Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)

OBJECTIVES: Preventing reverse transcription loop-mediated isothermal amplification (RT-LAMP) carryover contamination could be solved by adding deoxyuridine triphosphate (dUTP) and uracil-DNA glycosylase (UDG) into the reaction master mix. METHODS: RNA was extracted from nasopharyngeal swab samples...

Descripción completa

Detalles Bibliográficos
Autores principales: Lai, Meng Yee, Bukhari, Fatma Diyana Mohd, Zulkefli, Nur Zulaikha, Ismail, Ilyiana, Mustapa, Nur Izati, Soh, Tuan Suhaila Tuan, Hassan, Afifah Haji, Peariasamy, Kalaiarasu M, Lee, Yee Leng, Suppiah, Jeyanthi, Thayan, Ravindran, Lau, Yee Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035611/
https://www.ncbi.nlm.nih.gov/pubmed/35472524
http://dx.doi.org/10.1016/j.ijid.2022.04.036
_version_ 1784693332485603328
author Lai, Meng Yee
Bukhari, Fatma Diyana Mohd
Zulkefli, Nur Zulaikha
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M
Lee, Yee Leng
Suppiah, Jeyanthi
Thayan, Ravindran
Lau, Yee Ling
author_facet Lai, Meng Yee
Bukhari, Fatma Diyana Mohd
Zulkefli, Nur Zulaikha
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M
Lee, Yee Leng
Suppiah, Jeyanthi
Thayan, Ravindran
Lau, Yee Ling
author_sort Lai, Meng Yee
collection PubMed
description OBJECTIVES: Preventing reverse transcription loop-mediated isothermal amplification (RT-LAMP) carryover contamination could be solved by adding deoxyuridine triphosphate (dUTP) and uracil-DNA glycosylase (UDG) into the reaction master mix. METHODS: RNA was extracted from nasopharyngeal swab samples by a simple RNA extraction method. RESULTS: Testing of 77 samples demonstrated 91.2% sensitivity (95% confidence interval [CI]: 78–98.2%) and 100% specificity (95% confidence interval: 92–100%) using UDG RT-LAMP. CONCLUSION: This colorimetric UDG RT-LAMP is a simple-to-use, fast, and easy-to-interpret method, which could serve as an alternative for diagnosis of SARS-CoV-2 infection, especially in remote hospitals and laboratories with under-equipped medical facilities.
format Online
Article
Text
id pubmed-9035611
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
record_format MEDLINE/PubMed
spelling pubmed-90356112022-04-25 Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP) Lai, Meng Yee Bukhari, Fatma Diyana Mohd Zulkefli, Nur Zulaikha Ismail, Ilyiana Mustapa, Nur Izati Soh, Tuan Suhaila Tuan Hassan, Afifah Haji Peariasamy, Kalaiarasu M Lee, Yee Leng Suppiah, Jeyanthi Thayan, Ravindran Lau, Yee Ling Int J Infect Dis Short Communication OBJECTIVES: Preventing reverse transcription loop-mediated isothermal amplification (RT-LAMP) carryover contamination could be solved by adding deoxyuridine triphosphate (dUTP) and uracil-DNA glycosylase (UDG) into the reaction master mix. METHODS: RNA was extracted from nasopharyngeal swab samples by a simple RNA extraction method. RESULTS: Testing of 77 samples demonstrated 91.2% sensitivity (95% confidence interval [CI]: 78–98.2%) and 100% specificity (95% confidence interval: 92–100%) using UDG RT-LAMP. CONCLUSION: This colorimetric UDG RT-LAMP is a simple-to-use, fast, and easy-to-interpret method, which could serve as an alternative for diagnosis of SARS-CoV-2 infection, especially in remote hospitals and laboratories with under-equipped medical facilities. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2022-07 2022-04-25 /pmc/articles/PMC9035611/ /pubmed/35472524 http://dx.doi.org/10.1016/j.ijid.2022.04.036 Text en © 2022 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Lai, Meng Yee
Bukhari, Fatma Diyana Mohd
Zulkefli, Nur Zulaikha
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M
Lee, Yee Leng
Suppiah, Jeyanthi
Thayan, Ravindran
Lau, Yee Ling
Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title_full Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title_fullStr Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title_full_unstemmed Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title_short Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)
title_sort colorimetric detection of sars-cov-2 by uracil-dna glycosylase (udg) reverse transcription loop-mediated isothermal amplification (rt-lamp)
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035611/
https://www.ncbi.nlm.nih.gov/pubmed/35472524
http://dx.doi.org/10.1016/j.ijid.2022.04.036
work_keys_str_mv AT laimengyee colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT bukharifatmadiyanamohd colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT zulkeflinurzulaikha colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT ismaililyiana colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT mustapanurizati colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT sohtuansuhailatuan colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT hassanafifahhaji colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT peariasamykalaiarasum colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT leeyeeleng colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT suppiahjeyanthi colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT thayanravindran colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp
AT lauyeeling colorimetricdetectionofsarscov2byuracildnaglycosylaseudgreversetranscriptionloopmediatedisothermalamplificationrtlamp

Ejemplares similares