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Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty

Frailty is a critical aging-related syndrome but the underlying metabolic mechanism remains poorly understood. The aim of this study was to identify novel biomarkers and reveal potential mechanisms of frailty based on the integrated analysis of metabolome and gut microbiome. In this study, twenty su...

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Autores principales: Guo, Yan, Zhu, Guoqin, Wang, Fengliang, Zhang, Haoyu, Chen, Xin, Mao, Yan, Lv, Yifan, Xia, Fan, Jin, Yi, Ding, Guoxian, Yu, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035822/
https://www.ncbi.nlm.nih.gov/pubmed/35479954
http://dx.doi.org/10.3389/fmed.2022.827174
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author Guo, Yan
Zhu, Guoqin
Wang, Fengliang
Zhang, Haoyu
Chen, Xin
Mao, Yan
Lv, Yifan
Xia, Fan
Jin, Yi
Ding, Guoxian
Yu, Jing
author_facet Guo, Yan
Zhu, Guoqin
Wang, Fengliang
Zhang, Haoyu
Chen, Xin
Mao, Yan
Lv, Yifan
Xia, Fan
Jin, Yi
Ding, Guoxian
Yu, Jing
author_sort Guo, Yan
collection PubMed
description Frailty is a critical aging-related syndrome but the underlying metabolic mechanism remains poorly understood. The aim of this study was to identify novel biomarkers and reveal potential mechanisms of frailty based on the integrated analysis of metabolome and gut microbiome. In this study, twenty subjects consisted of five middle-aged adults and fifteen older adults, of which fifteen older subjects were divided into three groups: non-frail, pre-frail, and frail, with five subjects in each group. The presence of frailty, pre-frailty, or non-frailty was established according to the physical frailty phenotype (PFP). We applied non-targeted metabolomics to serum and feces samples and used 16S rDNA gene sequencing to detect the fecal microbiome. The associations between metabolites and gut microbiota were analyzed by the Spearman’s correlation analysis. Serum metabolic shifts in frailty mainly included fatty acids and derivatives, carbohydrates, and monosaccharides. Most of the metabolites belonging to these classes increased in the serum of frail older adults. Propylparaben was found to gradually decrease in non-frail, pre-frail, and frail older adults. Distinct changes in fecal metabolite profiles and gut microbiota were also found among middle-aged adults, non-frail and frail older subjects. The relative abundance of Faecalibacteriu, Roseburia, and Fusicatenibacter decreased while the abundance of Parabacteroides and Bacteroides increased in frailty. The above altered microbes were associated with the changed serum metabolites in frailty, which included dodecanedioic acid, D-ribose, D-(-)-mannitol, creatine and indole, and their related fecal metabolites. The changed microbiome and related metabolites may be used as the biomarkers of frailty and is worthy of further mechanistic studies.
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spelling pubmed-90358222022-04-26 Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty Guo, Yan Zhu, Guoqin Wang, Fengliang Zhang, Haoyu Chen, Xin Mao, Yan Lv, Yifan Xia, Fan Jin, Yi Ding, Guoxian Yu, Jing Front Med (Lausanne) Medicine Frailty is a critical aging-related syndrome but the underlying metabolic mechanism remains poorly understood. The aim of this study was to identify novel biomarkers and reveal potential mechanisms of frailty based on the integrated analysis of metabolome and gut microbiome. In this study, twenty subjects consisted of five middle-aged adults and fifteen older adults, of which fifteen older subjects were divided into three groups: non-frail, pre-frail, and frail, with five subjects in each group. The presence of frailty, pre-frailty, or non-frailty was established according to the physical frailty phenotype (PFP). We applied non-targeted metabolomics to serum and feces samples and used 16S rDNA gene sequencing to detect the fecal microbiome. The associations between metabolites and gut microbiota were analyzed by the Spearman’s correlation analysis. Serum metabolic shifts in frailty mainly included fatty acids and derivatives, carbohydrates, and monosaccharides. Most of the metabolites belonging to these classes increased in the serum of frail older adults. Propylparaben was found to gradually decrease in non-frail, pre-frail, and frail older adults. Distinct changes in fecal metabolite profiles and gut microbiota were also found among middle-aged adults, non-frail and frail older subjects. The relative abundance of Faecalibacteriu, Roseburia, and Fusicatenibacter decreased while the abundance of Parabacteroides and Bacteroides increased in frailty. The above altered microbes were associated with the changed serum metabolites in frailty, which included dodecanedioic acid, D-ribose, D-(-)-mannitol, creatine and indole, and their related fecal metabolites. The changed microbiome and related metabolites may be used as the biomarkers of frailty and is worthy of further mechanistic studies. Frontiers Media S.A. 2022-04-11 /pmc/articles/PMC9035822/ /pubmed/35479954 http://dx.doi.org/10.3389/fmed.2022.827174 Text en Copyright © 2022 Guo, Zhu, Wang, Zhang, Chen, Mao, Lv, Xia, Jin, Ding and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Guo, Yan
Zhu, Guoqin
Wang, Fengliang
Zhang, Haoyu
Chen, Xin
Mao, Yan
Lv, Yifan
Xia, Fan
Jin, Yi
Ding, Guoxian
Yu, Jing
Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty
title Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty
title_full Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty
title_fullStr Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty
title_full_unstemmed Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty
title_short Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty
title_sort distinct serum and fecal metabolite profiles linking with gut microbiome in older adults with frailty
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035822/
https://www.ncbi.nlm.nih.gov/pubmed/35479954
http://dx.doi.org/10.3389/fmed.2022.827174
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