Cargando…

The expression patterns of MALAT-1, NEAT-1, THRIL, and miR-155-5p in the acute to the post-acute phase of COVID-19 disease

INTRODUCTION: One of the hallmarks of COVID-19 is overwhelming inflammation, which plays a very important role in the pathogenesis of COVID-19. Thus, identification of inflammatory factors that interact with the SARS-CoV-2 can be very important to control and diagnose the severity of COVID-19. The a...

Descripción completa

Detalles Bibliográficos
Autores principales: Abbasi-Kolli, Mohammad, Sadri Nahand, Javid, Kiani, Seyed Jalal, Khanaliha, Khadijeh, Khatami, AliReza, Taghizadieh, Mohammad, Torkamani, Ali Rajabi, Babakhaniyan, Kimiya, Bokharaei-Salim, Farah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035361/
https://www.ncbi.nlm.nih.gov/pubmed/35500644
http://dx.doi.org/10.1016/j.bjid.2022.102354
_version_ 1784693275697872896
author Abbasi-Kolli, Mohammad
Sadri Nahand, Javid
Kiani, Seyed Jalal
Khanaliha, Khadijeh
Khatami, AliReza
Taghizadieh, Mohammad
Torkamani, Ali Rajabi
Babakhaniyan, Kimiya
Bokharaei-Salim, Farah
author_facet Abbasi-Kolli, Mohammad
Sadri Nahand, Javid
Kiani, Seyed Jalal
Khanaliha, Khadijeh
Khatami, AliReza
Taghizadieh, Mohammad
Torkamani, Ali Rajabi
Babakhaniyan, Kimiya
Bokharaei-Salim, Farah
author_sort Abbasi-Kolli, Mohammad
collection PubMed
description INTRODUCTION: One of the hallmarks of COVID-19 is overwhelming inflammation, which plays a very important role in the pathogenesis of COVID-19. Thus, identification of inflammatory factors that interact with the SARS-CoV-2 can be very important to control and diagnose the severity of COVID-19. The aim of this study was to investigate the expression patterns of inflammation-related non-coding RNAs (ncRNAs) including MALAT-1, NEAT-1, THRIL, and miR-155-5p from the acute phase to the recovery phase of COVID-19. METHODS: Total RNA was extracted from Peripheral Blood Mononuclear Cell (PBMC) samples of 20 patients with acute COVID-19 infection and 20 healthy individuals and the expression levels of MALAT-1, NEAT-1, THRIL, and miR-155-5p were evaluated by real-time PCR assay. Besides, in order to monitor the expression pattern of selected ncRNAs from the acute phase to the recovery phase of COVID-19 disease, the levels of ncRNAs were re-measured 6‒7 weeks after the acute phase. RESULT: The mean expression levels of MALAT-1, THRIL, and miR-155-5p were significantly increased in the acute phase of COVID-19 compared with a healthy control group. In addition, the expression levels of MALAT-1 and THRIL in the post-acute phase of COVID-19 were significantly lower than in the acute phase of COVID-19. According to the ROC curve analysis, these ncRNAs could be considered useful biomarkers for COVID-19 diagnosis and for discriminating between acute and post-acute phase of COVID-19. DISCUSSION: Inflammation-related ncRNAs (MALAT-1, THRIL, and miR-150-5p) can act as hopeful biomarkers for the monitoring and diagnosis of COVID-19 disease.
format Online
Article
Text
id pubmed-9035361
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-90353612022-04-25 The expression patterns of MALAT-1, NEAT-1, THRIL, and miR-155-5p in the acute to the post-acute phase of COVID-19 disease Abbasi-Kolli, Mohammad Sadri Nahand, Javid Kiani, Seyed Jalal Khanaliha, Khadijeh Khatami, AliReza Taghizadieh, Mohammad Torkamani, Ali Rajabi Babakhaniyan, Kimiya Bokharaei-Salim, Farah Braz J Infect Dis Original Article INTRODUCTION: One of the hallmarks of COVID-19 is overwhelming inflammation, which plays a very important role in the pathogenesis of COVID-19. Thus, identification of inflammatory factors that interact with the SARS-CoV-2 can be very important to control and diagnose the severity of COVID-19. The aim of this study was to investigate the expression patterns of inflammation-related non-coding RNAs (ncRNAs) including MALAT-1, NEAT-1, THRIL, and miR-155-5p from the acute phase to the recovery phase of COVID-19. METHODS: Total RNA was extracted from Peripheral Blood Mononuclear Cell (PBMC) samples of 20 patients with