Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here...

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Detalles Bibliográficos
Autores principales: Schomakers, Bauke V., Hermans, Jill, Jaspers, Yorrick R.J., Salomons, Gajja, Vaz, Frédéric M., van Weeghel, Michel, Houtkooper, Riekelt H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035783/
https://www.ncbi.nlm.nih.gov/pubmed/35479116
http://dx.doi.org/10.1016/j.xpro.2022.101302
Descripción
Sumario:We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).

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