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Development of an ultrafast fluorescent probe for specific recognition of hypochlorous acid and its application in live cells

Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, a novel coumarin-based fluorescent probe, Cou–HOCl, was prepared for the detection of HOCl. The probe exhibited good selectivity over oth...

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Detalles Bibliográficos
Autores principales: Xu, Zhencai, Wang, Xiaofeng, Duan, Tingting, He, Rong, Wang, Fangwu, Zhou, Xuejun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036915/
https://www.ncbi.nlm.nih.gov/pubmed/35481002
http://dx.doi.org/10.1039/d1ra04082k
Descripción
Sumario:Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, a novel coumarin-based fluorescent probe, Cou–HOCl, was prepared for the detection of HOCl. The probe exhibited good selectivity over other analytes, excellent sensitivity with a detection limit of 16 nM, and fast response within 5 s. And further study demonstrated that the probe could be used not only to image exogenous HOCl in various cells, but also to determine the fluctuating levels of HOCl in macrophage cells during inflammation.