abdi Mohamed
Al Muhairi, Salama Suhail Mohammed
author_facet Ishag, Hassan Zackaria Ali
Terab, Abdelnasir Mohammed Adam
El Tigani-Asil, El Tigani Ahmed
Bensalah, Oum Keltoum
Khalil, Nasereldien Altaib Hussein
Khalafalla, Abdelmalik Ibrahim
Al Hammadi, Zulaikha Mohamed Abdel Hameed
Shah, Asma Abdi Mohamed
Al Muhairi, Salama Suhail Mohammed
author_sort Ishag, Hassan Zackaria Ali
collection PubMed
description Background: Fowl adenovirus serotype 4 (FAdV-4), causing inclusion body hepatitis (IBH) and hydropericardium hepatitis syndrome (HPS), is responsible for the significant economic losses in poultry industry worldwide. This study describes FAdV disease and molecular characteristics of the virus as the first report in UAE. Methodology: Clinical, necropsy, histopathology, qPCR and phylogenetic analysis of hexon gene were used to diagnose and characterize the virus. Results: The age of the infected broiler chicken was 2–4 weeks. The morbidity and mortality rates ranged between 50 and 100% and 44 and 100%, respectively. Clinically, sudden onset, diarrhea, anemia and general weakness were recorded. At necropsy, acute necrotic hepatitis, with swollen, yellowish discoloration, enlarged and friable liver; hydropericarditis with hydropericardium effusions; and enlarged mottled spleen were observed. Histopathology examination revealed degeneration and necrosis, lymphocytic infiltration and inclusion bodies. The qPCR analysis detected the virus in all samples tested. Hexon gene sequence analysis identified FAdV serotype 4, species C as the major cause of FAdV infections in UAE in 2020, and this strain was closely related to FAdV-4 circulating in Saudi Arabia, Pakistan, Nepal and China. Conclusion: The serotype 4, species C, was the common FAdV strain causing IBH and HPS episodes in the region. This result may help design effective vaccination programs that rely on field serotypes.
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spelling pubmed-90322562022-04-23 Pathology and Molecular Epidemiology of Fowl Adenovirus Serotype 4 Outbreaks in Broiler Chicken in Abu Dhabi Emirate, UAE Ishag, Hassan Zackaria Ali Terab, Abdelnasir Mohammed Adam El Tigani-Asil, El Tigani Ahmed Bensalah, Oum Keltoum Khalil, Nasereldien Altaib Hussein Khalafalla, Abdelmalik Ibrahim Al Hammadi, Zulaikha Mohamed Abdel Hameed Shah, Asma Abdi Mohamed Al Muhairi, Salama Suhail Mohammed Vet Sci Article Background: Fowl adenovirus serotype 4 (FAdV-4), causing inclusion body hepatitis (IBH) and hydropericardium hepatitis syndrome (HPS), is responsible for the significant economic losses in poultry industry worldwide. This study describes FAdV disease and molecular characteristics of the virus as the first report in UAE. Methodology: Clinical, necropsy, histopathology, qPCR and phylogenetic analysis of hexon gene were used to diagnose and characterize the virus. Results: The age of the infected broiler chicken was 2–4 weeks. The morbidity and mortality rates ranged between 50 and 100% and 44 and 100%, respectively. Clinically, sudden onset, diarrhea, anemia and general weakness were recorded. At necropsy, acute necrotic hepatitis, with swollen, yellowish discoloration, enlarged and friable liver; hydropericarditis with hydropericardium effusions; and enlarged mottled spleen were observed. Histopathology examination revealed degeneration and necrosis, lymphocytic infiltration and inclusion bodies. The qPCR analysis detected the virus in all samples tested. Hexon gene sequence analysis identified FAdV serotype 4, species C as the major cause of FAdV infections in UAE in 2020, and this strain was closely related to FAdV-4 circulating in Saudi Arabia, Pakistan, Nepal and China. Conclusion: The serotype 4, species C, was the common FAdV strain causing IBH and HPS episodes in the region. This result may help design effective vaccination programs that rely on field serotypes. MDPI 2022-03-23 /pmc/articles/PMC9032256/ /pubmed/35448652 http://dx.doi.org/10.3390/vetsci9040154 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ishag, Hassan Zackaria Ali
Terab, Abdelnasir Mohammed Adam
El Tigani-Asil, El Tigani Ahmed
Bensalah, Oum Keltoum
Khalil, Nasereldien Altaib Hussein
Khalafalla, Abdelmalik Ibrahim
Al Hammadi, Zulaikha Mohamed Abdel Hameed
Shah, Asma Abdi Mohamed
Al Muhairi, Salama Suhail Mohammed
Pathology and Molecular Epidemiology of Fowl Adenovirus Serotype 4 Outbreaks in Broiler Chicken in Abu Dhabi Emirate, UAE
title Pathology and Molecular Epidemiology of Fowl Adenovirus Serotype 4 Outbreaks in Broiler Chicken in Abu Dhabi Emirate, UAE
title_full Pathology and Molecular Epidemiology of Fowl Adenovirus Serotype 4 Outbreaks in Broiler Chicken in Abu Dhabi Emirate, UAE
title_fullStr Pathology and Molecular Epidemiology of Fowl Adenovirus Serotype 4 Outbreaks in Broiler Chicken in Abu Dhabi Emirate, UAE
title_full_unstemmed Pathology and Molecular Epidemiology of Fowl Adenovirus Serotype 4 Outbreaks in Broiler Chicken in Abu Dhabi Emirate, UAE
title_short Pathology and Molecular Epidemiology of Fowl Adenovirus Serotype 4 Outbreaks in Broiler Chicken in Abu Dhabi Emirate, UAE
title_sort pathology and molecular epidemiology of fowl adenovirus serotype 4 outbreaks in broiler chicken in abu dhabi emirate, uae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032256/
https://www.ncbi.nlm.nih.gov/pubmed/35448652
http://dx.doi.org/10.3390/vetsci9040154
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