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Neuronal Electrophysiological Activities Detection of Defense Behaviors Using an Implantable Microelectrode Array in the Dorsal Periaqueductal Gray

Defense is the basic survival mechanism of animals when facing dangers. Previous studies have shown that the midbrain periaqueduct gray (PAG) was essential for the production of defense responses. However, the correlation between the endogenous neuronal activities of the dorsal PAG (dPAG) and differ...

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Autores principales: Lu, Botao, Fan, Penghui, Wang, Yiding, Dai, Yuchuan, Xie, Jingyu, Yang, Gucheng, Mo, Fan, Xu, Zhaojie, Song, Yilin, Liu, Juntao, Cai, Xinxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032743/
https://www.ncbi.nlm.nih.gov/pubmed/35448253
http://dx.doi.org/10.3390/bios12040193
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author Lu, Botao
Fan, Penghui
Wang, Yiding
Dai, Yuchuan
Xie, Jingyu
Yang, Gucheng
Mo, Fan
Xu, Zhaojie
Song, Yilin
Liu, Juntao
Cai, Xinxia
author_facet Lu, Botao
Fan, Penghui
Wang, Yiding
Dai, Yuchuan
Xie, Jingyu
Yang, Gucheng
Mo, Fan
Xu, Zhaojie
Song, Yilin
Liu, Juntao
Cai, Xinxia
author_sort Lu, Botao
collection PubMed
description Defense is the basic survival mechanism of animals when facing dangers. Previous studies have shown that the midbrain periaqueduct gray (PAG) was essential for the production of defense responses. However, the correlation between the endogenous neuronal activities of the dorsal PAG (dPAG) and different defense behaviors was still unclear. In this article, we designed and manufactured microelectrode arrays (MEAs) whose detection sites were arranged to match the shape and position of dPAG in rats, and modified it with platinum-black nanoparticles to improve the detection performance. Subsequently, we successfully recorded the electrophysiological activities of dPAG neurons via designed MEAs in freely behaving rats before and after exposure to the potent analog of predator odor 2-methyl-2-thiazoline (2-MT). Results demonstrated that 2-MT could cause strong innate fear and a series of defensive behaviors, accompanied by the significantly increased average firing rate and local field potential (LFP) power of neurons in dPAG. We also observed that dPAG participated in different defense behaviors with different degrees of activation, which was significantly stronger in the flight stage. Further analysis showed that the neuronal activities of dPAG neurons were earlier than flight, and the intensity of activation was inversely proportional to the distance from predator odor. Overall, our results indicate that dPAG neuronal activities play a crucial role in controlling different types of predator odor-evoked innate fear/defensive behaviors, and provide some guidance for the prediction of defense behavior.
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spelling pubmed-90327432022-04-23 Neuronal Electrophysiological Activities Detection of Defense Behaviors Using an Implantable Microelectrode Array in the Dorsal Periaqueductal Gray Lu, Botao Fan, Penghui Wang, Yiding Dai, Yuchuan Xie, Jingyu Yang, Gucheng Mo, Fan Xu, Zhaojie Song, Yilin Liu, Juntao Cai, Xinxia Biosensors (Basel) Article Defense is the basic survival mechanism of animals when facing dangers. Previous studies have shown that the midbrain periaqueduct gray (PAG) was essential for the production of defense responses. However, the correlation between the endogenous neuronal activities of the dorsal PAG (dPAG) and different defense behaviors was still unclear. In this article, we designed and manufactured microelectrode arrays (MEAs) whose detection sites were arranged to match the shape and position of dPAG in rats, and modified it with platinum-black nanoparticles to improve the detection performance. Subsequently, we successfully recorded the electrophysiological activities of dPAG neurons via designed MEAs in freely behaving rats before and after exposure to the potent analog of predator odor 2-methyl-2-thiazoline (2-MT). Results demonstrated that 2-MT could cause strong innate fear and a series of defensive behaviors, accompanied by the significantly increased average firing rate and local field potential (LFP) power of neurons in dPAG. We also observed that dPAG participated in different defense behaviors with different degrees of activation, which was significantly stronger in the flight stage. Further analysis showed that the neuronal activities of dPAG neurons were earlier than flight, and the intensity of activation was inversely proportional to the distance from predator odor. Overall, our results indicate that dPAG neuronal activities play a crucial role in controlling different types of predator odor-evoked innate fear/defensive behaviors, and provide some guidance for the prediction of defense behavior. MDPI 2022-03-25 /pmc/articles/PMC9032743/ /pubmed/35448253 http://dx.doi.org/10.3390/bios12040193 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Botao
Fan, Penghui
Wang, Yiding
Dai, Yuchuan
Xie, Jingyu
Yang, Gucheng
Mo, Fan
Xu, Zhaojie
Song, Yilin
Liu, Juntao
Cai, Xinxia
Neuronal Electrophysiological Activities Detection of Defense Behaviors Using an Implantable Microelectrode Array in the Dorsal Periaqueductal Gray
title Neuronal Electrophysiological Activities Detection of Defense Behaviors Using an Implantable Microelectrode Array in the Dorsal Periaqueductal Gray
title_full Neuronal Electrophysiological Activities Detection of Defense Behaviors Using an Implantable Microelectrode Array in the Dorsal Periaqueductal Gray
title_fullStr Neuronal Electrophysiological Activities Detection of Defense Behaviors Using an Implantable Microelectrode Array in the Dorsal Periaqueductal Gray
title_full_unstemmed Neuronal Electrophysiological Activities Detection of Defense Behaviors Using an Implantable Microelectrode Array in the Dorsal Periaqueductal Gray
title_short Neuronal Electrophysiological Activities Detection of Defense Behaviors Using an Implantable Microelectrode Array in the Dorsal Periaqueductal Gray
title_sort neuronal electrophysiological activities detection of defense behaviors using an implantable microelectrode array in the dorsal periaqueductal gray
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032743/
https://www.ncbi.nlm.nih.gov/pubmed/35448253
http://dx.doi.org/10.3390/bios12040193
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