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Spoilage of tilapia by Pseudomonas putida with different adhesion abilities

Four Pseudomonas putida strains isolated from spoiled tilapia were divided into three adhesion abilities—high, medium, and low—by an in vitro mucus model. Four strains had no significant difference in spoilage ability to the inoculated fish fillets. However, according to the in vivo experiment, the...

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Detalles Bibliográficos
Autores principales: Zhang, Wen, Wei, Yunru, Jin, Xilin, Lv, Xucong, Liu, Zhibin, Ni, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035656/
https://www.ncbi.nlm.nih.gov/pubmed/35479657
http://dx.doi.org/10.1016/j.crfs.2022.04.002
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author Zhang, Wen
Wei, Yunru
Jin, Xilin
Lv, Xucong
Liu, Zhibin
Ni, Li
author_facet Zhang, Wen
Wei, Yunru
Jin, Xilin
Lv, Xucong
Liu, Zhibin
Ni, Li
author_sort Zhang, Wen
collection PubMed
description Four Pseudomonas putida strains isolated from spoiled tilapia were divided into three adhesion abilities—high, medium, and low—by an in vitro mucus model. Four strains had no significant difference in spoilage ability to the inoculated fish fillets. However, according to the in vivo experiment, the spoilage caused by the four P.putida was positively correlated with their adhesion abilities. High adhesion strains not only caused more TVB-N in chilled fish, but also activated the spoilage activity of intestinal flora. The diversity of intestinal flora and the changes in volatile components in fish were detected by high-throughput sequencing and SPME-GC/MS. The strains with high adhesion abilities significantly changed the intestinal flora, which led to a significant increase in low-grade aldehydes, indole, and esters in flesh of fish, as well as the production of a fishy and pungent odor. The intestinal adhesion ability of spoilage bacteria was considered the key factor in spoilage of chilled fish.
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spelling pubmed-90356562022-04-26 Spoilage of tilapia by Pseudomonas putida with different adhesion abilities Zhang, Wen Wei, Yunru Jin, Xilin Lv, Xucong Liu, Zhibin Ni, Li Curr Res Food Sci Research Article Four Pseudomonas putida strains isolated from spoiled tilapia were divided into three adhesion abilities—high, medium, and low—by an in vitro mucus model. Four strains had no significant difference in spoilage ability to the inoculated fish fillets. However, according to the in vivo experiment, the spoilage caused by the four P.putida was positively correlated with their adhesion abilities. High adhesion strains not only caused more TVB-N in chilled fish, but also activated the spoilage activity of intestinal flora. The diversity of intestinal flora and the changes in volatile components in fish were detected by high-throughput sequencing and SPME-GC/MS. The strains with high adhesion abilities significantly changed the intestinal flora, which led to a significant increase in low-grade aldehydes, indole, and esters in flesh of fish, as well as the production of a fishy and pungent odor. The intestinal adhesion ability of spoilage bacteria was considered the key factor in spoilage of chilled fish. Elsevier 2022-04-11 /pmc/articles/PMC9035656/ /pubmed/35479657 http://dx.doi.org/10.1016/j.crfs.2022.04.002 Text en © 2022 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Zhang, Wen
Wei, Yunru
Jin, Xilin
Lv, Xucong
Liu, Zhibin
Ni, Li
Spoilage of tilapia by Pseudomonas putida with different adhesion abilities
title Spoilage of tilapia by Pseudomonas putida with different adhesion abilities
title_full Spoilage of tilapia by Pseudomonas putida with different adhesion abilities
title_fullStr Spoilage of tilapia by Pseudomonas putida with different adhesion abilities
title_full_unstemmed Spoilage of tilapia by Pseudomonas putida with different adhesion abilities
title_short Spoilage of tilapia by Pseudomonas putida with different adhesion abilities
title_sort spoilage of tilapia by pseudomonas putida with different adhesion abilities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035656/
https://www.ncbi.nlm.nih.gov/pubmed/35479657
http://dx.doi.org/10.1016/j.crfs.2022.04.002
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