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Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans

Bufo bufo gargarizans Cantor are precious medicinal animals in traditional Chinese medicine (TCM). Bufadienolides as the major pharmacological components are generated from the venomous glands of B. bufo gargarizans. Bufadienolides are one type of cardiac aglycone with a six-member lactone ring and...

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Autores principales: Li, Guangli, An, Tianyue, Li, Yu, Yue, Jinyang, Huang, Ruoshi, Huang, Jia, Liang, Jincai, Yao, Wei, Huang, Liufang, Chen, Yidu, Zhang, Rongrong, Ji, Aijia, Duan, Lixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037069/
https://www.ncbi.nlm.nih.gov/pubmed/35480310
http://dx.doi.org/10.3389/fgene.2022.828877
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author Li, Guangli
An, Tianyue
Li, Yu
Yue, Jinyang
Huang, Ruoshi
Huang, Jia
Liang, Jincai
Yao, Wei
Huang, Liufang
Chen, Yidu
Zhang, Rongrong
Ji, Aijia
Duan, Lixin
author_facet Li, Guangli
An, Tianyue
Li, Yu
Yue, Jinyang
Huang, Ruoshi
Huang, Jia
Liang, Jincai
Yao, Wei
Huang, Liufang
Chen, Yidu
Zhang, Rongrong
Ji, Aijia
Duan, Lixin
author_sort Li, Guangli
collection PubMed
description Bufo bufo gargarizans Cantor are precious medicinal animals in traditional Chinese medicine (TCM). Bufadienolides as the major pharmacological components are generated from the venomous glands of B. bufo gargarizans. Bufadienolides are one type of cardiac aglycone with a six-member lactone ring and have properties of antitumor, cardiotonic, tonsillitis, and anti-inflammatory. The biosynthesis of bufadienolides is complex and unclear. This study explored the transcriptome of three different tissues (skin glands, venom glands, and muscles) of B. bufo gargarizans by high-throughput sequencing. According to the gene tissue–specific expression profile, 389 candidate genes were predicted possibly participating in the bufadienolides biosynthesis pathway. Then, BbgCYP11A1 was identified as a cholesterol side chain cleaving the enzyme in engineering yeast producing cholesterol. Furthermore, the catalytic activity of BbgCYP11A1 was studied with various redox partners. Interestingly, a plant NADPH-cytochrome P450 reductase (CPR) from Anemarrhena asphodeloides showed notably higher production than BbgAdx-2A-BbgAdR from B. bufo gargarizans. These results will provide certainly molecular research to reveal the bufadienolides biosynthesis pathway in B. bufo gargarizans.
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spelling pubmed-90370692022-04-26 Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans Li, Guangli An, Tianyue Li, Yu Yue, Jinyang Huang, Ruoshi Huang, Jia Liang, Jincai Yao, Wei Huang, Liufang Chen, Yidu Zhang, Rongrong Ji, Aijia Duan, Lixin Front Genet Genetics Bufo bufo gargarizans Cantor are precious medicinal animals in traditional Chinese medicine (TCM). Bufadienolides as the major pharmacological components are generated from the venomous glands of B. bufo gargarizans. Bufadienolides are one type of cardiac aglycone with a six-member lactone ring and have properties of antitumor, cardiotonic, tonsillitis, and anti-inflammatory. The biosynthesis of bufadienolides is complex and unclear. This study explored the transcriptome of three different tissues (skin glands, venom glands, and muscles) of B. bufo gargarizans by high-throughput sequencing. According to the gene tissue–specific expression profile, 389 candidate genes were predicted possibly participating in the bufadienolides biosynthesis pathway. Then, BbgCYP11A1 was identified as a cholesterol side chain cleaving the enzyme in engineering yeast producing cholesterol. Furthermore, the catalytic activity of BbgCYP11A1 was studied with various redox partners. Interestingly, a plant NADPH-cytochrome P450 reductase (CPR) from Anemarrhena asphodeloides showed notably higher production than BbgAdx-2A-BbgAdR from B. bufo gargarizans. These results will provide certainly molecular research to reveal the bufadienolides biosynthesis pathway in B. bufo gargarizans. Frontiers Media S.A. 2022-04-05 /pmc/articles/PMC9037069/ /pubmed/35480310 http://dx.doi.org/10.3389/fgene.2022.828877 Text en Copyright © 2022 Li, An, Li, Yue, Huang, Huang, Liang, Yao, Huang, Chen, Zhang, Ji and Duan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Li, Guangli
An, Tianyue
Li, Yu
Yue, Jinyang
Huang, Ruoshi
Huang, Jia
Liang, Jincai
Yao, Wei
Huang, Liufang
Chen, Yidu
Zhang, Rongrong
Ji, Aijia
Duan, Lixin
Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
title Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
title_full Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
title_fullStr Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
title_full_unstemmed Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
title_short Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
title_sort transcriptome analysis and identification of the cholesterol side chain cleavage enzyme bbgcyp11a1 from bufo bufo gargarizans
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037069/
https://www.ncbi.nlm.nih.gov/pubmed/35480310
http://dx.doi.org/10.3389/fgene.2022.828877
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