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Fluorescent labeling and characterization of dicarboxylic cellulose nanocrystals prepared by sequential periodate–chlorite oxidation

High-performance fluorescent composites are key to the development and improvement of fluorescent molecular probe technology. In this study, cellulose nanocrystals (CNC) with high carboxyl concentrations were prepared via sequential periodate–chlorite oxidation. Then, fluorescent cellulose nanocryst...

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Autores principales: Sun, Xiaozheng, Xue, Yanhua, Li, Jianye, Yang, Yu, Bai, Yu, Chen, Yujia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036875/
https://www.ncbi.nlm.nih.gov/pubmed/35481032
http://dx.doi.org/10.1039/d1ra04812k
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author Sun, Xiaozheng
Xue, Yanhua
Li, Jianye
Yang, Yu
Bai, Yu
Chen, Yujia
author_facet Sun, Xiaozheng
Xue, Yanhua
Li, Jianye
Yang, Yu
Bai, Yu
Chen, Yujia
author_sort Sun, Xiaozheng
collection PubMed
description High-performance fluorescent composites are key to the development and improvement of fluorescent molecular probe technology. In this study, cellulose nanocrystals (CNC) with high carboxyl concentrations were prepared via sequential periodate–chlorite oxidation. Then, fluorescent cellulose nanocrystals (FCNC) were prepared by attaching 7-amino-4-methylcoumarin (AMC) onto CNC under 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) catalysis. The morphology and fluorescence properties of FCNC were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, elemental analysis, ultraviolet-visible absorbance, fluorescence spectrophotometry, and fluorescence spectroscopy. The results showed that AMC was grafted onto the CNC surface by an amidation reaction, and the absorption and emission maxima for FCNC were blue-shifted from 350 nm and 445 nm of AMC to 335 nm and 440 nm, respectively. FCNC retained the crystallinity and nano-topography size of the CNC. The fluorescence intensity, quantum yield, and fluorescence lifetime of FCNC showed the same change law; it first increased and then decreased with an increase in the graft density of AMC from 0.201 to 0.453 AMC molecules per nm(2). The FCNC prepared in this study have good optical properties and can be used in the fields of fluorescent molecular probes and biological imaging.
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spelling pubmed-90368752022-04-26 Fluorescent labeling and characterization of dicarboxylic cellulose nanocrystals prepared by sequential periodate–chlorite oxidation Sun, Xiaozheng Xue, Yanhua Li, Jianye Yang, Yu Bai, Yu Chen, Yujia RSC Adv Chemistry High-performance fluorescent composites are key to the development and improvement of fluorescent molecular probe technology. In this study, cellulose nanocrystals (CNC) with high carboxyl concentrations were prepared via sequential periodate–chlorite oxidation. Then, fluorescent cellulose nanocrystals (FCNC) were prepared by attaching 7-amino-4-methylcoumarin (AMC) onto CNC under 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) catalysis. The morphology and fluorescence properties of FCNC were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, elemental analysis, ultraviolet-visible absorbance, fluorescence spectrophotometry, and fluorescence spectroscopy. The results showed that AMC was grafted onto the CNC surface by an amidation reaction, and the absorption and emission maxima for FCNC were blue-shifted from 350 nm and 445 nm of AMC to 335 nm and 440 nm, respectively. FCNC retained the crystallinity and nano-topography size of the CNC. The fluorescence intensity, quantum yield, and fluorescence lifetime of FCNC showed the same change law; it first increased and then decreased with an increase in the graft density of AMC from 0.201 to 0.453 AMC molecules per nm(2). The FCNC prepared in this study have good optical properties and can be used in the fields of fluorescent molecular probes and biological imaging. The Royal Society of Chemistry 2021-07-14 /pmc/articles/PMC9036875/ /pubmed/35481032 http://dx.doi.org/10.1039/d1ra04812k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Sun, Xiaozheng
Xue, Yanhua
Li, Jianye
Yang, Yu
Bai, Yu
Chen, Yujia
Fluorescent labeling and characterization of dicarboxylic cellulose nanocrystals prepared by sequential periodate–chlorite oxidation
title Fluorescent labeling and characterization of dicarboxylic cellulose nanocrystals prepared by sequential periodate–chlorite oxidation
title_full Fluorescent labeling and characterization of dicarboxylic cellulose nanocrystals prepared by sequential periodate–chlorite oxidation
title_fullStr Fluorescent labeling and characterization of dicarboxylic cellulose nanocrystals prepared by sequential periodate–chlorite oxidation
title_full_unstemmed Fluorescent labeling and characterization of dicarboxylic cellulose nanocrystals prepared by sequential periodate–chlorite oxidation
title_short Fluorescent labeling and characterization of dicarboxylic cellulose nanocrystals prepared by sequential periodate–chlorite oxidation
title_sort fluorescent labeling and characterization of dicarboxylic cellulose nanocrystals prepared by sequential periodate–chlorite oxidation
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036875/
https://www.ncbi.nlm.nih.gov/pubmed/35481032
http://dx.doi.org/10.1039/d1ra04812k
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