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Construction and Analysis of lncRNA-Associated ceRNA Network in Atherosclerotic Plaque Formation

Atherosclerosis (AS) is a vascular disease with plaque formation. Unstable plaques can be expected to result in cardiovascular disease, such as myocardial infarction and stroke. Studies have verified that long noncoding RNAs (lncRNAs) play a critical role in atherosclerotic plaque formation (APF), i...

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Autores principales: Li, Danyu, Ma, Yunci, Deng, Weiqian, Feng, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033352/
https://www.ncbi.nlm.nih.gov/pubmed/35463977
http://dx.doi.org/10.1155/2022/4895611
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author Li, Danyu
Ma, Yunci
Deng, Weiqian
Feng, Jianhua
author_facet Li, Danyu
Ma, Yunci
Deng, Weiqian
Feng, Jianhua
author_sort Li, Danyu
collection PubMed
description Atherosclerosis (AS) is a vascular disease with plaque formation. Unstable plaques can be expected to result in cardiovascular disease, such as myocardial infarction and stroke. Studies have verified that long noncoding RNAs (lncRNAs) play a critical role in atherosclerotic plaque formation (APF), including MALAT1, GAS5, and H19. A ceRNA network is a combination of these two interacting processes, which regulate the occurrence and progression of many diseases. However, lncRNA-associated ceRNA network in terms of APF is limited. This study sought to discover novel potential biomarkers and ceRNA network for APF. We designed a triple network based on the lncRNA-miRNA and mRNA-miRNA pairs obtained from lncRNASNP and starBase. Differentially expressed genes (DEGs) and lncRNAs in human vascular tissues derived from the Gene Expression Omnibus database (GSE43292, GSE97210) were systematically selected and analyzed. A ceRNA network was constructed by hypergeometric test, including 8 lncRNAs, 243 miRNAs, and 8 mRNAs. APF-related ceRNA structure was discovered for the first time by combining network analysis and statistical validation. Topological analysis determined the key lncRNAs with the highest centroid. GO and KEGG enrichment analysis indicated that the ceRNA network was primarily enriched in “regulation of platelet-derived growth factor receptor signaling pathway,” “negative regulation of leukocyte chemotaxis,” and “axonal fasciculation.” A functional lncRNA, HAND2-AS1, was identified in the ceRNA network, and the main miRNA (miRNA-570-3p) regulated by HAND2-AS1 was further screened. This present study elucidated the important function of lncRNA in the origination and progression of APF and indicated the potential use of these hub nodes as diagnostic biomarkers and therapeutic targets.
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spelling pubmed-90333522022-04-23 Construction and Analysis of lncRNA-Associated ceRNA Network in Atherosclerotic Plaque Formation Li, Danyu Ma, Yunci Deng, Weiqian Feng, Jianhua Biomed Res Int Research Article Atherosclerosis (AS) is a vascular disease with plaque formation. Unstable plaques can be expected to result in cardiovascular disease, such as myocardial infarction and stroke. Studies have verified that long noncoding RNAs (lncRNAs) play a critical role in atherosclerotic plaque formation (APF), including MALAT1, GAS5, and H19. A ceRNA network is a combination of these two interacting processes, which regulate the occurrence and progression of many diseases. However, lncRNA-associated ceRNA network in terms of APF is limited. This study sought to discover novel potential biomarkers and ceRNA network for APF. We designed a triple network based on the lncRNA-miRNA and mRNA-miRNA pairs obtained from lncRNASNP and starBase. Differentially expressed genes (DEGs) and lncRNAs in human vascular tissues derived from the Gene Expression Omnibus database (GSE43292, GSE97210) were systematically selected and analyzed. A ceRNA network was constructed by hypergeometric test, including 8 lncRNAs, 243 miRNAs, and 8 mRNAs. APF-related ceRNA structure was discovered for the first time by combining network analysis and statistical validation. Topological analysis determined the key lncRNAs with the highest centroid. GO and KEGG enrichment analysis indicated that the ceRNA network was primarily enriched in “regulation of platelet-derived growth factor receptor signaling pathway,” “negative regulation of leukocyte chemotaxis,” and “axonal fasciculation.” A functional lncRNA, HAND2-AS1, was identified in the ceRNA network, and the main miRNA (miRNA-570-3p) regulated by HAND2-AS1 was further screened. This present study elucidated the important function of lncRNA in the origination and progression of APF and indicated the potential use of these hub nodes as diagnostic biomarkers and therapeutic targets. Hindawi 2022-04-15 /pmc/articles/PMC9033352/ /pubmed/35463977 http://dx.doi.org/10.1155/2022/4895611 Text en Copyright © 2022 Danyu Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Danyu
Ma, Yunci
Deng, Weiqian
Feng, Jianhua
Construction and Analysis of lncRNA-Associated ceRNA Network in Atherosclerotic Plaque Formation
title Construction and Analysis of lncRNA-Associated ceRNA Network in Atherosclerotic Plaque Formation
title_full Construction and Analysis of lncRNA-Associated ceRNA Network in Atherosclerotic Plaque Formation
title_fullStr Construction and Analysis of lncRNA-Associated ceRNA Network in Atherosclerotic Plaque Formation
title_full_unstemmed Construction and Analysis of lncRNA-Associated ceRNA Network in Atherosclerotic Plaque Formation
title_short Construction and Analysis of lncRNA-Associated ceRNA Network in Atherosclerotic Plaque Formation
title_sort construction and analysis of lncrna-associated cerna network in atherosclerotic plaque formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033352/
https://www.ncbi.nlm.nih.gov/pubmed/35463977
http://dx.doi.org/10.1155/2022/4895611
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