acute COVID-19 infection and 20 healthy individuals and the expression levels of MALAT-1, NEAT-1, THRIL, and miR-155-5p were evaluated by real-time PCR assay. Besides, in order to monitor the expression pattern of selected ncRNAs from the acute phase to the recovery phase of COVID-19 disease, the levels of ncRNAs were re-measured 6‒7 weeks after the acute phase. RESULT: The mean expression levels of MALAT-1, THRIL, and miR-155-5p were significantly increased in the acute phase of COVID-19 compared with a healthy control group. In addition, the expression levels of MALAT-1 and THRIL in the post-acute phase of COVID-19 were significantly lower than in the acute phase of COVID-19. According to the ROC curve analysis, these ncRNAs could be considered useful biomarkers for COVID-19 diagnosis and for discriminating between acute and post-acute phase of COVID-19. DISCUSSION: Inflammation-related ncRNAs (MALAT-1, THRIL, and miR-150-5p) can act as hopeful biomarkers for the monitoring and diagnosis of COVID-19 disease. Elsevier 2022-04-25 /pmc/articles/PMC9035361/ /pubmed/35500644 http://dx.doi.org/10.1016/j.bjid.2022.102354 Text en © 2022 Sociedade Brasileira de Infectologia. Published by Elsevier España, S.L.U. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Abbasi-Kolli, Mohammad
Sadri Nahand, Javid
Kiani, Seyed Jalal
Khanaliha, Khadijeh
Khatami, AliReza
Taghizadieh, Mohammad
Torkamani, Ali Rajabi
Babakhaniyan, Kimiya
Bokharaei-Salim, Farah
The expression patterns of MALAT-1, NEAT-1, THRIL, and miR-155-5p in the acute to the post-acute phase of COVID-19 disease
title The expression patterns of MALAT-1, NEAT-1, THRIL, and miR-155-5p in the acute to the post-acute phase of COVID-19 disease
title_full The expression patterns of MALAT-1, NEAT-1, THRIL, and miR-155-5p in the acute to the post-acute phase of COVID-19 disease
title_fullStr The expression patterns of MALAT-1, NEAT-1, THRIL, and miR-155-5p in the acute to the post-acute phase of COVID-19 disease
title_full_unstemmed The expression patterns of MALAT-1, NEAT-1, THRIL, and miR-155-5p in the acute to the post-acute phase of COVID-19 disease
title_short The expression patterns of MALAT-1, NEAT-1, THRIL, and miR-155-5p in the acute to the post-acute phase of COVID-19 disease
title_sort expression patterns of malat-1, neat-1, thril, and mir-155-5p in the acute to the post-acute phase of covid-19 disease
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035361/
https://www.ncbi.nlm.nih.gov/pubmed/35500644
http://dx.doi.org/10.1016/j.bjid.2022.102354
work_keys_str_mv AT abbasikollimohammad theexpressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT sadrinahandjavid theexpressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT kianiseyedjalal theexpressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT khanalihakhadijeh theexpressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT khatamialireza theexpressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT taghizadiehmohammad theexpressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT torkamanialirajabi theexpressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT babakhaniyankimiya theexpressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT bokharaeisalimfarah theexpressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT abbasikollimohammad expressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT sadrinahandjavid expressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT kianiseyedjalal expressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT khanalihakhadijeh expressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT khatamialireza expressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT taghizadiehmohammad expressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT torkamanialirajabi expressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT babakhaniyankimiya expressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease
AT bokharaeisalimfarah expressionpatternsofmalat1neat1thrilandmir1555pintheacutetothepostacutephaseofcovid19disease