Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain

Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in...

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Autores principales: Brown, Jenny M., Phan, Bao Anh, Aalling, Nadia, Morton, Gregory J., Schwartz, Michael W., Scarlett, Jarrad M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035806/
https://www.ncbi.nlm.nih.gov/pubmed/35479117
http://dx.doi.org/10.1016/j.xpro.2022.101329
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author Brown, Jenny M.
Phan, Bao Anh
Aalling, Nadia
Morton, Gregory J.
Schwartz, Michael W.
Scarlett, Jarrad M.
author_facet Brown, Jenny M.
Phan, Bao Anh
Aalling, Nadia
Morton, Gregory J.
Schwartz, Michael W.
Scarlett, Jarrad M.
author_sort Brown, Jenny M.
collection PubMed
description Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).
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spelling pubmed-90358062022-04-26 Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain Brown, Jenny M. Phan, Bao Anh Aalling, Nadia Morton, Gregory J. Schwartz, Michael W. Scarlett, Jarrad M. STAR Protoc Protocol Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021). Elsevier 2022-04-15 /pmc/articles/PMC9035806/ /pubmed/35479117 http://dx.doi.org/10.1016/j.xpro.2022.101329 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Brown, Jenny M.
Phan, Bao Anh
Aalling, Nadia
Morton, Gregory J.
Schwartz, Michael W.
Scarlett, Jarrad M.
Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain
title Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain
title_full Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain
title_fullStr Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain
title_full_unstemmed Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain
title_short Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain
title_sort combined micro-osmotic pump infusion and intracerebroventricular injection to study fgf1 signaling pathways in the mouse brain
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035806/
https://www.ncbi.nlm.nih.gov/pubmed/35479117
http://dx.doi.org/10.1016/j.xpro.2022.101329
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