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Formation and Evaluation of Complete Blood Count Proficiency Testing Program

Introduction: The haematology external quality assessment (EQA) scheme is the most commonly used service of quality assurance. The provision of complete blood count (CBC) materials must meet the quality requirements at a reasonable cost. These requirements are the most significant challenges for EQA...

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Autores principales: Vu, Huy Quang, Le, Oanh Hoang, Truong, Duan Cong, Nguyen, Dung Ngoc, Van, Triet Hy, Le, Van Thi Kieu, Vang, Linh Thi Truc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036311/
https://www.ncbi.nlm.nih.gov/pubmed/35466176
http://dx.doi.org/10.3390/hematolrep14020012
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author Vu, Huy Quang
Le, Oanh Hoang
Truong, Duan Cong
Nguyen, Dung Ngoc
Van, Triet Hy
Le, Van Thi Kieu
Vang, Linh Thi Truc
author_facet Vu, Huy Quang
Le, Oanh Hoang
Truong, Duan Cong
Nguyen, Dung Ngoc
Van, Triet Hy
Le, Van Thi Kieu
Vang, Linh Thi Truc
author_sort Vu, Huy Quang
collection PubMed
description Introduction: The haematology external quality assessment (EQA) scheme is the most commonly used service of quality assurance. The provision of complete blood count (CBC) materials must meet the quality requirements at a reasonable cost. These requirements are the most significant challenges for EQA organisers in Vietnam. This study’s objective was to evaluate the homogeneity, long-term stability, and peer-group performance of 10-parameter stabilised CBC EQA samples. Methods: The CBC EQA material was prepared using the following steps, including (1) adjusting levels of stabilised erythrocyte, leukocyte, and platelet samples, (2) mixing those cells into batches at three levels, and (3) dispensing and storing them at 2–6 °C. A set of 10 and 30 specimens were randomly chosen from each batch to study the homogeneity and long-term stability following ISO 13528:2015. In total, 166 samples at two levels were randomly distributed to 40 participants, which reported 83 automatic cell counters among six automated analyser models in the CBC EQA program. Results: The 10-parameter stabilised CBC EQA materials at three levels became homogeneous and stable in 12 weeks when preserved at 2–6 °C. Meanwhile, for five parameters (RBC, Hb, MCH, MCV, and MPV), this process was prolonged for up to 16 weeks in stock condition. In terms of peer-group performance, the CV (%) values increased at the low concentration for almost all parameters, especially in platelet counts. Conclusions: The stabilised CBC EQA samples prepared using the partial fixation method with aldehyde and gutaraldehyde in this study meet the ISO 13528:2015 requirements of homogeneity and long-term stability for the CBC EQA scheme. Analytical performance evaluation should categorise participant methods into peer groups.
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spelling pubmed-90363112022-04-26 Formation and Evaluation of Complete Blood Count Proficiency Testing Program Vu, Huy Quang Le, Oanh Hoang Truong, Duan Cong Nguyen, Dung Ngoc Van, Triet Hy Le, Van Thi Kieu Vang, Linh Thi Truc Hematol Rep Article Introduction: The haematology external quality assessment (EQA) scheme is the most commonly used service of quality assurance. The provision of complete blood count (CBC) materials must meet the quality requirements at a reasonable cost. These requirements are the most significant challenges for EQA organisers in Vietnam. This study’s objective was to evaluate the homogeneity, long-term stability, and peer-group performance of 10-parameter stabilised CBC EQA samples. Methods: The CBC EQA material was prepared using the following steps, including (1) adjusting levels of stabilised erythrocyte, leukocyte, and platelet samples, (2) mixing those cells into batches at three levels, and (3) dispensing and storing them at 2–6 °C. A set of 10 and 30 specimens were randomly chosen from each batch to study the homogeneity and long-term stability following ISO 13528:2015. In total, 166 samples at two levels were randomly distributed to 40 participants, which reported 83 automatic cell counters among six automated analyser models in the CBC EQA program. Results: The 10-parameter stabilised CBC EQA materials at three levels became homogeneous and stable in 12 weeks when preserved at 2–6 °C. Meanwhile, for five parameters (RBC, Hb, MCH, MCV, and MPV), this process was prolonged for up to 16 weeks in stock condition. In terms of peer-group performance, the CV (%) values increased at the low concentration for almost all parameters, especially in platelet counts. Conclusions: The stabilised CBC EQA samples prepared using the partial fixation method with aldehyde and gutaraldehyde in this study meet the ISO 13528:2015 requirements of homogeneity and long-term stability for the CBC EQA scheme. Analytical performance evaluation should categorise participant methods into peer groups. MDPI 2022-03-25 /pmc/articles/PMC9036311/ /pubmed/35466176 http://dx.doi.org/10.3390/hematolrep14020012 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vu, Huy Quang
Le, Oanh Hoang
Truong, Duan Cong
Nguyen, Dung Ngoc
Van, Triet Hy
Le, Van Thi Kieu
Vang, Linh Thi Truc
Formation and Evaluation of Complete Blood Count Proficiency Testing Program
title Formation and Evaluation of Complete Blood Count Proficiency Testing Program
title_full Formation and Evaluation of Complete Blood Count Proficiency Testing Program
title_fullStr Formation and Evaluation of Complete Blood Count Proficiency Testing Program
title_full_unstemmed Formation and Evaluation of Complete Blood Count Proficiency Testing Program
title_short Formation and Evaluation of Complete Blood Count Proficiency Testing Program
title_sort formation and evaluation of complete blood count proficiency testing program
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036311/
https://www.ncbi.nlm.nih.gov/pubmed/35466176
http://dx.doi.org/10.3390/hematolrep14020012
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