Cargando…

Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling

Primary cutaneous large B-cell lymphomas (PCLBCL) represent a diagnostic challenge because they are classified as PCLBCL, leg type (PCLBCL, LT) or primary cutaneous follicle centre lymphoma, large cell (PCFCL, LC), which differ by prognosis and therapeutic requirement. Unclassified cases with discor...

Descripción completa

Detalles Bibliográficos
Autores principales: Gros, Audrey, Menguy, Sarah, Bobée, Victor, Ducharme, Océane, Cassaigne, Isabelle Cirilo, Vergier, Béatrice, Parrens, Marie, Beylot-Barry, Marie, Pham-Ledard, Anne, Ruminy, Philippe, Jardin, Fabrice, Merlio, Jean-Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032422/
https://www.ncbi.nlm.nih.gov/pubmed/35452489
http://dx.doi.org/10.1371/journal.pone.0266978
_version_ 1784692640171687936
author Gros, Audrey
Menguy, Sarah
Bobée, Victor
Ducharme, Océane
Cassaigne, Isabelle Cirilo
Vergier, Béatrice
Parrens, Marie
Beylot-Barry, Marie
Pham-Ledard, Anne
Ruminy, Philippe
Jardin, Fabrice
Merlio, Jean-Philippe
author_facet Gros, Audrey
Menguy, Sarah
Bobée, Victor
Ducharme, Océane
Cassaigne, Isabelle Cirilo
Vergier, Béatrice
Parrens, Marie
Beylot-Barry, Marie
Pham-Ledard, Anne
Ruminy, Philippe
Jardin, Fabrice
Merlio, Jean-Philippe
author_sort Gros, Audrey
collection PubMed
description Primary cutaneous large B-cell lymphomas (PCLBCL) represent a diagnostic challenge because they are classified as PCLBCL, leg type (PCLBCL, LT) or primary cutaneous follicle centre lymphoma, large cell (PCFCL, LC), which differ by prognosis and therapeutic requirement. Unclassified cases with discordant clinical presentations, morphologies, and immunophenotypes may be classified into the not otherwise specified (PCLBCL, NOS) category based on ancillary molecular analyses. Cell-of-origin profiling as germinal centre (GC) type or non-GC type by immunohistochemistry is not considered reproducible because of variable CD10 expression. In a series of 55 PCLBCL cases with > 80% large cells, we reported 21 PCFCL, LC cases as GC-type and 27 PCLBCL, LT as non-GC-type; 7 cases were considered PCLBCL, NOS. Here, we demonstrate the accuracy of molecular profiling of PCLBCL as GC or non-GC type using a reverse transcriptase multiplex ligation assay (RT-MLPA). RT-MLPA classified the seven PCLBCL, NOS cases in accordance with their mutational profile. An integrative principal component analysis confirmed the main criteria and the relevance of genomic profiling of PCFCL, LC as GC-derived, and PCLBCL, LT as non-GC-derived. Both the cell-of-origin classification of PCLBCL and the integrative analysis identified two clinically relevant subgroups according to overall survival, which may help to standardize PCLBCL diagnosis and patient management.
format Online
Article
Text
id pubmed-9032422
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-90324222022-04-23 Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling Gros, Audrey Menguy, Sarah Bobée, Victor Ducharme, Océane Cassaigne, Isabelle Cirilo Vergier, Béatrice Parrens, Marie Beylot-Barry, Marie Pham-Ledard, Anne Ruminy, Philippe Jardin, Fabrice Merlio, Jean-Philippe PLoS One Research Article Primary cutaneous large B-cell lymphomas (PCLBCL) represent a diagnostic challenge because they are classified as PCLBCL, leg type (PCLBCL, LT) or primary cutaneous follicle centre lymphoma, large cell (PCFCL, LC), which differ by prognosis and therapeutic requirement. Unclassified cases with discordant clinical presentations, morphologies, and immunophenotypes may be classified into the not otherwise specified (PCLBCL, NOS) category based on ancillary molecular analyses. Cell-of-origin profiling as germinal centre (GC) type or non-GC type by immunohistochemistry is not considered reproducible because of variable CD10 expression. In a series of 55 PCLBCL cases with > 80% large cells, we reported 21 PCFCL, LC cases as GC-type and 27 PCLBCL, LT as non-GC-type; 7 cases were considered PCLBCL, NOS. Here, we demonstrate the accuracy of molecular profiling of PCLBCL as GC or non-GC type using a reverse transcriptase multiplex ligation assay (RT-MLPA). RT-MLPA classified the seven PCLBCL, NOS cases in accordance with their mutational profile. An integrative principal component analysis confirmed the main criteria and the relevance of genomic profiling of PCFCL, LC as GC-derived, and PCLBCL, LT as non-GC-derived. Both the cell-of-origin classification of PCLBCL and the integrative analysis identified two clinically relevant subgroups according to overall survival, which may help to standardize PCLBCL diagnosis and patient management. Public Library of Science 2022-04-22 /pmc/articles/PMC9032422/ /pubmed/35452489 http://dx.doi.org/10.1371/journal.pone.0266978 Text en © 2022 Gros et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gros, Audrey
Menguy, Sarah
Bobée, Victor
Ducharme, Océane
Cassaigne, Isabelle Cirilo
Vergier, Béatrice
Parrens, Marie
Beylot-Barry, Marie
Pham-Ledard, Anne
Ruminy, Philippe
Jardin, Fabrice
Merlio, Jean-Philippe
Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling
title Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling
title_full Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling
title_fullStr Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling
title_full_unstemmed Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling
title_short Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling
title_sort integrative diagnosis of primary cutaneous large b-cell lymphomas supports the relevance of cell of origin profiling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032422/
https://www.ncbi.nlm.nih.gov/pubmed/35452489
http://dx.doi.org/10.1371/journal.pone.0266978
work_keys_str_mv AT grosaudrey integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT menguysarah integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT bobeevictor integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT ducharmeoceane integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT cassaigneisabellecirilo integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT vergierbeatrice integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT parrensmarie integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT beylotbarrymarie integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT phamledardanne integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT ruminyphilippe integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT jardinfabrice integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling
AT merliojeanphilippe integrativediagnosisofprimarycutaneouslargebcelllymphomassupportstherelevanceofcelloforiginprofiling