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Detection of RNA-DNA hybrids by immunostaining in meiotic nuclei of Saccharomyces cerevisiae
RNA transcripts can anneal with template DNA strands to form RNA-DNA hybrids (or R-loops if the non-template DNA strands exist), which play a variety of roles in many physiological processes. Here, we provide an accessible and reproducible approach for immunofluorescent staining of RNA-DNA hybrids w...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035804/ https://www.ncbi.nlm.nih.gov/pubmed/35479113 http://dx.doi.org/10.1016/j.xpro.2022.101325 |