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Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children

Obesity develops early in childhood and is accompanied by early signs of adipose tissue (AT) dysfunction and metabolic disease in children. In order to analyse the molecular processes during obesity-related AT accumulation in children, we investigated genome-wide expression profiles in AT samples, i...

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Autores principales: Landgraf, Kathrin, Kühnapfel, Andreas, Schlanstein, Maria, Biemann, Ronald, Isermann, Berend, Kempf, Elena, Kirsten, Holger, Scholz, Markus, Körner, Antje
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033114/
https://www.ncbi.nlm.nih.gov/pubmed/35457174
http://dx.doi.org/10.3390/ijms23084349
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author Landgraf, Kathrin
Kühnapfel, Andreas
Schlanstein, Maria
Biemann, Ronald
Isermann, Berend
Kempf, Elena
Kirsten, Holger
Scholz, Markus
Körner, Antje
author_facet Landgraf, Kathrin
Kühnapfel, Andreas
Schlanstein, Maria
Biemann, Ronald
Isermann, Berend
Kempf, Elena
Kirsten, Holger
Scholz, Markus
Körner, Antje
author_sort Landgraf, Kathrin
collection PubMed
description Obesity develops early in childhood and is accompanied by early signs of adipose tissue (AT) dysfunction and metabolic disease in children. In order to analyse the molecular processes during obesity-related AT accumulation in children, we investigated genome-wide expression profiles in AT samples, isolated adipocytes, and stromal vascular fraction (SVF) cells and assessed their relation to obesity as well as biological and functional AT parameters. We detected alterations in gene expression associated with obesity and related parameters, i.e., BMI SDS, adipocyte size, macrophage infiltration, adiponectin, and/or leptin. While differential gene expression in AT and adipocytes shared an enrichment in metabolic pathways and pathways related to extracellular structural organisation, SVF cells showed an overrepresentation in inflammatory pathways. In adipocytes, we found the strongest positive association for epidermal growth factor-like protein 6 (EGFL6) with adipocyte hypertrophy. EGFL6 was also upregulated during in vitro adipocyte differentiation. In children, EGFL6 expression was positively correlated to parameters of AT dysfunction and metabolic disease such as macrophage infiltration into AT, hs-CRP, leptin levels, and HOMA-IR. In conclusion, we provide evidence for early alterations in AT gene expression related to AT dysfunction in children and identified EGFL6 as potentially being involved in processes underlying the pathogenesis of metabolic disease.
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spelling pubmed-90331142022-04-23 Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children Landgraf, Kathrin Kühnapfel, Andreas Schlanstein, Maria Biemann, Ronald Isermann, Berend Kempf, Elena Kirsten, Holger Scholz, Markus Körner, Antje Int J Mol Sci Article Obesity develops early in childhood and is accompanied by early signs of adipose tissue (AT) dysfunction and metabolic disease in children. In order to analyse the molecular processes during obesity-related AT accumulation in children, we investigated genome-wide expression profiles in AT samples, isolated adipocytes, and stromal vascular fraction (SVF) cells and assessed their relation to obesity as well as biological and functional AT parameters. We detected alterations in gene expression associated with obesity and related parameters, i.e., BMI SDS, adipocyte size, macrophage infiltration, adiponectin, and/or leptin. While differential gene expression in AT and adipocytes shared an enrichment in metabolic pathways and pathways related to extracellular structural organisation, SVF cells showed an overrepresentation in inflammatory pathways. In adipocytes, we found the strongest positive association for epidermal growth factor-like protein 6 (EGFL6) with adipocyte hypertrophy. EGFL6 was also upregulated during in vitro adipocyte differentiation. In children, EGFL6 expression was positively correlated to parameters of AT dysfunction and metabolic disease such as macrophage infiltration into AT, hs-CRP, leptin levels, and HOMA-IR. In conclusion, we provide evidence for early alterations in AT gene expression related to AT dysfunction in children and identified EGFL6 as potentially being involved in processes underlying the pathogenesis of metabolic disease. MDPI 2022-04-14 /pmc/articles/PMC9033114/ /pubmed/35457174 http://dx.doi.org/10.3390/ijms23084349 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Landgraf, Kathrin
Kühnapfel, Andreas
Schlanstein, Maria
Biemann, Ronald
Isermann, Berend
Kempf, Elena
Kirsten, Holger
Scholz, Markus
Körner, Antje
Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children
title Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children
title_full Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children
title_fullStr Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children
title_full_unstemmed Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children
title_short Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children
title_sort transcriptome analyses of adipose tissue samples identify egfl6 as a candidate gene involved in obesity-related adipose tissue dysfunction in children
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033114/
https://www.ncbi.nlm.nih.gov/pubmed/35457174
http://dx.doi.org/10.3390/ijms23084349
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