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Genetic and geographical delineation of zoonotic vector-borne helminths of canids

Several zoonotic vector-borne helminths (VBHs) infesting canids cause serious veterinary and medical diseases worldwide. Increasing the knowledge about their genetic structures is pivotal to identify them and therefore to settle effective surveillance and control measures. To overcome the limitation...

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Autores principales: Laidoudi, Younes, Bedjaoui, Samia, Latrofa, Maria Stefania, Fanelli, Angela, Dantas-Torres, Filipe, Otranto, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035454/
https://www.ncbi.nlm.nih.gov/pubmed/35462560
http://dx.doi.org/10.1038/s41598-022-10553-w
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author Laidoudi, Younes
Bedjaoui, Samia
Latrofa, Maria Stefania
Fanelli, Angela
Dantas-Torres, Filipe
Otranto, Domenico
author_facet Laidoudi, Younes
Bedjaoui, Samia
Latrofa, Maria Stefania
Fanelli, Angela
Dantas-Torres, Filipe
Otranto, Domenico
author_sort Laidoudi, Younes
collection PubMed
description Several zoonotic vector-borne helminths (VBHs) infesting canids cause serious veterinary and medical diseases worldwide. Increasing the knowledge about their genetic structures is pivotal to identify them and therefore to settle effective surveillance and control measures. To overcome the limitation due to the heterogeneity of large DNA sequence-datasets used for their genetic characterization, available cytochrome c oxidase subunit 1 (cox1) (n = 546) and the 12S rRNA (n = 280) sequences were examined using combined bioinformatic approach (i.e., distance-clustering, maximum likelihood phylogeny and phylogenetic evolutionary placement). Out of the 826 DNA available sequences from GenBank, 94.7% were characterized at the haplotype level regardless sequence size, completeness and/or their position. A total of 89 different haplotypes were delineated either by cox1 (n = 35), 12S rRNA (n = 21) or by both genes (n = 33), for 14 VBHs (e.g., Acanthocheilonema reconditum, Brugia spp., Dirofilaria immitis, Dirofilaria repens, Onchocerca lupi and Thelazia spp.). Overall, the present approach could be useful for studying global genetic diversity and phylogeography of VBHs. However, as barcoding sequences were restricted to two mitochondrial loci (cox1 and 12S rRNA), the haplotype delineation proposed herein should be confirmed by the characterization of other nuclear loci also to overcome potential limitations caused by the heteroplasmy phenomenon within the mitogenome of VBHs.
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spelling pubmed-90354542022-04-27 Genetic and geographical delineation of zoonotic vector-borne helminths of canids Laidoudi, Younes Bedjaoui, Samia Latrofa, Maria Stefania Fanelli, Angela Dantas-Torres, Filipe Otranto, Domenico Sci Rep Article Several zoonotic vector-borne helminths (VBHs) infesting canids cause serious veterinary and medical diseases worldwide. Increasing the knowledge about their genetic structures is pivotal to identify them and therefore to settle effective surveillance and control measures. To overcome the limitation due to the heterogeneity of large DNA sequence-datasets used for their genetic characterization, available cytochrome c oxidase subunit 1 (cox1) (n = 546) and the 12S rRNA (n = 280) sequences were examined using combined bioinformatic approach (i.e., distance-clustering, maximum likelihood phylogeny and phylogenetic evolutionary placement). Out of the 826 DNA available sequences from GenBank, 94.7% were characterized at the haplotype level regardless sequence size, completeness and/or their position. A total of 89 different haplotypes were delineated either by cox1 (n = 35), 12S rRNA (n = 21) or by both genes (n = 33), for 14 VBHs (e.g., Acanthocheilonema reconditum, Brugia spp., Dirofilaria immitis, Dirofilaria repens, Onchocerca lupi and Thelazia spp.). Overall, the present approach could be useful for studying global genetic diversity and phylogeography of VBHs. However, as barcoding sequences were restricted to two mitochondrial loci (cox1 and 12S rRNA), the haplotype delineation proposed herein should be confirmed by the characterization of other nuclear loci also to overcome potential limitations caused by the heteroplasmy phenomenon within the mitogenome of VBHs. Nature Publishing Group UK 2022-04-24 /pmc/articles/PMC9035454/ /pubmed/35462560 http://dx.doi.org/10.1038/s41598-022-10553-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Laidoudi, Younes
Bedjaoui, Samia
Latrofa, Maria Stefania
Fanelli, Angela
Dantas-Torres, Filipe
Otranto, Domenico
Genetic and geographical delineation of zoonotic vector-borne helminths of canids
title Genetic and geographical delineation of zoonotic vector-borne helminths of canids
title_full Genetic and geographical delineation of zoonotic vector-borne helminths of canids
title_fullStr Genetic and geographical delineation of zoonotic vector-borne helminths of canids
title_full_unstemmed Genetic and geographical delineation of zoonotic vector-borne helminths of canids
title_short Genetic and geographical delineation of zoonotic vector-borne helminths of canids
title_sort genetic and geographical delineation of zoonotic vector-borne helminths of canids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035454/
https://www.ncbi.nlm.nih.gov/pubmed/35462560
http://dx.doi.org/10.1038/s41598-022-10553-w
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