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Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt
A “signal off” fluorescent aptasensor based on graphene oxide (GO) nanosheet and double-stranded DNA structure was fabricated for OTA detection. In the absence of OTA, the aptamer and its complementary DNA (cDNA) formed double-stranded conjugates that could coexist with GO, presenting fluorescence r...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034310/ https://www.ncbi.nlm.nih.gov/pubmed/35469312 http://dx.doi.org/10.1016/j.fochx.2022.100308 |
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author | Zhang, Qing Kang, Linzhi Yue, Pengfei Shi, Linchun Wang, Meng Zhou, Lidong Zhao, Haiping Kong, Weijun |
author_facet | Zhang, Qing Kang, Linzhi Yue, Pengfei Shi, Linchun Wang, Meng Zhou, Lidong Zhao, Haiping Kong, Weijun |
author_sort | Zhang, Qing |
collection | PubMed |
description | A “signal off” fluorescent aptasensor based on graphene oxide (GO) nanosheet and double-stranded DNA structure was fabricated for OTA detection. In the absence of OTA, the aptamer and its complementary DNA (cDNA) formed double-stranded conjugates that could coexist with GO, presenting fluorescence responses. Then, the presented OTA was captured by the aptamers, causing the release of cDNA-FAM probes. The free probes were adsorbed by GO, leading to an OTA concentration-dependent fluorescence quenching through fluorescence resonance energy transfer. Under optimum conditions, the fluorescent aptasensor exhibited outstanding sensitivity with a LOD of 11 pg/mL and a wide dynamic range of 0.04–30 ng/mL. The selectivity of the aptasensor was confirmed against other four mycotoxins, and the feasibility and reliability were verified by determining OTA in the spiked malt samples with satisfactory recovery of 95.50%-112.05%. This aptasensing platform could be adapted to measure other mycotoxins by replacing the aptamer sequences for food safety. |
format | Online Article Text |
id | pubmed-9034310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-90343102022-04-24 Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt Zhang, Qing Kang, Linzhi Yue, Pengfei Shi, Linchun Wang, Meng Zhou, Lidong Zhao, Haiping Kong, Weijun Food Chem X Analytical Methods A “signal off” fluorescent aptasensor based on graphene oxide (GO) nanosheet and double-stranded DNA structure was fabricated for OTA detection. In the absence of OTA, the aptamer and its complementary DNA (cDNA) formed double-stranded conjugates that could coexist with GO, presenting fluorescence responses. Then, the presented OTA was captured by the aptamers, causing the release of cDNA-FAM probes. The free probes were adsorbed by GO, leading to an OTA concentration-dependent fluorescence quenching through fluorescence resonance energy transfer. Under optimum conditions, the fluorescent aptasensor exhibited outstanding sensitivity with a LOD of 11 pg/mL and a wide dynamic range of 0.04–30 ng/mL. The selectivity of the aptasensor was confirmed against other four mycotoxins, and the feasibility and reliability were verified by determining OTA in the spiked malt samples with satisfactory recovery of 95.50%-112.05%. This aptasensing platform could be adapted to measure other mycotoxins by replacing the aptamer sequences for food safety. Elsevier 2022-04-11 /pmc/articles/PMC9034310/ /pubmed/35469312 http://dx.doi.org/10.1016/j.fochx.2022.100308 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Analytical Methods Zhang, Qing Kang, Linzhi Yue, Pengfei Shi, Linchun Wang, Meng Zhou, Lidong Zhao, Haiping Kong, Weijun Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt |
title | Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt |
title_full | Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt |
title_fullStr | Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt |
title_full_unstemmed | Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt |
title_short | Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt |
title_sort | development of a graphene oxide nanosheet and double-stranded dna structure based fluorescent “signal off” aptasensor for ochratoxin a detection in malt |
topic | Analytical Methods |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034310/ https://www.ncbi.nlm.nih.gov/pubmed/35469312 http://dx.doi.org/10.1016/j.fochx.2022.100308 |
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