Cargando…
Evaluation of efficiency and sensitivity of 1D and 2D sample pooling strategies for SARS-CoV-2 RT-qPCR screening purposes
To increase the throughput, lower the cost, and save scarce test reagents, laboratories can pool patient samples before SARS-CoV-2 RT-qPCR testing. While different sample pooling methods have been proposed and effectively implemented in some laboratories, no systematic and large-scale evaluations ex...
Autores principales: | Verwilt, Jasper, Hellemans, Jan, Sante, Tom, Mestdagh, Pieter, Vandesompele, Jo |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033859/ https://www.ncbi.nlm.nih.gov/pubmed/35459775 http://dx.doi.org/10.1038/s41598-022-10581-6 |
Ejemplares similares
-
Accurate RT-qPCR gene expression analysis on cell culture lysates
por: Van Peer, Gert, et al.
Publicado: (2012) -
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR
por: Mestdagh, Pieter, et al.
Publicado: (2016) -
A novel and universal method for microRNA RT-qPCR data normalization
por: Mestdagh, Pieter, et al.
Publicado: (2009) -
Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples
por: Zeka, Fjoralba, et al.
Publicado: (2016) -
High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
por: Mestdagh, Pieter, et al.
Publicado: (2008)