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The Impact of Different Cell Culture Mediums on CD8+T Cells Expansion: A Bioinformatics Study

OBJECTIVE: Different Cell Culture medias can affect the expansion of T cells. The aim of this study is to assess signaling pathways, protein interactions and genes in T cells cultured in different common T cell expansion medias to select the best candidate. MATERIALS AND METHODS: In this in silico o...

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Autores principales: Jalili, Arsalan, Hajifathali, Abbas, Bereimipour, Ahmad, Roshandel, Elham, Aghdami, Nasser
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035229/
https://www.ncbi.nlm.nih.gov/pubmed/35451586
http://dx.doi.org/10.22074/cellj.2022.7779
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author Jalili, Arsalan
Hajifathali, Abbas
Bereimipour, Ahmad
Roshandel, Elham
Aghdami, Nasser
author_facet Jalili, Arsalan
Hajifathali, Abbas
Bereimipour, Ahmad
Roshandel, Elham
Aghdami, Nasser
author_sort Jalili, Arsalan
collection PubMed
description OBJECTIVE: Different Cell Culture medias can affect the expansion of T cells. The aim of this study is to assess signaling pathways, protein interactions and genes in T cells cultured in different common T cell expansion medias to select the best candidate. MATERIALS AND METHODS: In this in silico observational study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis. RESULTS: The results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in SREBP control of lipid synthesis, co-stimulatory signal during T-cell activation mitosis and chromosome dynamics, telomeres, telomerase, and cellular aging signal pathways. CONCLUSION: Using bioinformatics analyzes, integrated and regular genes were selected as common genes CD80, LST1, ATM and ITM2B 4-1BBL, Akt inhibitor, interleukin 7 and 15 expansion media.
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spelling pubmed-90352292022-05-05 The Impact of Different Cell Culture Mediums on CD8+T Cells Expansion: A Bioinformatics Study Jalili, Arsalan Hajifathali, Abbas Bereimipour, Ahmad Roshandel, Elham Aghdami, Nasser Cell J Original Article OBJECTIVE: Different Cell Culture medias can affect the expansion of T cells. The aim of this study is to assess signaling pathways, protein interactions and genes in T cells cultured in different common T cell expansion medias to select the best candidate. MATERIALS AND METHODS: In this in silico observational study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis. RESULTS: The results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in SREBP control of lipid synthesis, co-stimulatory signal during T-cell activation mitosis and chromosome dynamics, telomeres, telomerase, and cellular aging signal pathways. CONCLUSION: Using bioinformatics analyzes, integrated and regular genes were selected as common genes CD80, LST1, ATM and ITM2B 4-1BBL, Akt inhibitor, interleukin 7 and 15 expansion media. Royan Institute 2022-03 2022-03-13 /pmc/articles/PMC9035229/ /pubmed/35451586 http://dx.doi.org/10.22074/cellj.2022.7779 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jalili, Arsalan
Hajifathali, Abbas
Bereimipour, Ahmad
Roshandel, Elham
Aghdami, Nasser
The Impact of Different Cell Culture Mediums on CD8+T Cells Expansion: A Bioinformatics Study
title The Impact of Different Cell Culture Mediums on CD8+T Cells Expansion: A Bioinformatics Study
title_full The Impact of Different Cell Culture Mediums on CD8+T Cells Expansion: A Bioinformatics Study
title_fullStr The Impact of Different Cell Culture Mediums on CD8+T Cells Expansion: A Bioinformatics Study
title_full_unstemmed The Impact of Different Cell Culture Mediums on CD8+T Cells Expansion: A Bioinformatics Study
title_short The Impact of Different Cell Culture Mediums on CD8+T Cells Expansion: A Bioinformatics Study
title_sort impact of different cell culture mediums on cd8+t cells expansion: a bioinformatics study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035229/
https://www.ncbi.nlm.nih.gov/pubmed/35451586
http://dx.doi.org/10.22074/cellj.2022.7779
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