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Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling
Primary cutaneous large B-cell lymphomas (PCLBCL) represent a diagnostic challenge because they are classified as PCLBCL, leg type (PCLBCL, LT) or primary cutaneous follicle centre lymphoma, large cell (PCFCL, LC), which differ by prognosis and therapeutic requirement. Unclassified cases with discor...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032422/ https://www.ncbi.nlm.nih.gov/pubmed/35452489 http://dx.doi.org/10.1371/journal.pone.0266978 |
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author | Gros, Audrey Menguy, Sarah Bobée, Victor Ducharme, Océane Cassaigne, Isabelle Cirilo Vergier, Béatrice Parrens, Marie Beylot-Barry, Marie Pham-Ledard, Anne Ruminy, Philippe Jardin, Fabrice Merlio, Jean-Philippe |
author_facet | Gros, Audrey Menguy, Sarah Bobée, Victor Ducharme, Océane Cassaigne, Isabelle Cirilo Vergier, Béatrice Parrens, Marie Beylot-Barry, Marie Pham-Ledard, Anne Ruminy, Philippe Jardin, Fabrice Merlio, Jean-Philippe |
author_sort | Gros, Audrey |
collection | PubMed |
description | Primary cutaneous large B-cell lymphomas (PCLBCL) represent a diagnostic challenge because they are classified as PCLBCL, leg type (PCLBCL, LT) or primary cutaneous follicle centre lymphoma, large cell (PCFCL, LC), which differ by prognosis and therapeutic requirement. Unclassified cases with discordant clinical presentations, morphologies, and immunophenotypes may be classified into the not otherwise specified (PCLBCL, NOS) category based on ancillary molecular analyses. Cell-of-origin profiling as germinal centre (GC) type or non-GC type by immunohistochemistry is not considered reproducible because of variable CD10 expression. In a series of 55 PCLBCL cases with > 80% large cells, we reported 21 PCFCL, LC cases as GC-type and 27 PCLBCL, LT as non-GC-type; 7 cases were considered PCLBCL, NOS. Here, we demonstrate the accuracy of molecular profiling of PCLBCL as GC or non-GC type using a reverse transcriptase multiplex ligation assay (RT-MLPA). RT-MLPA classified the seven PCLBCL, NOS cases in accordance with their mutational profile. An integrative principal component analysis confirmed the main criteria and the relevance of genomic profiling of PCFCL, LC as GC-derived, and PCLBCL, LT as non-GC-derived. Both the cell-of-origin classification of PCLBCL and the integrative analysis identified two clinically relevant subgroups according to overall survival, which may help to standardize PCLBCL diagnosis and patient management. |
format | Online Article Text |
id | pubmed-9032422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-90324222022-04-23 Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling Gros, Audrey Menguy, Sarah Bobée, Victor Ducharme, Océane Cassaigne, Isabelle Cirilo Vergier, Béatrice Parrens, Marie Beylot-Barry, Marie Pham-Ledard, Anne Ruminy, Philippe Jardin, Fabrice Merlio, Jean-Philippe PLoS One Research Article Primary cutaneous large B-cell lymphomas (PCLBCL) represent a diagnostic challenge because they are classified as PCLBCL, leg type (PCLBCL, LT) or primary cutaneous follicle centre lymphoma, large cell (PCFCL, LC), which differ by prognosis and therapeutic requirement. Unclassified cases with discordant clinical presentations, morphologies, and immunophenotypes may be classified into the not otherwise specified (PCLBCL, NOS) category based on ancillary molecular analyses. Cell-of-origin profiling as germinal centre (GC) type or non-GC type by immunohistochemistry is not considered reproducible because of variable CD10 expression. In a series of 55 PCLBCL cases with > 80% large cells, we reported 21 PCFCL, LC cases as GC-type and 27 PCLBCL, LT as non-GC-type; 7 cases were considered PCLBCL, NOS. Here, we demonstrate the accuracy of molecular profiling of PCLBCL as GC or non-GC type using a reverse transcriptase multiplex ligation assay (RT-MLPA). RT-MLPA classified the seven PCLBCL, NOS cases in accordance with their mutational profile. An integrative principal component analysis confirmed the main criteria and the relevance of genomic profiling of PCFCL, LC as GC-derived, and PCLBCL, LT as non-GC-derived. Both the cell-of-origin classification of PCLBCL and the integrative analysis identified two clinically relevant subgroups according to overall survival, which may help to standardize PCLBCL diagnosis and patient management. Public Library of Science 2022-04-22 /pmc/articles/PMC9032422/ /pubmed/35452489 http://dx.doi.org/10.1371/journal.pone.0266978 Text en © 2022 Gros et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gros, Audrey Menguy, Sarah Bobée, Victor Ducharme, Océane Cassaigne, Isabelle Cirilo Vergier, Béatrice Parrens, Marie Beylot-Barry, Marie Pham-Ledard, Anne Ruminy, Philippe Jardin, Fabrice Merlio, Jean-Philippe Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling |
title | Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling |
title_full | Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling |
title_fullStr | Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling |
title_full_unstemmed | Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling |
title_short | Integrative diagnosis of primary cutaneous large B-cell lymphomas supports the relevance of cell of origin profiling |
title_sort | integrative diagnosis of primary cutaneous large b-cell lymphomas supports the relevance of cell of origin profiling |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032422/ https://www.ncbi.nlm.nih.gov/pubmed/35452489 http://dx.doi.org/10.1371/journal.pone.0266978 |
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