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Protocol for non-invasive assessment of spontaneous movements of group-housed animals using remote video monitoring

Frequent monitoring of laboratory animals is critical for ensuring animal welfare and experimental data collection. To minimize the adverse and confounding effects caused by current monitoring protocols and human presence, we developed a low-cost, non-invasive, remotely accessible, extensible infrar...

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Detalles Bibliográficos
Autores principales: Marcus, Alan David, Achanta, Satyanarayana, Jordt, Sven-Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036393/
https://www.ncbi.nlm.nih.gov/pubmed/35479115
http://dx.doi.org/10.1016/j.xpro.2022.101326
Descripción
Sumario:Frequent monitoring of laboratory animals is critical for ensuring animal welfare and experimental data collection. To minimize the adverse and confounding effects caused by current monitoring protocols and human presence, we developed a low-cost, non-invasive, remotely accessible, extensible infrared video monitoring system. This protocol describes the construction and operation of the system, followed by applying deep-learning neural networks to track group-housed, unmarked mice for objective behavioral quantification. This system can be adapted to a variety of home-cage environments and species.