Cargando…
Engineering a living cardiac pump on a chip using high-precision fabrication
Biomimetic on-chip tissue models serve as a powerful tool for studying human physiology and developing therapeutics; however, their modeling power is hindered by our inability to develop highly ordered functional structures in small length scales. Here, we demonstrate how high-precision fabrication...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032966/ https://www.ncbi.nlm.nih.gov/pubmed/35452278 http://dx.doi.org/10.1126/sciadv.abm3791 |
_version_ | 1784692775168507904 |
---|---|
author | Michas, Christos Karakan, M. Çağatay Nautiyal, Pranjal Seidman, Jonathan G. Seidman, Christine E. Agarwal, Arvind Ekinci, Kamil Eyckmans, Jeroen White, Alice E. Chen, Christopher S. |
author_facet | Michas, Christos Karakan, M. Çağatay Nautiyal, Pranjal Seidman, Jonathan G. Seidman, Christine E. Agarwal, Arvind Ekinci, Kamil Eyckmans, Jeroen White, Alice E. Chen, Christopher S. |
author_sort | Michas, Christos |
collection | PubMed |
description | Biomimetic on-chip tissue models serve as a powerful tool for studying human physiology and developing therapeutics; however, their modeling power is hindered by our inability to develop highly ordered functional structures in small length scales. Here, we demonstrate how high-precision fabrication can enable scaled-down modeling of organ-level cardiac mechanical function. We use two-photon direct laser writing (TPDLW) to fabricate a nanoscale-resolution metamaterial scaffold with fine-tuned mechanical properties to support the formation and cyclic contraction of a miniaturized, induced pluripotent stem cell–derived ventricular chamber. Furthermore, we fabricate microfluidic valves with extreme sensitivity to rectify the flow generated by the ventricular chamber. The integrated microfluidic system recapitulates the ventricular fluidic function and exhibits a complete pressure-volume loop with isovolumetric phases. Together, our results demonstrate a previously unexplored application of high-precision fabrication that can be generalized to expand the accessible spectrum of organ-on-a-chip models toward structurally and biomechanically sophisticated tissue systems. |
format | Online Article Text |
id | pubmed-9032966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-90329662022-05-04 Engineering a living cardiac pump on a chip using high-precision fabrication Michas, Christos Karakan, M. Çağatay Nautiyal, Pranjal Seidman, Jonathan G. Seidman, Christine E. Agarwal, Arvind Ekinci, Kamil Eyckmans, Jeroen White, Alice E. Chen, Christopher S. Sci Adv Physical and Materials Sciences Biomimetic on-chip tissue models serve as a powerful tool for studying human physiology and developing therapeutics; however, their modeling power is hindered by our inability to develop highly ordered functional structures in small length scales. Here, we demonstrate how high-precision fabrication can enable scaled-down modeling of organ-level cardiac mechanical function. We use two-photon direct laser writing (TPDLW) to fabricate a nanoscale-resolution metamaterial scaffold with fine-tuned mechanical properties to support the formation and cyclic contraction of a miniaturized, induced pluripotent stem cell–derived ventricular chamber. Furthermore, we fabricate microfluidic valves with extreme sensitivity to rectify the flow generated by the ventricular chamber. The integrated microfluidic system recapitulates the ventricular fluidic function and exhibits a complete pressure-volume loop with isovolumetric phases. Together, our results demonstrate a previously unexplored application of high-precision fabrication that can be generalized to expand the accessible spectrum of organ-on-a-chip models toward structurally and biomechanically sophisticated tissue systems. American Association for the Advancement of Science 2022-04-22 /pmc/articles/PMC9032966/ /pubmed/35452278 http://dx.doi.org/10.1126/sciadv.abm3791 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Physical and Materials Sciences Michas, Christos Karakan, M. Çağatay Nautiyal, Pranjal Seidman, Jonathan G. Seidman, Christine E. Agarwal, Arvind Ekinci, Kamil Eyckmans, Jeroen White, Alice E. Chen, Christopher S. Engineering a living cardiac pump on a chip using high-precision fabrication |
title | Engineering a living cardiac pump on a chip using high-precision fabrication |
title_full | Engineering a living cardiac pump on a chip using high-precision fabrication |
title_fullStr | Engineering a living cardiac pump on a chip using high-precision fabrication |
title_full_unstemmed | Engineering a living cardiac pump on a chip using high-precision fabrication |
title_short | Engineering a living cardiac pump on a chip using high-precision fabrication |
title_sort | engineering a living cardiac pump on a chip using high-precision fabrication |
topic | Physical and Materials Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032966/ https://www.ncbi.nlm.nih.gov/pubmed/35452278 http://dx.doi.org/10.1126/sciadv.abm3791 |
work_keys_str_mv | AT michaschristos engineeringalivingcardiacpumponachipusinghighprecisionfabrication AT karakanmcagatay engineeringalivingcardiacpumponachipusinghighprecisionfabrication AT nautiyalpranjal engineeringalivingcardiacpumponachipusinghighprecisionfabrication AT seidmanjonathang engineeringalivingcardiacpumponachipusinghighprecisionfabrication AT seidmanchristinee engineeringalivingcardiacpumponachipusinghighprecisionfabrication AT agarwalarvind engineeringalivingcardiacpumponachipusinghighprecisionfabrication AT ekincikamil engineeringalivingcardiacpumponachipusinghighprecisionfabrication AT eyckmansjeroen engineeringalivingcardiacpumponachipusinghighprecisionfabrication AT whitealicee engineeringalivingcardiacpumponachipusinghighprecisionfabrication AT chenchristophers engineeringalivingcardiacpumponachipusinghighprecisionfabrication |