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Multi-Omics Approach Points to the Importance of Oxylipins Metabolism in Early-Stage Breast Cancer

SIMPLE SUMMARY: On a system level, multi-omics approaches allow studying oxylipins, metabolites of omega-3 or omega-6 polyunsaturated fatty acids. We compared the ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) oxylipin profile signatures in the blood plasma of 152 health...

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Autores principales: Chistyakov, Dmitry V., Guryleva, Mariia V., Stepanova, Elena S., Makarenkova, Lyubov M., Ptitsyna, Elena V., Goriainov, Sergei V., Nikolskaya, Arina I., Astakhova, Alina A., Klimenko, Anna S., Bezborodova, Olga A., Rasskazova, Elena A., Potanina, Olga G., Abramovich, Rimma A., Nemtsova, Elena R., Sergeeva, Marina G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032865/
https://www.ncbi.nlm.nih.gov/pubmed/35454947
http://dx.doi.org/10.3390/cancers14082041
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author Chistyakov, Dmitry V.
Guryleva, Mariia V.
Stepanova, Elena S.
Makarenkova, Lyubov M.
Ptitsyna, Elena V.
Goriainov, Sergei V.
Nikolskaya, Arina I.
Astakhova, Alina A.
Klimenko, Anna S.
Bezborodova, Olga A.
Rasskazova, Elena A.
Potanina, Olga G.
Abramovich, Rimma A.
Nemtsova, Elena R.
Sergeeva, Marina G.
author_facet Chistyakov, Dmitry V.
Guryleva, Mariia V.
Stepanova, Elena S.
Makarenkova, Lyubov M.
Ptitsyna, Elena V.
Goriainov, Sergei V.
Nikolskaya, Arina I.
Astakhova, Alina A.
Klimenko, Anna S.
Bezborodova, Olga A.
Rasskazova, Elena A.
Potanina, Olga G.
Abramovich, Rimma A.
Nemtsova, Elena R.
Sergeeva, Marina G.
author_sort Chistyakov, Dmitry V.
collection PubMed
description SIMPLE SUMMARY: On a system level, multi-omics approaches allow studying oxylipins, metabolites of omega-3 or omega-6 polyunsaturated fatty acids. We compared the ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) oxylipin profile signatures in the blood plasma of 152 healthy volunteers (HC) and 169 patients with different stages of breast cancer (BC). We identified 18 differentially expressed oxylipins between BC vs. HC patients, including anandamide, prostaglandins, and hydroxydocosahexaenoic acids. To integrate lipidomics, transcriptomics, and genomics data, we analyzed a transcriptome of 10 open database datasets obtained from tissues and blood cells of BC patients and SNP data for 33 genes related to oxylipin metabolism and revealed 19 changed genes, among them CYP2C19, PTGS2, HPGD, and FAAH included in the list of DEGs in the analysis of transcriptomes and the list of SNPs associated with BC. Our data allow us to suppose that oxylipin profiles can be used to evaluate the early stages of breast cancer. ABSTRACT: The involvement of oxylipins, metabolites of polyunsaturated fatty acids, in cancer pathogenesis was known long ago, but only the development of the high-throughput methods get the opportunity to study oxylipins on a system level. The study aimed to elucidate alterations in oxylipin metabolism as characteristics of breast cancer patients. We compared the ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) oxylipin profile signatures in the blood plasma of 152 healthy volunteers (HC) and 169 patients with different stages of breast cancer (BC). To integrate lipidomics, transcriptomics, and genomics data, we analyzed a transcriptome of 10 open database datasets obtained from tissues and blood cells of BC patients and SNP data for 33 genes related to oxylipin metabolism. We identified 18 oxylipins, metabolites of omega-3 or omega-6 polyunsaturated fatty acids, that were differentially expressed between BCvsHC patients, including anandamide, prostaglandins and hydroxydocosahexaenoic acids. DEGs analysis of tissue and blood samples from BC patients revealed that 19 genes for oxylipin biosynthesis change their expression level, with CYP2C19, PTGS2, HPGD, and FAAH included in the list of DEGs in the analysis of transcriptomes and the list of SNPs associated with BC. Results allow us to suppose that oxylipin signatures reflect the organism’s level of response to the disease. Our data regarding changes in oxylipins at the system level show that oxylipin profiles can be used to evaluate the early stages of breast cancer.
