Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here...

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Detalles Bibliográficos
Autores principales: Schomakers, Bauke V., Hermans, Jill, Jaspers, Yorrick R.J., Salomons, Gajja, Vaz, Frédéric M., van Weeghel, Michel, Houtkooper, Riekelt H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035783/
https://www.ncbi.nlm.nih.gov/pubmed/35479116
http://dx.doi.org/10.1016/j.xpro.2022.101302
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author Schomakers, Bauke V.
Hermans, Jill
Jaspers, Yorrick R.J.
Salomons, Gajja
Vaz, Frédéric M.
van Weeghel, Michel
Houtkooper, Riekelt H.
author_facet Schomakers, Bauke V.
Hermans, Jill
Jaspers, Yorrick R.J.
Salomons, Gajja
Vaz, Frédéric M.
van Weeghel, Michel
Houtkooper, Riekelt H.
author_sort Schomakers, Bauke V.
collection PubMed
description We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).
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spelling pubmed-90357832022-04-26 Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS Schomakers, Bauke V. Hermans, Jill Jaspers, Yorrick R.J. Salomons, Gajja Vaz, Frédéric M. van Weeghel, Michel Houtkooper, Riekelt H. STAR Protoc Protocol We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021). Elsevier 2022-04-16 /pmc/articles/PMC9035783/ /pubmed/35479116 http://dx.doi.org/10.1016/j.xpro.2022.101302 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Schomakers, Bauke V.
Hermans, Jill
Jaspers, Yorrick R.J.
Salomons, Gajja
Vaz, Frédéric M.
van Weeghel, Michel
Houtkooper, Riekelt H.
Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS
title Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS
title_full Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS
title_fullStr Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS
title_full_unstemmed Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS
title_short Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS
title_sort polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan lc-ms
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035783/
https://www.ncbi.nlm.nih.gov/pubmed/35479116
http://dx.doi.org/10.1016/j.xpro.2022.101302
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