Cargando…

Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes

Mupirocin has been reported for its role in the treatment of infected wounds through its antibacterial activity, however the role of mupirocin in promoting wound healing via alternative mechanisms has not been extensively evaluated. This study aimed to evaluate the potential effect of mupirocin to p...

Descripción completa

Detalles Bibliográficos
Autores principales: Twilley, Danielle, Reva, Oleg, Meyer, Debra, Lall, Namrita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035886/
https://www.ncbi.nlm.nih.gov/pubmed/35479318
http://dx.doi.org/10.3389/fphar.2022.862112
_version_ 1784693398723100672
author Twilley, Danielle
Reva, Oleg
Meyer, Debra
Lall, Namrita
author_facet Twilley, Danielle
Reva, Oleg
Meyer, Debra
Lall, Namrita
author_sort Twilley, Danielle
collection PubMed
description Mupirocin has been reported for its role in the treatment of infected wounds through its antibacterial activity, however the role of mupirocin in promoting wound healing via alternative mechanisms has not been extensively evaluated. This study aimed to evaluate the potential effect of mupirocin to promote wound healing, not only through its antibacterial activity but by increasing human keratinocyte proliferation and growth factor production. In the scratch assay, using human keratinocytes (HaCat), mupirocin (at 0.1 and 0.2 mM) significantly increased wound closure compared to the vehicle control. Cell viability, measured from the scratch assay, verified the increase in wound closure, where mupirocin at both concentrations showed higher cell viability compared to the vehicle control. In addition, mupirocin at 0.1 mM significantly stimulated the production of hepatocyte growth factor and M-CSF in HaCat cells, whereas at 0.2 mM, PDGF-AA and EPO were increased. The findings of this study suggest that mupirocin, which is commonly used as an antibacterial agent for the treatment of wounds, also facilitates the wound healing process by stimulating the proliferation of human keratinocytes and enhancing the production of several growth factors involved in wound healing. This is the first report on the effect of mupirocin on growth factors expressed by human keratinocytes as well as the stimulation of keratinocyte proliferation.
format Online
Article
Text
id pubmed-9035886
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90358862022-04-26 Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes Twilley, Danielle Reva, Oleg Meyer, Debra Lall, Namrita Front Pharmacol Pharmacology Mupirocin has been reported for its role in the treatment of infected wounds through its antibacterial activity, however the role of mupirocin in promoting wound healing via alternative mechanisms has not been extensively evaluated. This study aimed to evaluate the potential effect of mupirocin to promote wound healing, not only through its antibacterial activity but by increasing human keratinocyte proliferation and growth factor production. In the scratch assay, using human keratinocytes (HaCat), mupirocin (at 0.1 and 0.2 mM) significantly increased wound closure compared to the vehicle control. Cell viability, measured from the scratch assay, verified the increase in wound closure, where mupirocin at both concentrations showed higher cell viability compared to the vehicle control. In addition, mupirocin at 0.1 mM significantly stimulated the production of hepatocyte growth factor and M-CSF in HaCat cells, whereas at 0.2 mM, PDGF-AA and EPO were increased. The findings of this study suggest that mupirocin, which is commonly used as an antibacterial agent for the treatment of wounds, also facilitates the wound healing process by stimulating the proliferation of human keratinocytes and enhancing the production of several growth factors involved in wound healing. This is the first report on the effect of mupirocin on growth factors expressed by human keratinocytes as well as the stimulation of keratinocyte proliferation. Frontiers Media S.A. 2022-04-11 /pmc/articles/PMC9035886/ /pubmed/35479318 http://dx.doi.org/10.3389/fphar.2022.862112 Text en Copyright © 2022 Twilley, Reva, Meyer and Lall. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Twilley, Danielle
Reva, Oleg
Meyer, Debra
Lall, Namrita
Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes
title Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes
title_full Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes
title_fullStr Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes
title_full_unstemmed Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes
title_short Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes
title_sort mupirocin promotes wound healing by stimulating growth factor production and proliferation of human keratinocytes
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035886/
https://www.ncbi.nlm.nih.gov/pubmed/35479318
http://dx.doi.org/10.3389/fphar.2022.862112
work_keys_str_mv AT twilleydanielle mupirocinpromoteswoundhealingbystimulatinggrowthfactorproductionandproliferationofhumankeratinocytes
AT revaoleg mupirocinpromoteswoundhealingbystimulatinggrowthfactorproductionandproliferationofhumankeratinocytes
AT meyerdebra mupirocinpromoteswoundhealingbystimulatinggrowthfactorproductionandproliferationofhumankeratinocytes
AT lallnamrita mupirocinpromoteswoundhealingbystimulatinggrowthfactorproductionandproliferationofhumankeratinocytes