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Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA

The presence of neutralizing antibodies (NAbs) against SARS-CoV-2 represent a surrogate marker of immunologic protection in populations at high risk of infection such as healthcare workers caring for hospitalized patients with COVID-19. As recommended by CDC and the European CDC, the use of rapid di...

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Autores principales: Tuells, José, Parra-Grande, Mónica, Santos-Calle, Francisco J., Montagud, Ana C., Egoavil, Cecilia M., García-Rivera, Celia, Caballero, Pablo, Gabaldón-Bravo, Eva M., Rodríguez-Diaz, Juan Carlos, Hurtado-Sánchez, José Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032347/
https://www.ncbi.nlm.nih.gov/pubmed/35455259
http://dx.doi.org/10.3390/vaccines10040510
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author Tuells, José
Parra-Grande, Mónica
Santos-Calle, Francisco J.
Montagud, Ana C.
Egoavil, Cecilia M.
García-Rivera, Celia
Caballero, Pablo
Gabaldón-Bravo, Eva M.
Rodríguez-Diaz, Juan Carlos
Hurtado-Sánchez, José Antonio
author_facet Tuells, José
Parra-Grande, Mónica
Santos-Calle, Francisco J.
Montagud, Ana C.
Egoavil, Cecilia M.
García-Rivera, Celia
Caballero, Pablo
Gabaldón-Bravo, Eva M.
Rodríguez-Diaz, Juan Carlos
Hurtado-Sánchez, José Antonio
author_sort Tuells, José
collection PubMed
description The presence of neutralizing antibodies (NAbs) against SARS-CoV-2 represent a surrogate marker of immunologic protection in populations at high risk of infection such as healthcare workers caring for hospitalized patients with COVID-19. As recommended by CDC and the European CDC, the use of rapid diagnostic tests during population-based evaluations offers an opportunity to identify individuals with serologic evidence of natural infection or who have undergone vaccination. We carried out a cross-sectional study to assess the presence of neutralizing antibodies against SARS-CoV-2 among medical providers at an intensive care unit of a large referral hospital in Alicante, Spain. In addition, we tested for the presence of neutralizing antibodies compared to serum of uninfected individuals from a Biobank. We were also interested in evaluating the use of a rapid lateral flow immunochromatography (LFIC) test against a surrogate ELISA viral neutralization test (sVNT). This rapid test demonstrated a specificity of 1.000 95% CI (0.91–1.00) and the sensitivity of 0.987 95% CI (0.93–1.00). The negative predictive value was 95%. After six months, this rapid test demonstrated that those immunized with two doses of BioNTech/Pfizer vaccine, maintained optimal levels of neutralizing antibodies. We concluded that all Health Care Workers develop NAbs and the use of this rapid immunochromatographic test represents a potential tool to be used in population-based studies to detect serological antibody responses to vaccination. Vaccination policies could benefit from this tool to assess additional doses of vaccine or boosters among high-risk populations.
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spelling pubmed-90323472022-04-23 Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA Tuells, José Parra-Grande, Mónica Santos-Calle, Francisco J. Montagud, Ana C. Egoavil, Cecilia M. García-Rivera, Celia Caballero, Pablo Gabaldón-Bravo, Eva M. Rodríguez-Diaz, Juan Carlos Hurtado-Sánchez, José Antonio Vaccines (Basel) Article The presence of neutralizing antibodies (NAbs) against SARS-CoV-2 represent a surrogate marker of immunologic protection in populations at high risk of infection such as healthcare workers caring for hospitalized patients with COVID-19. As recommended by CDC and the European CDC, the use of rapid diagnostic tests during population-based evaluations offers an opportunity to identify individuals with serologic evidence of natural infection or who have undergone vaccination. We carried out a cross-sectional study to assess the presence of neutralizing antibodies against SARS-CoV-2 among medical providers at an intensive care unit of a large referral hospital in Alicante, Spain. In addition, we tested for the presence of neutralizing antibodies compared to serum of uninfected individuals from a Biobank. We were also interested in evaluating the use of a rapid lateral flow immunochromatography (LFIC) test against a surrogate ELISA viral neutralization test (sVNT). This rapid test demonstrated a specificity of 1.000 95% CI (0.91–1.00) and the sensitivity of 0.987 95% CI (0.93–1.00). The negative predictive value was 95%. After six months, this rapid test demonstrated that those immunized with two doses of BioNTech/Pfizer vaccine, maintained optimal levels of neutralizing antibodies. We concluded that all Health Care Workers develop NAbs and the use of this rapid immunochromatographic test represents a potential tool to be used in population-based studies to detect serological antibody responses to vaccination. Vaccination policies could benefit from this tool to assess additional doses of vaccine or boosters among high-risk populations. MDPI 2022-03-25 /pmc/articles/PMC9032347/ /pubmed/35455259 http://dx.doi.org/10.3390/vaccines10040510 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tuells, José
Parra-Grande, Mónica
Santos-Calle, Francisco J.
Montagud, Ana C.
Egoavil, Cecilia M.
García-Rivera, Celia
Caballero, Pablo
Gabaldón-Bravo, Eva M.
Rodríguez-Diaz, Juan Carlos
Hurtado-Sánchez, José Antonio
Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA
title Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA
title_full Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA
title_fullStr Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA
title_full_unstemmed Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA
title_short Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA
title_sort detection of neutralizing antibodies against sars-cov-2 post-vaccination in health care workers of a large tertiary hospital in spain by using a rapid test lfic and svnt-elisa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032347/
https://www.ncbi.nlm.nih.gov/pubmed/35455259
http://dx.doi.org/10.3390/vaccines10040510
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