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Conventional and Real-Time PCR Targeting bla(OXA) Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains
Multidrug-resistant Acinetobacter baumannii, particularly those producing carbapenemases, are spread worldwide. A reliable detection of carbapenemases is essential to choose the appropriate antimicrobial therapy and, consequently, prevent the dissemination of carbapenem-resistant strains. The aim of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032895/ https://www.ncbi.nlm.nih.gov/pubmed/35453207 http://dx.doi.org/10.3390/antibiotics11040455 |
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author | Depka, Dagmara Mikucka, Agnieszka Bogiel, Tomasz Rzepka, Mateusz Zawadka, Patryk Gospodarek-Komkowska, Eugenia |
author_facet | Depka, Dagmara Mikucka, Agnieszka Bogiel, Tomasz Rzepka, Mateusz Zawadka, Patryk Gospodarek-Komkowska, Eugenia |
author_sort | Depka, Dagmara |
collection | PubMed |
description | Multidrug-resistant Acinetobacter baumannii, particularly those producing carbapenemases, are spread worldwide. A reliable detection of carbapenemases is essential to choose the appropriate antimicrobial therapy and, consequently, prevent the dissemination of carbapenem-resistant strains. The aim of this study is to examine the molecular basis of the carbapenem resistance mechanism and estimation of conventional PCR and real-time PCR usefulness for the detection of oxacillinases when compared to phenotypic carbapenemases detection. The following methods were evaluated: the CarbAcineto NP test, Carbapenem Inactivation Method, CPO panels of semiautomated antimicrobial susceptibility testing method on the BD Phoenix™ M50 system, conventional Polymerase Chain Reaction and real-time PCR. The eazyplex(®) SuperBug complete A assay was used as the reference method. Among the tested strains, 39 (67.2%) carried the bla(OXA-40) gene, while the bla(OXA-23) gene was noted amongst 19 (32.8%) isolates. The diagnostic sensitivities of the studied assays were as follows: CarbAcineto NP—65.5%; CIM—100%; CPO—100%; conventional PCR—100%; real-time PCR—100%. |
format | Online Article Text |
id | pubmed-9032895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90328952022-04-23 Conventional and Real-Time PCR Targeting bla(OXA) Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains Depka, Dagmara Mikucka, Agnieszka Bogiel, Tomasz Rzepka, Mateusz Zawadka, Patryk Gospodarek-Komkowska, Eugenia Antibiotics (Basel) Article Multidrug-resistant Acinetobacter baumannii, particularly those producing carbapenemases, are spread worldwide. A reliable detection of carbapenemases is essential to choose the appropriate antimicrobial therapy and, consequently, prevent the dissemination of carbapenem-resistant strains. The aim of this study is to examine the molecular basis of the carbapenem resistance mechanism and estimation of conventional PCR and real-time PCR usefulness for the detection of oxacillinases when compared to phenotypic carbapenemases detection. The following methods were evaluated: the CarbAcineto NP test, Carbapenem Inactivation Method, CPO panels of semiautomated antimicrobial susceptibility testing method on the BD Phoenix™ M50 system, conventional Polymerase Chain Reaction and real-time PCR. The eazyplex(®) SuperBug complete A assay was used as the reference method. Among the tested strains, 39 (67.2%) carried the bla(OXA-40) gene, while the bla(OXA-23) gene was noted amongst 19 (32.8%) isolates. The diagnostic sensitivities of the studied assays were as follows: CarbAcineto NP—65.5%; CIM—100%; CPO—100%; conventional PCR—100%; real-time PCR—100%. MDPI 2022-03-28 /pmc/articles/PMC9032895/ /pubmed/35453207 http://dx.doi.org/10.3390/antibiotics11040455 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Depka, Dagmara Mikucka, Agnieszka Bogiel, Tomasz Rzepka, Mateusz Zawadka, Patryk Gospodarek-Komkowska, Eugenia Conventional and Real-Time PCR Targeting bla(OXA) Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains |
title | Conventional and Real-Time PCR Targeting bla(OXA) Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains |
title_full | Conventional and Real-Time PCR Targeting bla(OXA) Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains |
title_fullStr | Conventional and Real-Time PCR Targeting bla(OXA) Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains |
title_full_unstemmed | Conventional and Real-Time PCR Targeting bla(OXA) Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains |
title_short | Conventional and Real-Time PCR Targeting bla(OXA) Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains |
title_sort | conventional and real-time pcr targeting bla(oxa) genes as reliable methods for a rapid detection of carbapenem-resistant acinetobacter baumannii clinical strains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032895/ https://www.ncbi.nlm.nih.gov/pubmed/35453207 http://dx.doi.org/10.3390/antibiotics11040455 |
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