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Genetic Basis of Dorper Sheep (Ovis aries) Revealed by Long-Read De Novo Genome Assembly
Dorper sheep (Ovis aries) (DPS), developed in the 1930s by crossing Dorset Horn and Blackhead Persian sheep in South Africa, is a world-famous composite breed for mutton production. The genetic basis underlying this breed is yet to be elucidated. Here, we report the sequencing and assembly of a high...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035736/ https://www.ncbi.nlm.nih.gov/pubmed/35480318 http://dx.doi.org/10.3389/fgene.2022.846449 |
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author | Qiao, Guoyan Xu, Pan Guo, Tingting Wu, Yi Lu, Xiaofang Zhang, Qingfeng He, Xue Zhu, Shaohua Zhao, Hongchang Lei, Zhihui Sun, Weibo Yang, Bohui Yue, Yaojing |
author_facet | Qiao, Guoyan Xu, Pan Guo, Tingting Wu, Yi Lu, Xiaofang Zhang, Qingfeng He, Xue Zhu, Shaohua Zhao, Hongchang Lei, Zhihui Sun, Weibo Yang, Bohui Yue, Yaojing |
author_sort | Qiao, Guoyan |
collection | PubMed |
description | Dorper sheep (Ovis aries) (DPS), developed in the 1930s by crossing Dorset Horn and Blackhead Persian sheep in South Africa, is a world-famous composite breed for mutton production. The genetic basis underlying this breed is yet to be elucidated. Here, we report the sequencing and assembly of a highly contiguous Dorper sheep genome via integration of Oxford Nanopore Technology (ONT) sequencing and Hi-C (chromatin conformation capture) approaches. The assembled genome was around 2.64 Gb with a contig N50 of 73.33 Mb and 140 contigs in total. More than 99.5% of the assembled sequences could be anchored to 27 chromosomes and they were annotated with 20,450 protein-coding genes. Allele-specific expression (ASE) genes of Dorper sheep were revealed through ASE analysis and they were involved in the immune system, lipid metabolism, and environmental adaptation. A total of 5,701 and 456 allelic sites were observed in the SNP and indels loci identified from relevant whole-genome resequencing data. These allelic SNP and INDEL sites were annotated in 1,002 and 294 genes, respectively. Moreover, we calculated the number of variant sites and related genes derived from the maternal and paternal ancestors, revealing the genetic basis of outstanding phenotypic performance of Dorper sheep. In conclusion, this study reports the first reference genome of Dorper sheep and reveals its genetic basis through ASE. This study also provides a pipeline for mining genetic information of composite breeds, which has an implication for future hybrid-breeding practices. |
format | Online Article Text |
id | pubmed-9035736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90357362022-04-26 Genetic Basis of Dorper Sheep (Ovis aries) Revealed by Long-Read De Novo Genome Assembly Qiao, Guoyan Xu, Pan Guo, Tingting Wu, Yi Lu, Xiaofang Zhang, Qingfeng He, Xue Zhu, Shaohua Zhao, Hongchang Lei, Zhihui Sun, Weibo Yang, Bohui Yue, Yaojing Front Genet Genetics Dorper sheep (Ovis aries) (DPS), developed in the 1930s by crossing Dorset Horn and Blackhead Persian sheep in South Africa, is a world-famous composite breed for mutton production. The genetic basis underlying this breed is yet to be elucidated. Here, we report the sequencing and assembly of a highly contiguous Dorper sheep genome via integration of Oxford Nanopore Technology (ONT) sequencing and Hi-C (chromatin conformation capture) approaches. The assembled genome was around 2.64 Gb with a contig N50 of 73.33 Mb and 140 contigs in total. More than 99.5% of the assembled sequences could be anchored to 27 chromosomes and they were annotated with 20,450 protein-coding genes. Allele-specific expression (ASE) genes of Dorper sheep were revealed through ASE analysis and they were involved in the immune system, lipid metabolism, and environmental adaptation. A total of 5,701 and 456 allelic sites were observed in the SNP and indels loci identified from relevant whole-genome resequencing data. These allelic SNP and INDEL sites were annotated in 1,002 and 294 genes, respectively. Moreover, we calculated the number of variant sites and related genes derived from the maternal and paternal ancestors, revealing the genetic basis of outstanding phenotypic performance of Dorper sheep. In conclusion, this study reports the first reference genome of Dorper sheep and reveals its genetic basis through ASE. This study also provides a pipeline for mining genetic information of composite breeds, which has an implication for future hybrid-breeding practices. Frontiers Media S.A. 2022-04-11 /pmc/articles/PMC9035736/ /pubmed/35480318 http://dx.doi.org/10.3389/fgene.2022.846449 Text en Copyright © 2022 Qiao, Xu, Guo, Wu, Lu, Zhang, He, Zhu, Zhao, Lei, Sun, Yang and Yue. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Qiao, Guoyan Xu, Pan Guo, Tingting Wu, Yi Lu, Xiaofang Zhang, Qingfeng He, Xue Zhu, Shaohua Zhao, Hongchang Lei, Zhihui Sun, Weibo Yang, Bohui Yue, Yaojing Genetic Basis of Dorper Sheep (Ovis aries) Revealed by Long-Read De Novo Genome Assembly |
title | Genetic Basis of Dorper Sheep (Ovis aries) Revealed by Long-Read De Novo Genome Assembly |
title_full | Genetic Basis of Dorper Sheep (Ovis aries) Revealed by Long-Read De Novo Genome Assembly |
title_fullStr | Genetic Basis of Dorper Sheep (Ovis aries) Revealed by Long-Read De Novo Genome Assembly |
title_full_unstemmed | Genetic Basis of Dorper Sheep (Ovis aries) Revealed by Long-Read De Novo Genome Assembly |
title_short | Genetic Basis of Dorper Sheep (Ovis aries) Revealed by Long-Read De Novo Genome Assembly |
title_sort | genetic basis of dorper sheep (ovis aries) revealed by long-read de novo genome assembly |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035736/ https://www.ncbi.nlm.nih.gov/pubmed/35480318 http://dx.doi.org/10.3389/fgene.2022.846449 |
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