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Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
Bufo bufo gargarizans Cantor are precious medicinal animals in traditional Chinese medicine (TCM). Bufadienolides as the major pharmacological components are generated from the venomous glands of B. bufo gargarizans. Bufadienolides are one type of cardiac aglycone with a six-member lactone ring and...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037069/ https://www.ncbi.nlm.nih.gov/pubmed/35480310 http://dx.doi.org/10.3389/fgene.2022.828877 |
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author | Li, Guangli An, Tianyue Li, Yu Yue, Jinyang Huang, Ruoshi Huang, Jia Liang, Jincai Yao, Wei Huang, Liufang Chen, Yidu Zhang, Rongrong Ji, Aijia Duan, Lixin |
author_facet | Li, Guangli An, Tianyue Li, Yu Yue, Jinyang Huang, Ruoshi Huang, Jia Liang, Jincai Yao, Wei Huang, Liufang Chen, Yidu Zhang, Rongrong Ji, Aijia Duan, Lixin |
author_sort | Li, Guangli |
collection | PubMed |
description | Bufo bufo gargarizans Cantor are precious medicinal animals in traditional Chinese medicine (TCM). Bufadienolides as the major pharmacological components are generated from the venomous glands of B. bufo gargarizans. Bufadienolides are one type of cardiac aglycone with a six-member lactone ring and have properties of antitumor, cardiotonic, tonsillitis, and anti-inflammatory. The biosynthesis of bufadienolides is complex and unclear. This study explored the transcriptome of three different tissues (skin glands, venom glands, and muscles) of B. bufo gargarizans by high-throughput sequencing. According to the gene tissue–specific expression profile, 389 candidate genes were predicted possibly participating in the bufadienolides biosynthesis pathway. Then, BbgCYP11A1 was identified as a cholesterol side chain cleaving the enzyme in engineering yeast producing cholesterol. Furthermore, the catalytic activity of BbgCYP11A1 was studied with various redox partners. Interestingly, a plant NADPH-cytochrome P450 reductase (CPR) from Anemarrhena asphodeloides showed notably higher production than BbgAdx-2A-BbgAdR from B. bufo gargarizans. These results will provide certainly molecular research to reveal the bufadienolides biosynthesis pathway in B. bufo gargarizans. |
format | Online Article Text |
id | pubmed-9037069 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90370692022-04-26 Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans Li, Guangli An, Tianyue Li, Yu Yue, Jinyang Huang, Ruoshi Huang, Jia Liang, Jincai Yao, Wei Huang, Liufang Chen, Yidu Zhang, Rongrong Ji, Aijia Duan, Lixin Front Genet Genetics Bufo bufo gargarizans Cantor are precious medicinal animals in traditional Chinese medicine (TCM). Bufadienolides as the major pharmacological components are generated from the venomous glands of B. bufo gargarizans. Bufadienolides are one type of cardiac aglycone with a six-member lactone ring and have properties of antitumor, cardiotonic, tonsillitis, and anti-inflammatory. The biosynthesis of bufadienolides is complex and unclear. This study explored the transcriptome of three different tissues (skin glands, venom glands, and muscles) of B. bufo gargarizans by high-throughput sequencing. According to the gene tissue–specific expression profile, 389 candidate genes were predicted possibly participating in the bufadienolides biosynthesis pathway. Then, BbgCYP11A1 was identified as a cholesterol side chain cleaving the enzyme in engineering yeast producing cholesterol. Furthermore, the catalytic activity of BbgCYP11A1 was studied with various redox partners. Interestingly, a plant NADPH-cytochrome P450 reductase (CPR) from Anemarrhena asphodeloides showed notably higher production than BbgAdx-2A-BbgAdR from B. bufo gargarizans. These results will provide certainly molecular research to reveal the bufadienolides biosynthesis pathway in B. bufo gargarizans. Frontiers Media S.A. 2022-04-05 /pmc/articles/PMC9037069/ /pubmed/35480310 http://dx.doi.org/10.3389/fgene.2022.828877 Text en Copyright © 2022 Li, An, Li, Yue, Huang, Huang, Liang, Yao, Huang, Chen, Zhang, Ji and Duan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Li, Guangli An, Tianyue Li, Yu Yue, Jinyang Huang, Ruoshi Huang, Jia Liang, Jincai Yao, Wei Huang, Liufang Chen, Yidu Zhang, Rongrong Ji, Aijia Duan, Lixin Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans |
title | Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
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title_full | Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
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title_fullStr | Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
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title_full_unstemmed | Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
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title_short | Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans
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title_sort | transcriptome analysis and identification of the cholesterol side chain cleavage enzyme bbgcyp11a1 from bufo bufo gargarizans |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037069/ https://www.ncbi.nlm.nih.gov/pubmed/35480310 http://dx.doi.org/10.3389/fgene.2022.828877 |
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