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The miR-26 family regulates early B cell development and transformation
MiRNAs are small noncoding RNAs that promote the sequence-specific repression of their respective target genes, thereby regulating diverse physiological as well as pathological processes. Here, we identify a novel role of the miR-26 family in early B cell development. We show that enhanced expressio...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034462/ https://www.ncbi.nlm.nih.gov/pubmed/35459737 http://dx.doi.org/10.26508/lsa.202101303 |
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author | Hutter, Katharina Lindner, Silke E Kurschat, Constanze Rülicke, Thomas Villunger, Andreas Herzog, Sebastian |
author_facet | Hutter, Katharina Lindner, Silke E Kurschat, Constanze Rülicke, Thomas Villunger, Andreas Herzog, Sebastian |
author_sort | Hutter, Katharina |
collection | PubMed |
description | MiRNAs are small noncoding RNAs that promote the sequence-specific repression of their respective target genes, thereby regulating diverse physiological as well as pathological processes. Here, we identify a novel role of the miR-26 family in early B cell development. We show that enhanced expression of miR-26 family members potently blocks the pre-B to immature B cell transition, promotes pre-B cell expansion and eventually enables growth factor independency. Mechanistically, this is at least partially mediated by direct repression of the tumor-suppressor Pten, which consequently enhances PI3K-AKT signaling. Conversely, limiting miR-26 activity in a more physiological loss-of-function approach counteracts proliferation and enhances pre-B cell differentiation in vitro as well as in vivo. We therefore postulate a rheostat-like role for the miR-26 family in progenitor B cells, with an increase in mature miR-26 levels signaling cell expansion, and facilitating pre-B to the immature B cell progression when reduced. |
format | Online Article Text |
id | pubmed-9034462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-90344622022-05-06 The miR-26 family regulates early B cell development and transformation Hutter, Katharina Lindner, Silke E Kurschat, Constanze Rülicke, Thomas Villunger, Andreas Herzog, Sebastian Life Sci Alliance Research Articles MiRNAs are small noncoding RNAs that promote the sequence-specific repression of their respective target genes, thereby regulating diverse physiological as well as pathological processes. Here, we identify a novel role of the miR-26 family in early B cell development. We show that enhanced expression of miR-26 family members potently blocks the pre-B to immature B cell transition, promotes pre-B cell expansion and eventually enables growth factor independency. Mechanistically, this is at least partially mediated by direct repression of the tumor-suppressor Pten, which consequently enhances PI3K-AKT signaling. Conversely, limiting miR-26 activity in a more physiological loss-of-function approach counteracts proliferation and enhances pre-B cell differentiation in vitro as well as in vivo. We therefore postulate a rheostat-like role for the miR-26 family in progenitor B cells, with an increase in mature miR-26 levels signaling cell expansion, and facilitating pre-B to the immature B cell progression when reduced. Life Science Alliance LLC 2022-04-22 /pmc/articles/PMC9034462/ /pubmed/35459737 http://dx.doi.org/10.26508/lsa.202101303 Text en © 2022 Hutter et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Hutter, Katharina Lindner, Silke E Kurschat, Constanze Rülicke, Thomas Villunger, Andreas Herzog, Sebastian The miR-26 family regulates early B cell development and transformation |
title | The miR-26 family regulates early B cell development and transformation |
title_full | The miR-26 family regulates early B cell development and transformation |
title_fullStr | The miR-26 family regulates early B cell development and transformation |
title_full_unstemmed | The miR-26 family regulates early B cell development and transformation |
title_short | The miR-26 family regulates early B cell development and transformation |
title_sort | mir-26 family regulates early b cell development and transformation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034462/ https://www.ncbi.nlm.nih.gov/pubmed/35459737 http://dx.doi.org/10.26508/lsa.202101303 |
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