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Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots
An ultrasensitive biosensing platform for DNA and ochratoxin A (OTA) detection is constructed based on the luminescence quenching ability of fullerenol quantum dots (FOQDs) for the first time. As the surface of FOQDs is largely covered by hydroxyl groups, stable colloidal suspension of FOQDS in aque...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033561/ https://www.ncbi.nlm.nih.gov/pubmed/35479209 http://dx.doi.org/10.1039/d1ra01680f |
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author | Li, Hui Pang, Hua Zhang, Liangxiao Mao, Jin Zhang, Wen Jiang, Jun Li, Peiwu Zhang, Qi |
author_facet | Li, Hui Pang, Hua Zhang, Liangxiao Mao, Jin Zhang, Wen Jiang, Jun Li, Peiwu Zhang, Qi |
author_sort | Li, Hui |
collection | PubMed |
description | An ultrasensitive biosensing platform for DNA and ochratoxin A (OTA) detection is constructed based on the luminescence quenching ability of fullerenol quantum dots (FOQDs) for the first time. As the surface of FOQDs is largely covered by hydroxyl groups, stable colloidal suspension of FOQDS in aqueous solution can be obtained, which is very advantageous for application in biosensing compared to nano-C(60). FOQDs can effectively quench the fluorescence of dyes with different emission wavelengths that are tagged to bioprobes to an extent of more than 87% in aqueous buffer solution through a PET mechanism. Moreover, the nonspecific quenching of the fluorescent dyes (not bound to bioprobes) caused by FOQDs is negligible, so the background signal is extremely low which is beneficial for improving the detection sensitivity. Based on the π–π stacking interaction between FOQDs and bioprobes, such as single-stranded (ss) DNA and aptamers, a nucleic acid assay with a detection of limit of 15 pM and a highly sensitive OTA assay with a detection limit of 5 pg mL(−1) in grape juice samples are developed through the simple “mix and measure” protocol based on luminescence quenching-and-recovery. This is the first demonstration of constructing biosensors utilizing the luminescence quenching ability of FOQDs through a PET mechanism, and the pronounced assay performance implies the promising potential of FOQDs in biosensing. |
format | Online Article Text |
id | pubmed-9033561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90335612022-04-26 Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots Li, Hui Pang, Hua Zhang, Liangxiao Mao, Jin Zhang, Wen Jiang, Jun Li, Peiwu Zhang, Qi RSC Adv Chemistry An ultrasensitive biosensing platform for DNA and ochratoxin A (OTA) detection is constructed based on the luminescence quenching ability of fullerenol quantum dots (FOQDs) for the first time. As the surface of FOQDs is largely covered by hydroxyl groups, stable colloidal suspension of FOQDS in aqueous solution can be obtained, which is very advantageous for application in biosensing compared to nano-C(60). FOQDs can effectively quench the fluorescence of dyes with different emission wavelengths that are tagged to bioprobes to an extent of more than 87% in aqueous buffer solution through a PET mechanism. Moreover, the nonspecific quenching of the fluorescent dyes (not bound to bioprobes) caused by FOQDs is negligible, so the background signal is extremely low which is beneficial for improving the detection sensitivity. Based on the π–π stacking interaction between FOQDs and bioprobes, such as single-stranded (ss) DNA and aptamers, a nucleic acid assay with a detection of limit of 15 pM and a highly sensitive OTA assay with a detection limit of 5 pg mL(−1) in grape juice samples are developed through the simple “mix and measure” protocol based on luminescence quenching-and-recovery. This is the first demonstration of constructing biosensors utilizing the luminescence quenching ability of FOQDs through a PET mechanism, and the pronounced assay performance implies the promising potential of FOQDs in biosensing. The Royal Society of Chemistry 2021-06-01 /pmc/articles/PMC9033561/ /pubmed/35479209 http://dx.doi.org/10.1039/d1ra01680f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Li, Hui Pang, Hua Zhang, Liangxiao Mao, Jin Zhang, Wen Jiang, Jun Li, Peiwu Zhang, Qi Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots |
title | Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots |
title_full | Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots |
title_fullStr | Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots |
title_full_unstemmed | Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots |
title_short | Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots |
title_sort | ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033561/ https://www.ncbi.nlm.nih.gov/pubmed/35479209 http://dx.doi.org/10.1039/d1ra01680f |
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