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Constructing a highly bioactive tendon-regenerative scaffold by surface modification of tissue-specific stem cell-derived extracellular matrix

Developing highly bioactive scaffold materials to promote stem cell migration, proliferation and tissue-specific differentiation is a crucial requirement in current tissue engineering and regenerative medicine. Our previous work has demonstrated that the decellularized tendon slices (DTSs) are able...

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Autores principales: Ning, Liang-Ju, Cui, Jing, He, Shu-Kun, Hu, Ruo-Nan, Yao, Xuan, Zhang, Yi, Ding, Wei, Zhang, Yan-Jing, Luo, Jing-Cong, Qin, Ting-Wu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036902/
https://www.ncbi.nlm.nih.gov/pubmed/35480863
http://dx.doi.org/10.1093/rb/rbac020
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author Ning, Liang-Ju
Cui, Jing
He, Shu-Kun
Hu, Ruo-Nan
Yao, Xuan
Zhang, Yi
Ding, Wei
Zhang, Yan-Jing
Luo, Jing-Cong
Qin, Ting-Wu
author_facet Ning, Liang-Ju
Cui, Jing
He, Shu-Kun
Hu, Ruo-Nan
Yao, Xuan
Zhang, Yi
Ding, Wei
Zhang, Yan-Jing
Luo, Jing-Cong
Qin, Ting-Wu
author_sort Ning, Liang-Ju
collection PubMed
description Developing highly bioactive scaffold materials to promote stem cell migration, proliferation and tissue-specific differentiation is a crucial requirement in current tissue engineering and regenerative medicine. Our previous work has demonstrated that the decellularized tendon slices (DTSs) are able to promote stem cell proliferation and tenogenic differentiation in vitro and show certain pro-regenerative capacity for rotator cuff tendon regeneration in vivo. In this study, we present a strategy to further improve the bioactivity of the DTSs for constructing a novel highly bioactive tendon-regenerative scaffold by surface modification of tendon-specific stem cell-derived extracellular matrix (tECM), which is expected to greatly enhance the capacity of scaffold material in regulating stem cell behavior, including migration, proliferation and tenogenic differentiation. We prove that the modification of tECM could change the highly aligned surface topographical cues of the DTSs, retain the surface stiffness of the DTSs and significantly increase the content of multiple ECM components in the tECM-DTSs. As a result, the tECM-DTSs dramatically enhance the migration, proliferation as well as tenogenic differentiation of rat bone marrow-derived stem cells compared with the DTSs. Collectively, this strategy would provide a new way for constructing ECM-based biomaterials with enhanced bioactivity for in situ tendon regeneration applications.
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spelling pubmed-90369022022-04-26 Constructing a highly bioactive tendon-regenerative scaffold by surface modification of tissue-specific stem cell-derived extracellular matrix Ning, Liang-Ju Cui, Jing He, Shu-Kun Hu, Ruo-Nan Yao, Xuan Zhang, Yi Ding, Wei Zhang, Yan-Jing Luo, Jing-Cong Qin, Ting-Wu Regen Biomater Research Article Developing highly bioactive scaffold materials to promote stem cell migration, proliferation and tissue-specific differentiation is a crucial requirement in current tissue engineering and regenerative medicine. Our previous work has demonstrated that the decellularized tendon slices (DTSs) are able to promote stem cell proliferation and tenogenic differentiation in vitro and show certain pro-regenerative capacity for rotator cuff tendon regeneration in vivo. In this study, we present a strategy to further improve the bioactivity of the DTSs for constructing a novel highly bioactive tendon-regenerative scaffold by surface modification of tendon-specific stem cell-derived extracellular matrix (tECM), which is expected to greatly enhance the capacity of scaffold material in regulating stem cell behavior, including migration, proliferation and tenogenic differentiation. We prove that the modification of tECM could change the highly aligned surface topographical cues of the DTSs, retain the surface stiffness of the DTSs and significantly increase the content of multiple ECM components in the tECM-DTSs. As a result, the tECM-DTSs dramatically enhance the migration, proliferation as well as tenogenic differentiation of rat bone marrow-derived stem cells compared with the DTSs. Collectively, this strategy would provide a new way for constructing ECM-based biomaterials with enhanced bioactivity for in situ tendon regeneration applications. Oxford University Press 2022-04-20 /pmc/articles/PMC9036902/ /pubmed/35480863 http://dx.doi.org/10.1093/rb/rbac020 Text en © The Author(s) 2022. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ning, Liang-Ju
Cui, Jing
He, Shu-Kun
Hu, Ruo-Nan
Yao, Xuan
Zhang, Yi
Ding, Wei
Zhang, Yan-Jing
Luo, Jing-Cong
Qin, Ting-Wu
Constructing a highly bioactive tendon-regenerative scaffold by surface modification of tissue-specific stem cell-derived extracellular matrix
title Constructing a highly bioactive tendon-regenerative scaffold by surface modification of tissue-specific stem cell-derived extracellular matrix
title_full Constructing a highly bioactive tendon-regenerative scaffold by surface modification of tissue-specific stem cell-derived extracellular matrix
title_fullStr Constructing a highly bioactive tendon-regenerative scaffold by surface modification of tissue-specific stem cell-derived extracellular matrix
title_full_unstemmed Constructing a highly bioactive tendon-regenerative scaffold by surface modification of tissue-specific stem cell-derived extracellular matrix
title_short Constructing a highly bioactive tendon-regenerative scaffold by surface modification of tissue-specific stem cell-derived extracellular matrix
title_sort constructing a highly bioactive tendon-regenerative scaffold by surface modification of tissue-specific stem cell-derived extracellular matrix
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036902/
https://www.ncbi.nlm.nih.gov/pubmed/35480863
http://dx.doi.org/10.1093/rb/rbac020
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