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The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation

BACKGROUND: SCH-79797 was recently shown to be a broad-spectrum antibacterial agent with a dual-bactericidal mechanism. However, its anti-biofilm effect remains unknown. PURPOSE: To investigate the effect of SCH-79797 on the biofilm formation of the cariogenic Streptococcus mutans METHODS AND RESULT...

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Detalles Bibliográficos
Autores principales: Zhang, Lingjun, Shen, Yan, Qiu, Lili, Yu, Fangzheng, Hu, Xiangyu, Wang, Min, Sun, Yan, Pan, Yihuai, Zhang, Keke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037171/
https://www.ncbi.nlm.nih.gov/pubmed/35480051
http://dx.doi.org/10.1080/20002297.2022.2061113
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author Zhang, Lingjun
Shen, Yan
Qiu, Lili
Yu, Fangzheng
Hu, Xiangyu
Wang, Min
Sun, Yan
Pan, Yihuai
Zhang, Keke
author_facet Zhang, Lingjun
Shen, Yan
Qiu, Lili
Yu, Fangzheng
Hu, Xiangyu
Wang, Min
Sun, Yan
Pan, Yihuai
Zhang, Keke
author_sort Zhang, Lingjun
collection PubMed
description BACKGROUND: SCH-79797 was recently shown to be a broad-spectrum antibacterial agent with a dual-bactericidal mechanism. However, its anti-biofilm effect remains unknown. PURPOSE: To investigate the effect of SCH-79797 on the biofilm formation of the cariogenic Streptococcus mutans METHODS AND RESULTS: Crystal violet staining, colony forming units count and MTT assays (for cell metabolic activity) revealed that S. mutans biofilm formation was significantly suppressed. In addition, virulence factors, including extracellular polysaccharides (investigated by bacterial/exopolysaccharide staining and the anthrone method) and acid production (investigated by lactic acid and supernatant pH detection) were also inhibited significantly. Moreover, the biofilm inhibitory effect of SCH-79797 was mediated through its repression of bacterial growth and not by a bactericidal effect, which was verified by growth curve and bacterial live/ dead staining, respectively. Quantitative real-time PCR results disclosed that SCH-79797 affected bacterial acid production and tolerance, polysaccharide synthesis and remodeling, biofilm formation and quorum sensing-related gene expression. In addition, SCH-79797 showed good biocompatibility as determined by cytotoxicity assays. CONCLUSION: SCH-79797 had an anti-biofilm effect and showed application prospects in the control of dental caries.
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spelling pubmed-90371712022-04-26 The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation Zhang, Lingjun Shen, Yan Qiu, Lili Yu, Fangzheng Hu, Xiangyu Wang, Min Sun, Yan Pan, Yihuai Zhang, Keke J Oral Microbiol Original Article BACKGROUND: SCH-79797 was recently shown to be a broad-spectrum antibacterial agent with a dual-bactericidal mechanism. However, its anti-biofilm effect remains unknown. PURPOSE: To investigate the effect of SCH-79797 on the biofilm formation of the cariogenic Streptococcus mutans METHODS AND RESULTS: Crystal violet staining, colony forming units count and MTT assays (for cell metabolic activity) revealed that S. mutans biofilm formation was significantly suppressed. In addition, virulence factors, including extracellular polysaccharides (investigated by bacterial/exopolysaccharide staining and the anthrone method) and acid production (investigated by lactic acid and supernatant pH detection) were also inhibited significantly. Moreover, the biofilm inhibitory effect of SCH-79797 was mediated through its repression of bacterial growth and not by a bactericidal effect, which was verified by growth curve and bacterial live/ dead staining, respectively. Quantitative real-time PCR results disclosed that SCH-79797 affected bacterial acid production and tolerance, polysaccharide synthesis and remodeling, biofilm formation and quorum sensing-related gene expression. In addition, SCH-79797 showed good biocompatibility as determined by cytotoxicity assays. CONCLUSION: SCH-79797 had an anti-biofilm effect and showed application prospects in the control of dental caries. Taylor & Francis 2022-04-18 /pmc/articles/PMC9037171/ /pubmed/35480051 http://dx.doi.org/10.1080/20002297.2022.2061113 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zhang, Lingjun
Shen, Yan
Qiu, Lili
Yu, Fangzheng
Hu, Xiangyu
Wang, Min
Sun, Yan
Pan, Yihuai
Zhang, Keke
The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation
title The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation
title_full The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation
title_fullStr The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation
title_full_unstemmed The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation
title_short The suppression effect of SCH-79797 on Streptococcus mutans biofilm formation
title_sort suppression effect of sch-79797 on streptococcus mutans biofilm formation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037171/
https://www.ncbi.nlm.nih.gov/pubmed/35480051
http://dx.doi.org/10.1080/20002297.2022.2061113
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