An Odorant Receptor from the Proboscis of the Cotton Bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) Narrowly Tuned to Indole

SIMPLE SUMMARY: Odorant receptors (ORs) are at the core of the high-efficiency and sensitive olfactory system in insects. The expression and specific function of ORs largely contribute to the habits and speciation of one species. Although being predominantly expressed in the antennae, ORs in non-olfactory organs are suggested to have particular roles in promoting the reproduction or host fitness of insects. Our previous work has identified four ORs in the mouthpart organs of Helicoverpa armigera. Here, we amplified the full-length sequences of HarmORs from the proboscis. Further functional characterization suggested that HarmOR30 narrowly tuned to indole, the vital nitrogen-containing compounds that mediate tritrophic interactions. Our study deepens the insight into the olfactory perception of H. armigera, and explored a candidate functional receptor target for studying the interaction between insects and their plant hosts. ABSTRACT: Helicoverpa armigera is a serious agricultural pest with polyphagous diets, widespread distribution, and causing severe damage. Among sixty-five candidate ORs in H. armigera, the co-receptor HarmOrco and three specific ORs with partial sequences were identified to be expressed in the proboscis by our previous work, whereas their exact function is not known yet. In this study, we first confirmed the expression of these ORs in the proboscis by full-length cloning, which obtained the complete coding region of HarmOrco, OR24, and OR30. We then performed functional identification of HarmOR24 and OR30 by co-expressing them respectively with HarmOrco in Xenopus oocytes eukaryotic expression system combined with two-electrode voltage-clamp physiology. By testing the response of HarmOR24/OR30-expressing oocytes against eighty structural-divergent compounds, respectively, HarmOR30 was characterized to narrowly tune to indole and showed a specific tuning spectrum compared to its ortholog in Spodoptera littoralis. As indole is a distinctive herbivore-induced plant volatile and floral scent component, HarmOR30 might play roles in foraging and mediating the interactions between H. armigera with its surrounding environment.