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Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study

BACKGROUND: A paucity of studies focused on the genetic association that tuberculosis (TB) patients with non-communicable diseases (NCDs) are more likely to be infected with Mycobacterium tuberculosis (MTB) with more potent virulence on anti-TB drug resistance than those without NCDs. The study aime...

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Autores principales: Guo, Shengqiong, Lei, Shiguang, Palittapongarnpim, Prasit, McNeil, Edward, Chaiprasert, Angkana, Li, Jinlan, Chen, Huijuan, Ou, Weizheng, Surachat, Komwit, Qin, Wan, Zhang, Siyu, Luo, Rujuan, Chongsuvivatwong, Virasakdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035274/
https://www.ncbi.nlm.nih.gov/pubmed/35462543
http://dx.doi.org/10.1186/s12879-022-07344-z
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author Guo, Shengqiong
Lei, Shiguang
Palittapongarnpim, Prasit
McNeil, Edward
Chaiprasert, Angkana
Li, Jinlan
Chen, Huijuan
Ou, Weizheng
Surachat, Komwit
Qin, Wan
Zhang, Siyu
Luo, Rujuan
Chongsuvivatwong, Virasakdi
author_facet Guo, Shengqiong
Lei, Shiguang
Palittapongarnpim, Prasit
McNeil, Edward
Chaiprasert, Angkana
Li, Jinlan
Chen, Huijuan
Ou, Weizheng
Surachat, Komwit
Qin, Wan
Zhang, Siyu
Luo, Rujuan
Chongsuvivatwong, Virasakdi
author_sort Guo, Shengqiong
collection PubMed
description BACKGROUND: A paucity of studies focused on the genetic association that tuberculosis (TB) patients with non-communicable diseases (NCDs) are more likely to be infected with Mycobacterium tuberculosis (MTB) with more potent virulence on anti-TB drug resistance than those without NCDs. The study aimed to document the predominant genotype, determine the association between MTB genotypes and NCD status and drug resistance. METHODS: We conducted a molecular study in 105 TB patients based on a cross-sectional study focused on the comorbid relationship between chronic conditions and TB among 1773 subjects from September 1, 2019 to August 30, 2020 in Guizhou, China. The participants were investigated through face-to-face interviews, followed by NCDs screening. The DNA of MTB isolates was extracted prior to genotyping using 24 loci MIRU-VNTR. The subsequent evaluations were performed by phylogenetic trees, combined with tests of statistical power, Chi-square or Fisher and multivariate logistic regression analysis. RESULTS: The Beijing family of Lineage 2 (East Asia) was the predominant genotype accounting for 43.8% (46/105), followed by Lineage 4 (Euro-America) strains, including Uganda I (34.3%, 36/105), and the NEW-1 (9.5%, 10/105). The proportion of Beijing strain in patients with and without NCDS was 28.6% (8/28) and 49.4% (38/77), respectively, with a statistical power test value of 24.3%. No significant association was detected between MTB genotype and NCD status. A low clustering rate (2.9%) was identified, consisting of two clusters. The rates of global, mono-, poly- and multi-drug resistance were 16.2% (17/105), 14.3% (15/105), 1.0% (1/105) and 4.8% (5/105), respectively. The drug-resistant rates of rifampicin, isoniazid, and streptomycin, were 6.7% (7/105), 11.4% (12/105) and 5.7% (6/105), respectively. Isoniazid resistance was significantly associated with the Beijing genotype of Lineage 2 (19.6% versus 5.1%). CONCLUSIONS: The Lineage 2 East Asia/Beijing genotype is the dominant genotype of the local MTB with endogenous infection preponderating. Not enough evidence is detected to support the association between the MTB genotype and diabetes/hypertension. Isoniazid resistance is associated with the Lineage 2 East Asia/Beijing strain. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-022-07344-z.
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spelling pubmed-90352742022-04-25 Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study Guo, Shengqiong Lei, Shiguang Palittapongarnpim, Prasit McNeil, Edward Chaiprasert, Angkana Li, Jinlan Chen, Huijuan Ou, Weizheng Surachat, Komwit Qin, Wan Zhang, Siyu Luo, Rujuan Chongsuvivatwong, Virasakdi BMC Infect Dis Research BACKGROUND: A paucity of studies focused on the genetic association that tuberculosis (TB) patients with non-communicable diseases (NCDs) are more likely to be infected with Mycobacterium tuberculosis (MTB) with more potent virulence on anti-TB drug resistance than those without NCDs. The study aimed to document the predominant genotype, determine the association between MTB genotypes and NCD status and drug resistance. METHODS: We conducted a molecular study in 105 TB patients based on a cross-sectional study focused on the comorbid relationship between chronic conditions and TB among 1773 subjects from September 1, 2019 to August 30, 2020 in Guizhou, China. The participants were investigated through face-to-face interviews, followed by NCDs screening. The DNA of MTB isolates was extracted prior to genotyping using 24 loci MIRU-VNTR. The subsequent evaluations were performed by phylogenetic trees, combined with tests of statistical power, Chi-square or Fisher and multivariate logistic regression analysis. RESULTS: The Beijing family of Lineage 2 (East Asia) was the predominant genotype accounting for 43.8% (46/105), followed by Lineage 4 (Euro-America) strains, including Uganda I (34.3%, 36/105), and the NEW-1 (9.5%, 10/105). The proportion of Beijing strain in patients with and without NCDS was 28.6% (8/28) and 49.4% (38/77), respectively, with a statistical power test value of 24.3%. No significant association was detected between MTB genotype and NCD status. A low clustering rate (2.9%) was identified, consisting of two clusters. The rates of global, mono-, poly- and multi-drug resistance were 16.2% (17/105), 14.3% (15/105), 1.0% (1/105) and 4.8% (5/105), respectively. The drug-resistant rates of rifampicin, isoniazid, and streptomycin, were 6.7% (7/105), 11.4% (12/105) and 5.7% (6/105), respectively. Isoniazid resistance was significantly associated with the Beijing genotype of Lineage 2 (19.6% versus 5.1%). CONCLUSIONS: The Lineage 2 East Asia/Beijing genotype is the dominant genotype of the local MTB with endogenous infection preponderating. Not enough evidence is detected to support the association between the MTB genotype and diabetes/hypertension. Isoniazid resistance is associated with the Lineage 2 East Asia/Beijing strain. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-022-07344-z. BioMed Central 2022-04-24 /pmc/articles/PMC9035274/ /pubmed/35462543 http://dx.doi.org/10.1186/s12879-022-07344-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Guo, Shengqiong
Lei, Shiguang
Palittapongarnpim, Prasit
McNeil, Edward
Chaiprasert, Angkana
Li, Jinlan
Chen, Huijuan
Ou, Weizheng
Surachat, Komwit
Qin, Wan
Zhang, Siyu
Luo, Rujuan
Chongsuvivatwong, Virasakdi
Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study
title Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study
title_full Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study
title_fullStr Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study
title_full_unstemmed Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study
title_short Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study
title_sort association between mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035274/
https://www.ncbi.nlm.nih.gov/pubmed/35462543
http://dx.doi.org/10.1186/s12879-022-07344-z
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