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Comparative Transcriptome Analysis of Onion in Response to Infection by Alternaria porri (Ellis) Cifferi

Purple blotch (PB) is one of the most destructive foliar diseases of onion and other alliums, caused by a necrotrophic fungal pathogen Alternaria porri. There are no reports on the molecular response of onion to PB infection. To elucidate the response of onion to A. porri infection, we consequently...

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Autores principales: Khandagale, Kiran, Roylawar, Praveen, Kulkarni, Onkar, Khambalkar, Pravin, Ade, Avinash, Kulkarni, Abhijeet, Singh, Major, Gawande, Suresh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036366/
https://www.ncbi.nlm.nih.gov/pubmed/35481153
http://dx.doi.org/10.3389/fpls.2022.857306
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author Khandagale, Kiran
Roylawar, Praveen
Kulkarni, Onkar
Khambalkar, Pravin
Ade, Avinash
Kulkarni, Abhijeet
Singh, Major
Gawande, Suresh
author_facet Khandagale, Kiran
Roylawar, Praveen
Kulkarni, Onkar
Khambalkar, Pravin
Ade, Avinash
Kulkarni, Abhijeet
Singh, Major
Gawande, Suresh
author_sort Khandagale, Kiran
collection PubMed
description Purple blotch (PB) is one of the most destructive foliar diseases of onion and other alliums, caused by a necrotrophic fungal pathogen Alternaria porri. There are no reports on the molecular response of onion to PB infection. To elucidate the response of onion to A. porri infection, we consequently carried out an RNAseq analysis of the resistant (Arka Kalyan; AK) and susceptible (Agrifound rose; AFR) genotype after an artificial infection. Through differential expression analyses between control and pathogen-treated plants, we identified 8,064 upregulated and 248 downregulated genes in AFR, while 832 upregulated and 564 downregulated genes were identified in AK. A further significant reprogramming in the gene expression profile was also demonstrated by a functional annotation analysis. Gene ontology (GO) terms, which are particularly involved in defense responses and signaling, are overrepresented in current analyses such as “oxidoreductase activity,” “chitin catabolic processes,” and “defense response.” Several key plant defense genes were differentially expressed on A. porri infection, which includes pathogenesis-related (PR) proteins, receptor-like kinases, phytohormone signaling, cell-wall integrity, cytochrome P450 monooxygenases, and transcription factors. Some of the genes were exclusively overexpressed in resistant genotype, namely, GABA transporter1, ankyrin repeat domain-containing protein, xyloglucan endotransglucosylase/hydrolase, and PR-5 (thaumatin-like). Antioxidant enzyme activities were observed to be increased after infection in both genotypes but higher activity was found in the resistant genotype, AK. This is the first report of transcriptome profiling in onion in response to PB infection and will serve as a resource for future studies to elucidate the molecular mechanism of onion-A. porri interaction and to improve PB resistance in onions.
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spelling pubmed-90363662022-04-26 Comparative Transcriptome Analysis of Onion in Response to Infection by Alternaria porri (Ellis) Cifferi Khandagale, Kiran Roylawar, Praveen Kulkarni, Onkar Khambalkar, Pravin Ade, Avinash Kulkarni, Abhijeet Singh, Major Gawande, Suresh Front Plant Sci Plant Science Purple blotch (PB) is one of the most destructive foliar diseases of onion and other alliums, caused by a necrotrophic fungal pathogen Alternaria porri. There are no reports on the molecular response of onion to PB infection. To elucidate the response of onion to A. porri infection, we consequently carried out an RNAseq analysis of the resistant (Arka Kalyan; AK) and susceptible (Agrifound rose; AFR) genotype after an artificial infection. Through differential expression analyses between control and pathogen-treated plants, we identified 8,064 upregulated and 248 downregulated genes in AFR, while 832 upregulated and 564 downregulated genes were identified in AK. A further significant reprogramming in the gene expression profile was also demonstrated by a functional annotation analysis. Gene ontology (GO) terms, which are particularly involved in defense responses and signaling, are overrepresented in current analyses such as “oxidoreductase activity,” “chitin catabolic processes,” and “defense response.” Several key plant defense genes were differentially expressed on A. porri infection, which includes pathogenesis-related (PR) proteins, receptor-like kinases, phytohormone signaling, cell-wall integrity, cytochrome P450 monooxygenases, and transcription factors. Some of the genes were exclusively overexpressed in resistant genotype, namely, GABA transporter1, ankyrin repeat domain-containing protein, xyloglucan endotransglucosylase/hydrolase, and PR-5 (thaumatin-like). Antioxidant enzyme activities were observed to be increased after infection in both genotypes but higher activity was found in the resistant genotype, AK. This is the first report of transcriptome profiling in onion in response to PB infection and will serve as a resource for future studies to elucidate the molecular mechanism of onion-A. porri interaction and to improve PB resistance in onions. Frontiers Media S.A. 2022-04-11 /pmc/articles/PMC9036366/ /pubmed/35481153 http://dx.doi.org/10.3389/fpls.2022.857306 Text en Copyright © 2022 Khandagale, Roylawar, Kulkarni, Khambalkar, Ade, Kulkarni, Singh and Gawande. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Khandagale, Kiran
Roylawar, Praveen
Kulkarni, Onkar
Khambalkar, Pravin
Ade, Avinash
Kulkarni, Abhijeet
Singh, Major
Gawande, Suresh
Comparative Transcriptome Analysis of Onion in Response to Infection by Alternaria porri (Ellis) Cifferi
title Comparative Transcriptome Analysis of Onion in Response to Infection by Alternaria porri (Ellis) Cifferi
title_full Comparative Transcriptome Analysis of Onion in Response to Infection by Alternaria porri (Ellis) Cifferi
title_fullStr Comparative Transcriptome Analysis of Onion in Response to Infection by Alternaria porri (Ellis) Cifferi
title_full_unstemmed Comparative Transcriptome Analysis of Onion in Response to Infection by Alternaria porri (Ellis) Cifferi
title_short Comparative Transcriptome Analysis of Onion in Response to Infection by Alternaria porri (Ellis) Cifferi
title_sort comparative transcriptome analysis of onion in response to infection by alternaria porri (ellis) cifferi
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036366/
https://www.ncbi.nlm.nih.gov/pubmed/35481153
http://dx.doi.org/10.3389/fpls.2022.857306
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