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Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria

Ribosome biogenesis is a fundamental and multistage process. The basic steps of ribosome assembly are the transcription, processing, folding, and modification of rRNA; the translation, folding, and modification of r-proteins; and consecutive binding of ribosomal proteins to rRNAs. Ribosome maturatio...

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Autores principales: Maksimova, Elena, Kravchenko, Olesya, Korepanov, Alexey, Stolboushkina, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032327/
https://www.ncbi.nlm.nih.gov/pubmed/35456798
http://dx.doi.org/10.3390/microorganisms10040747
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author Maksimova, Elena
Kravchenko, Olesya
Korepanov, Alexey
Stolboushkina, Elena
author_facet Maksimova, Elena
Kravchenko, Olesya
Korepanov, Alexey
Stolboushkina, Elena
author_sort Maksimova, Elena
collection PubMed
description Ribosome biogenesis is a fundamental and multistage process. The basic steps of ribosome assembly are the transcription, processing, folding, and modification of rRNA; the translation, folding, and modification of r-proteins; and consecutive binding of ribosomal proteins to rRNAs. Ribosome maturation is facilitated by biogenesis factors that include a broad spectrum of proteins: GTPases, RNA helicases, endonucleases, modification enzymes, molecular chaperones, etc. The ribosome assembly factors assist proper rRNA folding and protein–RNA interactions and may sense the checkpoints during the assembly to ensure correct order of this process. Inactivation of these factors is accompanied by severe growth phenotypes and accumulation of immature ribosomal subunits containing unprocessed rRNA, which reduces overall translation efficiency and causes translational errors. In this review, we focus on the structural and biochemical analysis of the 30S ribosomal subunit assembly factors RbfA, YjeQ (RsgA), Era, KsgA (RsmA), RimJ, RimM, RimP, and Hfq, which take part in the decoding-center folding.
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spelling pubmed-90323272022-04-23 Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria Maksimova, Elena Kravchenko, Olesya Korepanov, Alexey Stolboushkina, Elena Microorganisms Review Ribosome biogenesis is a fundamental and multistage process. The basic steps of ribosome assembly are the transcription, processing, folding, and modification of rRNA; the translation, folding, and modification of r-proteins; and consecutive binding of ribosomal proteins to rRNAs. Ribosome maturation is facilitated by biogenesis factors that include a broad spectrum of proteins: GTPases, RNA helicases, endonucleases, modification enzymes, molecular chaperones, etc. The ribosome assembly factors assist proper rRNA folding and protein–RNA interactions and may sense the checkpoints during the assembly to ensure correct order of this process. Inactivation of these factors is accompanied by severe growth phenotypes and accumulation of immature ribosomal subunits containing unprocessed rRNA, which reduces overall translation efficiency and causes translational errors. In this review, we focus on the structural and biochemical analysis of the 30S ribosomal subunit assembly factors RbfA, YjeQ (RsgA), Era, KsgA (RsmA), RimJ, RimM, RimP, and Hfq, which take part in the decoding-center folding. MDPI 2022-03-30 /pmc/articles/PMC9032327/ /pubmed/35456798 http://dx.doi.org/10.3390/microorganisms10040747 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Maksimova, Elena
Kravchenko, Olesya
Korepanov, Alexey
Stolboushkina, Elena
Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria
title Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria
title_full Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria
title_fullStr Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria
title_full_unstemmed Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria
title_short Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria
title_sort protein assistants of small ribosomal subunit biogenesis in bacteria
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032327/
https://www.ncbi.nlm.nih.gov/pubmed/35456798
http://dx.doi.org/10.3390/microorganisms10040747
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