Cargando…
Mining the Vavilov wheat diversity panel for new sources of adult plant resistance to stripe rust
KEY MESSAGE: Multi-year evaluation of the Vavilov wheat diversity panel identified new sources of adult plant resistance to stripe rust. Genome-wide association studies revealed the key genomic regions influencing resistance, including seven novel loci. ABSTRACT: Wheat stripe rust (YR) caused by Puc...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033734/ https://www.ncbi.nlm.nih.gov/pubmed/35113190 http://dx.doi.org/10.1007/s00122-022-04037-8 |
_version_ | 1784692962389655552 |
---|---|
author | Jambuthenne, Dilani T. Riaz, Adnan Athiyannan, Naveenkumar Alahmad, Samir Ng, Wei Ling Ziems, Laura Afanasenko, Olga Periyannan, Sambasivam K. Aitken, Elizabeth Platz, Greg Godwin, Ian Voss-Fels, Kai P. Dinglasan, Eric Hickey, Lee T. |
author_facet | Jambuthenne, Dilani T. Riaz, Adnan Athiyannan, Naveenkumar Alahmad, Samir Ng, Wei Ling Ziems, Laura Afanasenko, Olga Periyannan, Sambasivam K. Aitken, Elizabeth Platz, Greg Godwin, Ian Voss-Fels, Kai P. Dinglasan, Eric Hickey, Lee T. |
author_sort | Jambuthenne, Dilani T. |
collection | PubMed |
description | KEY MESSAGE: Multi-year evaluation of the Vavilov wheat diversity panel identified new sources of adult plant resistance to stripe rust. Genome-wide association studies revealed the key genomic regions influencing resistance, including seven novel loci. ABSTRACT: Wheat stripe rust (YR) caused by Puccinia striiformis f. sp. tritici (Pst) poses a significant threat to global food security. Resistance genes commonly found in many wheat varieties have been rendered ineffective due to the rapid evolution of the pathogen. To identify novel sources of adult plant resistance (APR), 292 accessions from the N.I. Vavilov Institute of Plant Genetic Resources, Saint Petersburg, Russia, were screened for known APR genes (i.e. Yr18, Yr29, Yr46, Yr33, Yr39 and Yr59) using linked polymerase chain reaction (PCR) molecular markers. Accessions were evaluated against Pst (pathotype 134 E16 A + Yr17 + Yr27) at seedling and adult plant stages across multiple years (2014, 2015 and 2016) in Australia. Phenotypic analyses identified 132 lines that potentially carry novel sources of APR to YR. Genome-wide association studies (GWAS) identified 68 significant marker–trait associations (P < 0.001) for YR resistance, representing 47 independent quantitative trait loci (QTL) regions. Fourteen genomic regions overlapped with previously reported Yr genes, including Yr29, Yr56, Yr5, Yr43, Yr57, Yr30, Yr46, Yr47, Yr35, Yr36, Yrxy1, Yr59, Yr52 and YrYL. In total, seven QTL (positioned on chromosomes 1D, 2A, 3A, 3D, 5D, 7B and 7D) did not collocate with previously reported genes or QTL, indicating the presence of promising novel resistance factors. Overall, the Vavilov diversity panel provides a rich source of new alleles which could be used to broaden the genetic bases of YR resistance in modern wheat varieties. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00122-022-04037-8. |
format | Online Article Text |
id | pubmed-9033734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-90337342022-05-06 Mining the Vavilov wheat diversity panel for new sources of adult plant resistance to stripe rust Jambuthenne, Dilani T. Riaz, Adnan Athiyannan, Naveenkumar Alahmad, Samir Ng, Wei Ling Ziems, Laura Afanasenko, Olga Periyannan, Sambasivam K. Aitken, Elizabeth Platz, Greg Godwin, Ian Voss-Fels, Kai P. Dinglasan, Eric Hickey, Lee T. Theor Appl Genet Original Article KEY MESSAGE: Multi-year evaluation of the Vavilov wheat diversity panel identified new sources of adult plant resistance to stripe rust. Genome-wide association studies revealed the key genomic regions influencing resistance, including seven novel loci. ABSTRACT: Wheat stripe rust (YR) caused by Puccinia striiformis f. sp. tritici (Pst) poses a significant threat to global food security. Resistance genes commonly found in many wheat varieties have been rendered ineffective due to the rapid evolution of the pathogen. To identify novel sources of adult plant resistance (APR), 292 accessions from the N.I. Vavilov Institute of Plant Genetic Resources, Saint Petersburg, Russia, were screened for known APR genes (i.e. Yr18, Yr29, Yr46, Yr33, Yr39 and Yr59) using linked polymerase chain reaction (PCR) molecular markers. Accessions were evaluated against Pst (pathotype 134 E16 A + Yr17 + Yr27) at seedling and adult plant stages across multiple years (2014, 2015 and 2016) in Australia. Phenotypic analyses identified 132 lines that potentially carry novel sources of APR to YR. Genome-wide association studies (GWAS) identified 68 significant marker–trait associations (P < 0.001) for YR resistance, representing 47 independent quantitative trait loci (QTL) regions. Fourteen genomic regions overlapped with previously reported Yr genes, including Yr29, Yr56, Yr5, Yr43, Yr57, Yr30, Yr46, Yr47, Yr35, Yr36, Yrxy1, Yr59, Yr52 and YrYL. In total, seven QTL (positioned on chromosomes 1D, 2A, 3A, 3D, 5D, 7B and 7D) did not collocate with previously reported genes or QTL, indicating the presence of promising novel resistance factors. Overall, the Vavilov diversity panel provides a rich source of new alleles which could be used to broaden the genetic bases of YR resistance in modern wheat varieties. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00122-022-04037-8. Springer Berlin Heidelberg 2022-02-03 2022 /pmc/articles/PMC9033734/ /pubmed/35113190 http://dx.doi.org/10.1007/s00122-022-04037-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Jambuthenne, Dilani T. Riaz, Adnan Athiyannan, Naveenkumar Alahmad, Samir Ng, Wei Ling Ziems, Laura Afanasenko, Olga Periyannan, Sambasivam K. Aitken, Elizabeth Platz, Greg Godwin, Ian Voss-Fels, Kai P. Dinglasan, Eric Hickey, Lee T. Mining the Vavilov wheat diversity panel for new sources of adult plant resistance to stripe rust |
title | Mining the Vavilov wheat diversity panel for new sources of adult plant resistance to stripe rust |
title_full | Mining the Vavilov wheat diversity panel for new sources of adult plant resistance to stripe rust |
title_fullStr | Mining the Vavilov wheat diversity panel for new sources of adult plant resistance to stripe rust |
title_full_unstemmed | Mining the Vavilov wheat diversity panel for new sources of adult plant resistance to stripe rust |
title_short | Mining the Vavilov wheat diversity panel for new sources of adult plant resistance to stripe rust |
title_sort | mining the vavilov wheat diversity panel for new sources of adult plant resistance to stripe rust |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033734/ https://www.ncbi.nlm.nih.gov/pubmed/35113190 http://dx.doi.org/10.1007/s00122-022-04037-8 |
work_keys_str_mv | AT jambuthennedilanit miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT riazadnan miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT athiyannannaveenkumar miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT alahmadsamir miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT ngweiling miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT ziemslaura miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT afanasenkoolga miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT periyannansambasivamk miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT aitkenelizabeth miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT platzgreg miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT godwinian miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT vossfelskaip miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT dinglasaneric miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust AT hickeyleet miningthevavilovwheatdiversitypanelfornewsourcesofadultplantresistancetostriperust |