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Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD: A Pilot Study

Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease commonly induced by cigarette smoke. The expression of miRNAs can be altered in patients with COPD and could be used as a biomarker. We aimed to identify a panel of miRNAs in bronchoalveolar lavage (BAL) to differentiate...

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Autores principales: Cerón-Pisa, Noemi, Iglesias, Amanda, Shafiek, Hanaa, Martín-Medina, Aina, Esteva-Socias, Margalida, Muncunill, Josep, Fleischer, Aarne, Verdú, Javier, Cosío, Borja G., Sauleda, Jaume
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036303/
https://www.ncbi.nlm.nih.gov/pubmed/35466228
http://dx.doi.org/10.3390/pathophysiology29020013
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author Cerón-Pisa, Noemi
Iglesias, Amanda
Shafiek, Hanaa
Martín-Medina, Aina
Esteva-Socias, Margalida
Muncunill, Josep
Fleischer, Aarne
Verdú, Javier
Cosío, Borja G.
Sauleda, Jaume
author_facet Cerón-Pisa, Noemi
Iglesias, Amanda
Shafiek, Hanaa
Martín-Medina, Aina
Esteva-Socias, Margalida
Muncunill, Josep
Fleischer, Aarne
Verdú, Javier
Cosío, Borja G.
Sauleda, Jaume
author_sort Cerón-Pisa, Noemi
collection PubMed
description Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease commonly induced by cigarette smoke. The expression of miRNAs can be altered in patients with COPD and could be used as a biomarker. We aimed to identify a panel of miRNAs in bronchoalveolar lavage (BAL) to differentiate COPD patients from smokers and non-smokers with normal lung function. Accordingly, forty-five subjects classified as COPD, smokers, and non-smokers (n = 15 per group) underwent clinical, functional characterization and bronchoscopy with BAL. The mean age of the studied population was 61.61 ± 12.95 years, BMI 25.72 ± 3.82 Kg/m(2), FEV1/FVC 68.37 ± 12.00%, and FEV1 80.07 ± 23.63% predicted. According to microarray analysis, three miRNAs of the most upregulated were chosen: miR-320c, miR-200c-3p, and miR-449c-5p. These miRNAs were validated by qPCR and were shown to be differently expressed in COPD patients. ROC analysis showed that these three miRNAs together had an area under the curve of 0.89 in differentiating COPD from controls. Moreover, in silico analysis of candidate miRNAs by DIANA-miRPath showed potential involvement in the EGFR and Hippo pathways. These results suggest a specific 3-miRNA signature that could be potentially used as a biomarker to distinguish COPD patients from smokers and non-smoker subjects.
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spelling pubmed-90363032022-04-26 Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD: A Pilot Study Cerón-Pisa, Noemi Iglesias, Amanda Shafiek, Hanaa Martín-Medina, Aina Esteva-Socias, Margalida Muncunill, Josep Fleischer, Aarne Verdú, Javier Cosío, Borja G. Sauleda, Jaume Pathophysiology Article Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease commonly induced by cigarette smoke. The expression of miRNAs can be altered in patients with COPD and could be used as a biomarker. We aimed to identify a panel of miRNAs in bronchoalveolar lavage (BAL) to differentiate COPD patients from smokers and non-smokers with normal lung function. Accordingly, forty-five subjects classified as COPD, smokers, and non-smokers (n = 15 per group) underwent clinical, functional characterization and bronchoscopy with BAL. The mean age of the studied population was 61.61 ± 12.95 years, BMI 25.72 ± 3.82 Kg/m(2), FEV1/FVC 68.37 ± 12.00%, and FEV1 80.07 ± 23.63% predicted. According to microarray analysis, three miRNAs of the most upregulated were chosen: miR-320c, miR-200c-3p, and miR-449c-5p. These miRNAs were validated by qPCR and were shown to be differently expressed in COPD patients. ROC analysis showed that these three miRNAs together had an area under the curve of 0.89 in differentiating COPD from controls. Moreover, in silico analysis of candidate miRNAs by DIANA-miRPath showed potential involvement in the EGFR and Hippo pathways. These results suggest a specific 3-miRNA signature that could be potentially used as a biomarker to distinguish COPD patients from smokers and non-smoker subjects. MDPI 2022-03-29 /pmc/articles/PMC9036303/ /pubmed/35466228 http://dx.doi.org/10.3390/pathophysiology29020013 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cerón-Pisa, Noemi
Iglesias, Amanda
Shafiek, Hanaa
Martín-Medina, Aina
Esteva-Socias, Margalida
Muncunill, Josep
Fleischer, Aarne
Verdú, Javier
Cosío, Borja G.
Sauleda, Jaume
Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD: A Pilot Study
title Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD: A Pilot Study
title_full Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD: A Pilot Study
title_fullStr Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD: A Pilot Study
title_full_unstemmed Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD: A Pilot Study
title_short Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD: A Pilot Study
title_sort hsa-mir-320c, hsa-mir-200c-3p, and hsa-mir-449c-5p as potential specific mirna biomarkers of copd: a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036303/
https://www.ncbi.nlm.nih.gov/pubmed/35466228
http://dx.doi.org/10.3390/pathophysiology29020013
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