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Change of intestinal microbiota in mice model of bronchopulmonary dysplasia

BACKGROUND: Gut microbiota has been proposed to be related to the pathogenesis of pulmonary diseases such as asthma and lung cancer, according to the gut-lung axis. However, little is known about the roles of gut microbiota in the pathogenesis of bronchopulmonary dysplasia (BPD). This study was desi...

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Autores principales: Fan, Tianqun, Lu, Ling, Jin, Rong, Sui, Aihua, Guan, Renzheng, Cui, Fengjing, Qu, Zhenghai, Liu, Dongyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034698/
https://www.ncbi.nlm.nih.gov/pubmed/35469197
http://dx.doi.org/10.7717/peerj.13295
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author Fan, Tianqun
Lu, Ling
Jin, Rong
Sui, Aihua
Guan, Renzheng
Cui, Fengjing
Qu, Zhenghai
Liu, Dongyun
author_facet Fan, Tianqun
Lu, Ling
Jin, Rong
Sui, Aihua
Guan, Renzheng
Cui, Fengjing
Qu, Zhenghai
Liu, Dongyun
author_sort Fan, Tianqun
collection PubMed
description BACKGROUND: Gut microbiota has been proposed to be related to the pathogenesis of pulmonary diseases such as asthma and lung cancer, according to the gut-lung axis. However, little is known about the roles of gut microbiota in the pathogenesis of bronchopulmonary dysplasia (BPD). This study was designed to investigate the changes of gut microbiota in neonatal mice with BPD. METHODS: BPD model was induced through exposure to high concentration of oxygen. Hematoxylin and eosin (H&E) staining was utilized to determine the modeling efficiency. Stool samples were collected from the distal colon for the sequencing of V3–V4 regions of 16S rRNA, in order to analyze the gut microbiota diversity. RESULTS: Alpha diversity indicated that there were no statistical differences in the richness of gut microbiota between BPD model group and control group on day 7, 14 and 21. Beta diversity analysis showed that there were statistical differences in the gut microbiota on day 14 (R = 0.368, p = 0.021). Linear discriminant analysis effect size (LEfSe) showed that there were 22 markers with statistical differences on day 14 (p < 0.05), while those on day 7 and 21 were 3 and 4, respectively. Functional prediction analysis showed that the top three metabolic pathways were signal transduction (P(FDR) = 0.037), glycan biosynthesis and metabolism (P(FDR) = 0.032), and metabolism of terpenoids and polyketides (P(FDR) = 0.049). CONCLUSIONS: BPD mice showed disorder of gut microbiota, which may involve specific metabolic pathways in the early stage. With the progression of neonatal maturity, the differences of the gut microbiota between the two groups would gradually disappear.
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spelling pubmed-90346982022-04-24 Change of intestinal microbiota in mice model of bronchopulmonary dysplasia Fan, Tianqun Lu, Ling Jin, Rong Sui, Aihua Guan, Renzheng Cui, Fengjing Qu, Zhenghai Liu, Dongyun PeerJ Biochemistry BACKGROUND: Gut microbiota has been proposed to be related to the pathogenesis of pulmonary diseases such as asthma and lung cancer, according to the gut-lung axis. However, little is known about the roles of gut microbiota in the pathogenesis of bronchopulmonary dysplasia (BPD). This study was designed to investigate the changes of gut microbiota in neonatal mice with BPD. METHODS: BPD model was induced through exposure to high concentration of oxygen. Hematoxylin and eosin (H&E) staining was utilized to determine the modeling efficiency. Stool samples were collected from the distal colon for the sequencing of V3–V4 regions of 16S rRNA, in order to analyze the gut microbiota diversity. RESULTS: Alpha diversity indicated that there were no statistical differences in the richness of gut microbiota between BPD model group and control group on day 7, 14 and 21. Beta diversity analysis showed that there were statistical differences in the gut microbiota on day 14 (R = 0.368, p = 0.021). Linear discriminant analysis effect size (LEfSe) showed that there were 22 markers with statistical differences on day 14 (p < 0.05), while those on day 7 and 21 were 3 and 4, respectively. Functional prediction analysis showed that the top three metabolic pathways were signal transduction (P(FDR) = 0.037), glycan biosynthesis and metabolism (P(FDR) = 0.032), and metabolism of terpenoids and polyketides (P(FDR) = 0.049). CONCLUSIONS: BPD mice showed disorder of gut microbiota, which may involve specific metabolic pathways in the early stage. With the progression of neonatal maturity, the differences of the gut microbiota between the two groups would gradually disappear. PeerJ Inc. 2022-04-20 /pmc/articles/PMC9034698/ /pubmed/35469197 http://dx.doi.org/10.7717/peerj.13295 Text en © 2022 Fan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Fan, Tianqun
Lu, Ling
Jin, Rong
Sui, Aihua
Guan, Renzheng
Cui, Fengjing
Qu, Zhenghai
Liu, Dongyun
Change of intestinal microbiota in mice model of bronchopulmonary dysplasia
title Change of intestinal microbiota in mice model of bronchopulmonary dysplasia
title_full Change of intestinal microbiota in mice model of bronchopulmonary dysplasia
title_fullStr Change of intestinal microbiota in mice model of bronchopulmonary dysplasia
title_full_unstemmed Change of intestinal microbiota in mice model of bronchopulmonary dysplasia
title_short Change of intestinal microbiota in mice model of bronchopulmonary dysplasia
title_sort change of intestinal microbiota in mice model of bronchopulmonary dysplasia
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034698/
https://www.ncbi.nlm.nih.gov/pubmed/35469197
http://dx.doi.org/10.7717/peerj.13295
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