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Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization

Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterologous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal b...

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Autores principales: Shenouda, Mary L., Ambilika, Maria, Skellam, Elizabeth, Cox, Russell J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032437/
https://www.ncbi.nlm.nih.gov/pubmed/35448586
http://dx.doi.org/10.3390/jof8040355
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author Shenouda, Mary L.
Ambilika, Maria
Skellam, Elizabeth
Cox, Russell J.
author_facet Shenouda, Mary L.
Ambilika, Maria
Skellam, Elizabeth
Cox, Russell J.
author_sort Shenouda, Mary L.
collection PubMed
description Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterologous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.
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spelling pubmed-90324372022-04-23 Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization Shenouda, Mary L. Ambilika, Maria Skellam, Elizabeth Cox, Russell J. J Fungi (Basel) Article Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterologous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites. MDPI 2022-03-30 /pmc/articles/PMC9032437/ /pubmed/35448586 http://dx.doi.org/10.3390/jof8040355 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shenouda, Mary L.
Ambilika, Maria
Skellam, Elizabeth
Cox, Russell J.
Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization
title Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization
title_full Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization
title_fullStr Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization
title_full_unstemmed Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization
title_short Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization
title_sort heterologous expression of secondary metabolite genes in trichoderma reesei for waste valorization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032437/
https://www.ncbi.nlm.nih.gov/pubmed/35448586
http://dx.doi.org/10.3390/jof8040355
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