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TiO(2) Nanotubes Functionalized with Icariin for an Attenuated In Vitro Immune Response and Improved In Vivo Osseointegration

Due to their superior mechanical and chemical properties, titanium (Ti) and its alloys have been widely used as orthopedic implantable devices. However, their bioinertness represents a limitation, which can be overcome by employing various surface modifications, such as TiO(2) nanotube (TNT) fabrica...

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Autores principales: Negrescu, Andreea-Mariana, Mitran, Valentina, Draghicescu, Wanda, Popescu, Simona, Pirvu, Cristian, Ionascu, Iuliana, Soare, Teodoru, Uzun, Seralp, Croitoru, Sorin Mihai, Cimpean, Anisoara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036299/
https://www.ncbi.nlm.nih.gov/pubmed/35466225
http://dx.doi.org/10.3390/jfb13020043
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author Negrescu, Andreea-Mariana
Mitran, Valentina
Draghicescu, Wanda
Popescu, Simona
Pirvu, Cristian
Ionascu, Iuliana
Soare, Teodoru
Uzun, Seralp
Croitoru, Sorin Mihai
Cimpean, Anisoara
author_facet Negrescu, Andreea-Mariana
Mitran, Valentina
Draghicescu, Wanda
Popescu, Simona
Pirvu, Cristian
Ionascu, Iuliana
Soare, Teodoru
Uzun, Seralp
Croitoru, Sorin Mihai
Cimpean, Anisoara
author_sort Negrescu, Andreea-Mariana
collection PubMed
description Due to their superior mechanical and chemical properties, titanium (Ti) and its alloys have been widely used as orthopedic implantable devices. However, their bioinertness represents a limitation, which can be overcome by employing various surface modifications, such as TiO(2) nanotube (TNT) fabrication via electrochemical anodization. Anodic TNTs present tunable dimensions and unique structures, turning them into feasible drug delivery platforms. In the present work, TNTs were loaded with icariin (Ica) through an adhesive intermediate layer of polydopamine (DP), and their in vitro and in vivo biological performance was evaluated. The successful fabrication of the modified surfaces was verified by scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), and contact angle measurements (CA), while the in vitro release of Ica was evaluated via UV-VIS spectrophotometry. In terms of in vitro behaviour, comparative studies on RAW 264.7 macrophages demonstrated that the TNT substrates, especially TNT-DP-Ica, elicited a lower inflammatory response compared to the Ti support. Moreover, the in vivo implantation studies evinced generation of a reduced fibrotic capsule around this implant and increased thickness of the newly formed bone tissue at 1 month and 3 months post-implantation, respectively. Overall, our results indicate that the controlled release of Ica from TNT surfaces could result in an improved osseointegration process.
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spelling pubmed-90362992022-04-26 TiO(2) Nanotubes Functionalized with Icariin for an Attenuated In Vitro Immune Response and Improved In Vivo Osseointegration Negrescu, Andreea-Mariana Mitran, Valentina Draghicescu, Wanda Popescu, Simona Pirvu, Cristian Ionascu, Iuliana Soare, Teodoru Uzun, Seralp Croitoru, Sorin Mihai Cimpean, Anisoara J Funct Biomater Article Due to their superior mechanical and chemical properties, titanium (Ti) and its alloys have been widely used as orthopedic implantable devices. However, their bioinertness represents a limitation, which can be overcome by employing various surface modifications, such as TiO(2) nanotube (TNT) fabrication via electrochemical anodization. Anodic TNTs present tunable dimensions and unique structures, turning them into feasible drug delivery platforms. In the present work, TNTs were loaded with icariin (Ica) through an adhesive intermediate layer of polydopamine (DP), and their in vitro and in vivo biological performance was evaluated. The successful fabrication of the modified surfaces was verified by scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), and contact angle measurements (CA), while the in vitro release of Ica was evaluated via UV-VIS spectrophotometry. In terms of in vitro behaviour, comparative studies on RAW 264.7 macrophages demonstrated that the TNT substrates, especially TNT-DP-Ica, elicited a lower inflammatory response compared to the Ti support. Moreover, the in vivo implantation studies evinced generation of a reduced fibrotic capsule around this implant and increased thickness of the newly formed bone tissue at 1 month and 3 months post-implantation, respectively. Overall, our results indicate that the controlled release of Ica from TNT surfaces could result in an improved osseointegration process. MDPI 2022-04-14 /pmc/articles/PMC9036299/ /pubmed/35466225 http://dx.doi.org/10.3390/jfb13020043 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Negrescu, Andreea-Mariana
Mitran, Valentina
Draghicescu, Wanda
Popescu, Simona
Pirvu, Cristian
Ionascu, Iuliana
Soare, Teodoru
Uzun, Seralp
Croitoru, Sorin Mihai
Cimpean, Anisoara
TiO(2) Nanotubes Functionalized with Icariin for an Attenuated In Vitro Immune Response and Improved In Vivo Osseointegration
title TiO(2) Nanotubes Functionalized with Icariin for an Attenuated In Vitro Immune Response and Improved In Vivo Osseointegration
title_full TiO(2) Nanotubes Functionalized with Icariin for an Attenuated In Vitro Immune Response and Improved In Vivo Osseointegration
title_fullStr TiO(2) Nanotubes Functionalized with Icariin for an Attenuated In Vitro Immune Response and Improved In Vivo Osseointegration
title_full_unstemmed TiO(2) Nanotubes Functionalized with Icariin for an Attenuated In Vitro Immune Response and Improved In Vivo Osseointegration
title_short TiO(2) Nanotubes Functionalized with Icariin for an Attenuated In Vitro Immune Response and Improved In Vivo Osseointegration
title_sort tio(2) nanotubes functionalized with icariin for an attenuated in vitro immune response and improved in vivo osseointegration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036299/
https://www.ncbi.nlm.nih.gov/pubmed/35466225
http://dx.doi.org/10.3390/jfb13020043
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