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Electrochemical immunosensor based on mussel inspired coating for simultaneous detection and elimination of Staphylococcus aureus in drinks

Staphylococcus aureus (S. aureus) is one of the most commonly isolated foodborne pathogens, and is considered as a major cause of foodborne illnesses worldwide. However, the development of smart and accurate analytical methods for the simultaneous detection and elimination of S. aureus in matrices o...

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Autores principales: Wu, Wenjin, Yang, Yuping, Wang, Lan, Xu, Tingting, Wang, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033411/
https://www.ncbi.nlm.nih.gov/pubmed/35480920
http://dx.doi.org/10.1039/d0ra10249k
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author Wu, Wenjin
Yang, Yuping
Wang, Lan
Xu, Tingting
Wang, Rui
author_facet Wu, Wenjin
Yang, Yuping
Wang, Lan
Xu, Tingting
Wang, Rui
author_sort Wu, Wenjin
collection PubMed
description Staphylococcus aureus (S. aureus) is one of the most commonly isolated foodborne pathogens, and is considered as a major cause of foodborne illnesses worldwide. However, the development of smart and accurate analytical methods for the simultaneous detection and elimination of S. aureus in matrices of food or drinks remains challenging. In the present work, a mussel-inspired material, ε-poly-l-lysine-3,4-dihydroxy benzaldehyde (EPD), was designed and fabricated based on its Schiff base structure. Owing to the robust ability of the material to adhere onto wet electrode surfaces and the pH-responsive properties of EPD, the prepared immunosensor exhibited an excellent detection limit and linear range with on-demand antibacterial activity. In real milk samples, the average values obtained from the immunosensor were approximate to the standard results obtained from the plate count method, and the relative standard deviation was 3.16–6.54%, suggesting the good accuracy of the developed method. Moreover, it exhibited good selectivity, reproducibility, and stability, thus demonstrating the potential significant applications of the electrochemical immunosensor in drinks safety monitoring.
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spelling pubmed-90334112022-04-26 Electrochemical immunosensor based on mussel inspired coating for simultaneous detection and elimination of Staphylococcus aureus in drinks Wu, Wenjin Yang, Yuping Wang, Lan Xu, Tingting Wang, Rui RSC Adv Chemistry Staphylococcus aureus (S. aureus) is one of the most commonly isolated foodborne pathogens, and is considered as a major cause of foodborne illnesses worldwide. However, the development of smart and accurate analytical methods for the simultaneous detection and elimination of S. aureus in matrices of food or drinks remains challenging. In the present work, a mussel-inspired material, ε-poly-l-lysine-3,4-dihydroxy benzaldehyde (EPD), was designed and fabricated based on its Schiff base structure. Owing to the robust ability of the material to adhere onto wet electrode surfaces and the pH-responsive properties of EPD, the prepared immunosensor exhibited an excellent detection limit and linear range with on-demand antibacterial activity. In real milk samples, the average values obtained from the immunosensor were approximate to the standard results obtained from the plate count method, and the relative standard deviation was 3.16–6.54%, suggesting the good accuracy of the developed method. Moreover, it exhibited good selectivity, reproducibility, and stability, thus demonstrating the potential significant applications of the electrochemical immunosensor in drinks safety monitoring. The Royal Society of Chemistry 2021-05-20 /pmc/articles/PMC9033411/ /pubmed/35480920 http://dx.doi.org/10.1039/d0ra10249k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Wu, Wenjin
Yang, Yuping
Wang, Lan
Xu, Tingting
Wang, Rui
Electrochemical immunosensor based on mussel inspired coating for simultaneous detection and elimination of Staphylococcus aureus in drinks
title Electrochemical immunosensor based on mussel inspired coating for simultaneous detection and elimination of Staphylococcus aureus in drinks
title_full Electrochemical immunosensor based on mussel inspired coating for simultaneous detection and elimination of Staphylococcus aureus in drinks
title_fullStr Electrochemical immunosensor based on mussel inspired coating for simultaneous detection and elimination of Staphylococcus aureus in drinks
title_full_unstemmed Electrochemical immunosensor based on mussel inspired coating for simultaneous detection and elimination of Staphylococcus aureus in drinks
title_short Electrochemical immunosensor based on mussel inspired coating for simultaneous detection and elimination of Staphylococcus aureus in drinks
title_sort electrochemical immunosensor based on mussel inspired coating for simultaneous detection and elimination of staphylococcus aureus in drinks
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033411/
https://www.ncbi.nlm.nih.gov/pubmed/35480920
http://dx.doi.org/10.1039/d0ra10249k
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