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spelling pubmed-90328652022-04-23 Multi-Omics Approach Points to the Importance of Oxylipins Metabolism in Early-Stage Breast Cancer Chistyakov, Dmitry V. Guryleva, Mariia V. Stepanova, Elena S. Makarenkova, Lyubov M. Ptitsyna, Elena V. Goriainov, Sergei V. Nikolskaya, Arina I. Astakhova, Alina A. Klimenko, Anna S. Bezborodova, Olga A. Rasskazova, Elena A. Potanina, Olga G. Abramovich, Rimma A. Nemtsova, Elena R. Sergeeva, Marina G. Cancers (Basel) Article SIMPLE SUMMARY: On a system level, multi-omics approaches allow studying oxylipins, metabolites of omega-3 or omega-6 polyunsaturated fatty acids. We compared the ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) oxylipin profile signatures in the blood plasma of 152 healthy volunteers (HC) and 169 patients with different stages of breast cancer (BC). We identified 18 differentially expressed oxylipins between BC vs. HC patients, including anandamide, prostaglandins, and hydroxydocosahexaenoic acids. To integrate lipidomics, transcriptomics, and genomics data, we analyzed a transcriptome of 10 open database datasets obtained from tissues and blood cells of BC patients and SNP data for 33 genes related to oxylipin metabolism and revealed 19 changed genes, among them CYP2C19, PTGS2, HPGD, and FAAH included in the list of DEGs in the analysis of transcriptomes and the list of SNPs associated with BC. Our data allow us to suppose that oxylipin profiles can be used to evaluate the early stages of breast cancer. ABSTRACT: The involvement of oxylipins, metabolites of polyunsaturated fatty acids, in cancer pathogenesis was known long ago, but only the development of the high-throughput methods get the opportunity to study oxylipins on a system level. The study aimed to elucidate alterations in oxylipin metabolism as characteristics of breast cancer patients. We compared the ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) oxylipin profile signatures in the blood plasma of 152 healthy volunteers (HC) and 169 patients with different stages of breast cancer (BC). To integrate lipidomics, transcriptomics, and genomics data, we analyzed a transcriptome of 10 open database datasets obtained from tissues and blood cells of BC patients and SNP data for 33 genes related to oxylipin metabolism. We identified 18 oxylipins, metabolites of omega-3 or omega-6 polyunsaturated fatty acids, that were differentially expressed between BCvsHC patients, including anandamide, prostaglandins and hydroxydocosahexaenoic acids. DEGs analysis of tissue and blood samples from BC patients revealed that 19 genes for oxylipin biosynthesis change their expression level, with CYP2C19, PTGS2, HPGD, and FAAH included in the list of DEGs in the analysis of transcriptomes and the list of SNPs associated with BC. Results allow us to suppose that oxylipin signatures reflect the organism’s level of response to the disease. Our data regarding changes in oxylipins at the system level show that oxylipin profiles can be used to evaluate the early stages of breast cancer. MDPI 2022-04-18 /pmc/articles/PMC9032865/ /pubmed/35454947 http://dx.doi.org/10.3390/cancers14082041 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chistyakov, Dmitry V.
Guryleva, Mariia V.
Stepanova, Elena S.
Makarenkova, Lyubov M.
Ptitsyna, Elena V.
Goriainov, Sergei V.
Nikolskaya, Arina I.
Astakhova, Alina A.
Klimenko, Anna S.
Bezborodova, Olga A.
Rasskazova, Elena A.
Potanina, Olga G.
Abramovich, Rimma A.
Nemtsova, Elena R.
Sergeeva, Marina G.
Multi-Omics Approach Points to the Importance of Oxylipins Metabolism in Early-Stage Breast Cancer
title Multi-Omics Approach Points to the Importance of Oxylipins Metabolism in Early-Stage Breast Cancer
title_full Multi-Omics Approach Points to the Importance of Oxylipins Metabolism in Early-Stage Breast Cancer
title_fullStr Multi-Omics Approach Points to the Importance of Oxylipins Metabolism in Early-Stage Breast Cancer
title_full_unstemmed Multi-Omics Approach Points to the Importance of Oxylipins Metabolism in Early-Stage Breast Cancer
title_short Multi-Omics Approach Points to the Importance of Oxylipins Metabolism in Early-Stage Breast Cancer
title_sort multi-omics approach points to the importance of oxylipins metabolism in early-stage breast cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032865/
https://www.ncbi.nlm.nih.gov/pubmed/35454947
http://dx.doi.org/10.3390/cancers14082041
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