Measurement of mitochondrial respiratory chain enzymatic activities in Drosophila melanogaster samples

Mitochondrial respiratory chain (MRC) dysfunction is linked to mitochondrial disease as well as other common conditions such as diabetes, neurodegeneration, cancer, and aging. Thus, the evaluation of MRC enzymatic activities is fundamental for diagnostics and research purposes on experimental models...

Descripción completa

Detalles Bibliográficos
Autores principales: Brischigliaro, Michele, Frigo, Elena, Fernandez-Vizarra, Erika, Bernardi, Paolo, Viscomi, Carlo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036317/
https://www.ncbi.nlm.nih.gov/pubmed/35479112
http://dx.doi.org/10.1016/j.xpro.2022.101322
_version_ 1784693497657294848
author Brischigliaro, Michele
Frigo, Elena
Fernandez-Vizarra, Erika
Bernardi, Paolo
Viscomi, Carlo
author_facet Brischigliaro, Michele
Frigo, Elena
Fernandez-Vizarra, Erika
Bernardi, Paolo
Viscomi, Carlo
author_sort Brischigliaro, Michele
collection PubMed
description Mitochondrial respiratory chain (MRC) dysfunction is linked to mitochondrial disease as well as other common conditions such as diabetes, neurodegeneration, cancer, and aging. Thus, the evaluation of MRC enzymatic activities is fundamental for diagnostics and research purposes on experimental models. Here, we provide a verified and reliable protocol for mitochondria isolation from various D. melanogaster samples and subsequent measurement of the activity of MRC complexes I–V plus citrate synthase (CS) through UV-VIS spectrophotometry. For complete details on the use and execution of this protocol, please refer to Brischigliaro et al. (2021).
format Online
Article
Text
id pubmed-9036317
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-90363172022-04-26 Measurement of mitochondrial respiratory chain enzymatic activities in Drosophila melanogaster samples Brischigliaro, Michele Frigo, Elena Fernandez-Vizarra, Erika Bernardi, Paolo Viscomi, Carlo STAR Protoc Protocol Mitochondrial respiratory chain (MRC) dysfunction is linked to mitochondrial disease as well as other common conditions such as diabetes, neurodegeneration, cancer, and aging. Thus, the evaluation of MRC enzymatic activities is fundamental for diagnostics and research purposes on experimental models. Here, we provide a verified and reliable protocol for mitochondria isolation from various D. melanogaster samples and subsequent measurement of the activity of MRC complexes I–V plus citrate synthase (CS) through UV-VIS spectrophotometry. For complete details on the use and execution of this protocol, please refer to Brischigliaro et al. (2021). Elsevier 2022-04-15 /pmc/articles/PMC9036317/ /pubmed/35479112 http://dx.doi.org/10.1016/j.xpro.2022.101322 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Brischigliaro, Michele
Frigo, Elena
Fernandez-Vizarra, Erika
Bernardi, Paolo
Viscomi, Carlo
Measurement of mitochondrial respiratory chain enzymatic activities in Drosophila melanogaster samples
title Measurement of mitochondrial respiratory chain enzymatic activities in Drosophila melanogaster samples
title_full Measurement of mitochondrial respiratory chain enzymatic activities in Drosophila melanogaster samples
title_fullStr Measurement of mitochondrial respiratory chain enzymatic activities in Drosophila melanogaster samples
title_full_unstemmed Measurement of mitochondrial respiratory chain enzymatic activities in Drosophila melanogaster samples
title_short Measurement of mitochondrial respiratory chain enzymatic activities in Drosophila melanogaster samples
title_sort measurement of mitochondrial respiratory chain enzymatic activities in drosophila melanogaster samples
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036317/
https://www.ncbi.nlm.nih.gov/pubmed/35479112
http://dx.doi.org/10.1016/j.xpro.2022.101322
work_keys_str_mv AT brischigliaromichele measurementofmitochondrialrespiratorychainenzymaticactivitiesindrosophilamelanogastersamples
AT frigoelena measurementofmitochondrialrespiratorychainenzymaticactivitiesindrosophilamelanogastersamples
AT fernandezvizarraerika measurementofmitochondrialrespiratorychainenzymaticactivitiesindrosophilamelanogastersamples
AT bernardipaolo measurementofmitochondrialrespiratorychainenzymaticactivitiesindrosophilamelanogastersamples
AT viscomicarlo measurementofmitochondrialrespiratorychainenzymaticactivitiesindrosophilamelanogastersamples

Ejemplares similares