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Spoilage of tilapia by Pseudomonas putida with different adhesion abilities
Four Pseudomonas putida strains isolated from spoiled tilapia were divided into three adhesion abilities—high, medium, and low—by an in vitro mucus model. Four strains had no significant difference in spoilage ability to the inoculated fish fillets. However, according to the in vivo experiment, the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035656/ https://www.ncbi.nlm.nih.gov/pubmed/35479657 http://dx.doi.org/10.1016/j.crfs.2022.04.002 |
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author | Zhang, Wen Wei, Yunru Jin, Xilin Lv, Xucong Liu, Zhibin Ni, Li |
author_facet | Zhang, Wen Wei, Yunru Jin, Xilin Lv, Xucong Liu, Zhibin Ni, Li |
author_sort | Zhang, Wen |
collection | PubMed |
description | Four Pseudomonas putida strains isolated from spoiled tilapia were divided into three adhesion abilities—high, medium, and low—by an in vitro mucus model. Four strains had no significant difference in spoilage ability to the inoculated fish fillets. However, according to the in vivo experiment, the spoilage caused by the four P.putida was positively correlated with their adhesion abilities. High adhesion strains not only caused more TVB-N in chilled fish, but also activated the spoilage activity of intestinal flora. The diversity of intestinal flora and the changes in volatile components in fish were detected by high-throughput sequencing and SPME-GC/MS. The strains with high adhesion abilities significantly changed the intestinal flora, which led to a significant increase in low-grade aldehydes, indole, and esters in flesh of fish, as well as the production of a fishy and pungent odor. The intestinal adhesion ability of spoilage bacteria was considered the key factor in spoilage of chilled fish. |
format | Online Article Text |
id | pubmed-9035656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-90356562022-04-26 Spoilage of tilapia by Pseudomonas putida with different adhesion abilities Zhang, Wen Wei, Yunru Jin, Xilin Lv, Xucong Liu, Zhibin Ni, Li Curr Res Food Sci Research Article Four Pseudomonas putida strains isolated from spoiled tilapia were divided into three adhesion abilities—high, medium, and low—by an in vitro mucus model. Four strains had no significant difference in spoilage ability to the inoculated fish fillets. However, according to the in vivo experiment, the spoilage caused by the four P.putida was positively correlated with their adhesion abilities. High adhesion strains not only caused more TVB-N in chilled fish, but also activated the spoilage activity of intestinal flora. The diversity of intestinal flora and the changes in volatile components in fish were detected by high-throughput sequencing and SPME-GC/MS. The strains with high adhesion abilities significantly changed the intestinal flora, which led to a significant increase in low-grade aldehydes, indole, and esters in flesh of fish, as well as the production of a fishy and pungent odor. The intestinal adhesion ability of spoilage bacteria was considered the key factor in spoilage of chilled fish. Elsevier 2022-04-11 /pmc/articles/PMC9035656/ /pubmed/35479657 http://dx.doi.org/10.1016/j.crfs.2022.04.002 Text en © 2022 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Zhang, Wen Wei, Yunru Jin, Xilin Lv, Xucong Liu, Zhibin Ni, Li Spoilage of tilapia by Pseudomonas putida with different adhesion abilities |
title | Spoilage of tilapia by Pseudomonas putida with different adhesion abilities |
title_full | Spoilage of tilapia by Pseudomonas putida with different adhesion abilities |
title_fullStr | Spoilage of tilapia by Pseudomonas putida with different adhesion abilities |
title_full_unstemmed | Spoilage of tilapia by Pseudomonas putida with different adhesion abilities |
title_short | Spoilage of tilapia by Pseudomonas putida with different adhesion abilities |
title_sort | spoilage of tilapia by pseudomonas putida with different adhesion abilities |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035656/ https://www.ncbi.nlm.nih.gov/pubmed/35479657 http://dx.doi.org/10.1016/j.crfs.2022.04.002 |
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