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Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features

Naegleria gruberi is a unicellular eukaryote whose evolutionary distance from animals and fungi has made it useful for developing hypotheses about the last common eukaryotic ancestor. Naegleria amoebae lack a cytoplasmic microtubule cytoskeleton and assemble microtubules only during mitosis and thus...

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Autores principales: Velle, Katrina B., Kennard, Andrew S., Trupinić, Monika, Ivec, Arian, Swafford, Andrew J.M., Nolton, Emily, Rice, Luke M., Tolić, Iva M., Fritz-Laylin, Lillian K., Wadsworth, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036621/
https://www.ncbi.nlm.nih.gov/pubmed/35139359
http://dx.doi.org/10.1016/j.cub.2022.01.034
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author Velle, Katrina B.
Kennard, Andrew S.
Trupinić, Monika
Ivec, Arian
Swafford, Andrew J.M.
Nolton, Emily
Rice, Luke M.
Tolić, Iva M.
Fritz-Laylin, Lillian K.
Wadsworth, Patricia
author_facet Velle, Katrina B.
Kennard, Andrew S.
Trupinić, Monika
Ivec, Arian
Swafford, Andrew J.M.
Nolton, Emily
Rice, Luke M.
Tolić, Iva M.
Fritz-Laylin, Lillian K.
Wadsworth, Patricia
author_sort Velle, Katrina B.
collection PubMed
description Naegleria gruberi is a unicellular eukaryote whose evolutionary distance from animals and fungi has made it useful for developing hypotheses about the last common eukaryotic ancestor. Naegleria amoebae lack a cytoplasmic microtubule cytoskeleton and assemble microtubules only during mitosis and thus represent a unique system for studying the evolution and functional specificity of mitotic tubulins and the spindles they assemble. Previous studies show that Naegleria amoebae express a divergent α-tubulin during mitosis, and we now show that Naegleria amoebae express a second mitotic α- and two mitotic β-tubulins. The mitotic tubulins are evolutionarily divergent relative to typical α- and β-tubulins and contain residues that suggest distinct microtubule properties. These distinct residues are conserved in mitotic tubulin homologs of the “brain-eating amoeba” Naegleria fowleri, making them potential drug targets. Using quantitative light microscopy, we find that Naegleria’s mitotic spindle is a distinctive barrel-like structure built from a ring of microtubule bundles. Similar to those of other species, Naegleria’s spindle is twisted, and its length increases during mitosis, suggesting that these aspects of mitosis are ancestral features. Because bundle numbers change during metaphase, we hypothesize that the initial bundles represent kinetochore fibers and secondary bundles function as bridging fibers.
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spelling pubmed-90366212022-04-25 Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features Velle, Katrina B. Kennard, Andrew S. Trupinić, Monika Ivec, Arian Swafford, Andrew J.M. Nolton, Emily Rice, Luke M. Tolić, Iva M. Fritz-Laylin, Lillian K. Wadsworth, Patricia Curr Biol Article Naegleria gruberi is a unicellular eukaryote whose evolutionary distance from animals and fungi has made it useful for developing hypotheses about the last common eukaryotic ancestor. Naegleria amoebae lack a cytoplasmic microtubule cytoskeleton and assemble microtubules only during mitosis and thus represent a unique system for studying the evolution and functional specificity of mitotic tubulins and the spindles they assemble. Previous studies show that Naegleria amoebae express a divergent α-tubulin during mitosis, and we now show that Naegleria amoebae express a second mitotic α- and two mitotic β-tubulins. The mitotic tubulins are evolutionarily divergent relative to typical α- and β-tubulins and contain residues that suggest distinct microtubule properties. These distinct residues are conserved in mitotic tubulin homologs of the “brain-eating amoeba” Naegleria fowleri, making them potential drug targets. Using quantitative light microscopy, we find that Naegleria’s mitotic spindle is a distinctive barrel-like structure built from a ring of microtubule bundles. Similar to those of other species, Naegleria’s spindle is twisted, and its length increases during mitosis, suggesting that these aspects of mitosis are ancestral features. Because bundle numbers change during metaphase, we hypothesize that the initial bundles represent kinetochore fibers and secondary bundles function as bridging fibers. 2022-03-28 2022-02-08 /pmc/articles/PMC9036621/ /pubmed/35139359 http://dx.doi.org/10.1016/j.cub.2022.01.034 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Velle, Katrina B.
Kennard, Andrew S.
Trupinić, Monika
Ivec, Arian
Swafford, Andrew J.M.
Nolton, Emily
Rice, Luke M.
Tolić, Iva M.
Fritz-Laylin, Lillian K.
Wadsworth, Patricia
Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
title Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
title_full Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
title_fullStr Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
title_full_unstemmed Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
title_short Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
title_sort naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036621/
https://www.ncbi.nlm.nih.gov/pubmed/35139359
http://dx.doi.org/10.1016/j.cub.2022.01.034
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