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A Novel Helper qPCR Assay for the Detection of miRNA Using Target/Helper Template for Primer Formation

A novel, simple, and sensitive quantitative polymerase chain reaction (qPCR) technology, which is termed as helper qPCR, was established to detect miRNA. In this assay, the target miRNA sequence was introduced as helper template for a reaction switch preforming two-step real-time qPCR strategy. Firs...

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Detalles Bibliográficos
Autores principales:	Jiang, Xianfeng, Wang, Jiahui, Shen, Xudan, Talap, Jadera, Yang, Yang, Yang, Dan, Xiao, Guizhou, Cai, Sheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034933/ https://www.ncbi.nlm.nih.gov/pubmed/35469146 http://dx.doi.org/10.1155/2022/6918054

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